Results 91 to 100 of about 131,127 (291)

Transient Transfection of Rolling‐Circle Amplified DNA in Biomanufacturing‐Relevant Mammalian Cell Lines: A Comparison of Transfection Conditions for Optimal Protein Expression

Biotechnology and Applied Biochemistry, EarlyView.
ABSTRACT Rolling‐circle amplification (RCA) is a completely synthetic and rapid mechanism for scaling‐up bulk DNA that eliminates requirements for large‐scale bacterial fermentation. Several reports have described the surprising ability of large unprocessed (hyperbranched) rolling circle‐amplified DNA (RCA DNA) to effectively transfect cultured cells ...
Evelina Loghin, Erik Kvam, Lisa Lowery, Nathan Hudson, Jenny Keen, Brian Davis, Roger B. Dodd, Christine Surrette, John Nelson   +8 more
wiley   +1 more source

Quantitation of intracellular NAD(P)H can monitor an imbalance of DNA single strand break repair in base excision repair deficient cells in real time [PDF]

, 2003
DNA single strand breaks (SSBs) are one of the most frequent DNA lesions in genomic DNA generated either by oxidative stress or during the base excision repair pathways.
Asakura, Shoji, Caldecott, Keith W, de Murcia, Gilbert, Hester, Susan D, Nakamura, Jun, Swenberg, James A   +5 more
core   +3 more sources

Lab‐Scale Continuous Biomanufacturing: A Tool for Process Development With Adaptive Strategies for Capture and Virus Inactivation

Biotechnology and Bioengineering, EarlyView.
Schematic representation of the continuous lab‐scale process. ABSTRACT Monoclonal antibodies (mAbs) are nowadays fundamental in treating a wide range of severe diseases, including cancer, infections, or autoimmune disorders. Due to their high specificity, potent activity, and fewer side effects compared to small molecular drugs, the market for mAbs is ...
Thomas Kruse, Fabian Schmitz, Janina Kilian, Jonas Austerjost, Lucas‐Nik Reger, Markus Kampmann   +5 more
wiley   +1 more source

Specific staining of human chromosomes in Chinese hamster x man hybrid cell lines demonstrates interphase chromosome territories [PDF]

, 1985
In spite of Carl Rabl's (1885) and Theodor Boveri's (1909) early hypothesis that chromosomes occupy discrete territories or domains within the interphase nucleus, evidence in favor pf this hypothesis has been limited and indirect so far in higher plants ...
AB Murray, C Rabl, C Zorn, DA Agard, DE Comings, DE Comings, DJ DuPraw, E Therman, F Vogel, GA Rappold, GA Rappold, J Hozier, J Sedat, JJ Leary, JS Lebkowski, JT Finch, JW Littlefield, K Grell, K Sperling, L Avivi, L Hens, L Manuelidis, L Manuelidis, LM Kunkel, M Feldman, M Raith, MD Bennett, PR Langer, PR Langer-Safer, R Hancock, S Wischnitzer, SM Stack, T Cremer, T Cremer, Th Boveri   +34 more
core   +1 more source

Microsatellite markers of water buffalo, Bubalus bubalis - development, characterisation and linkage disequilibrium studies

BMC Genetics, 2009
Background Microsatellite markers are highly polymorphic and widely used in genome mapping and population genetic studies in livestock species. River buffalo, Bubalus bubalis is an economically important livestock species, though only a limited number of
Vaidhegi R, Mishra Manish, Gupta Jalaj, Paranthaman Karthikeyani, Haribaskar Ramachandran, Nishanth Gopala, Kumar Niraj, Nagarajan Muniyandi, Kumar Shantanu, Ranjan Amresh K, Kumar Satish   +10 more
doaj   +1 more source

Quantitative Analysis Reveals Hitchhiking Drives Polysorbate Hydrolase Persistence Via Host Cell Protein–Antibody Interactions

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Polysorbate‐degrading host cell proteins (HCPs) represent a critical challenge in the manufacturing of monoclonal antibody therapeutics due to their potential to persist during downstream processing. While their enzymatic activity has been characterized, the role of direct HCP‐mAb interactions, particularly those involving polysorbate ...
Melanie Maier, Lukas Griesinger, Matthias Franzreb, Simon Kluters   +3 more
wiley   +1 more source

Assessment of the cytotoxicity of chlorhexidine by employing an in vitro mammalian test system

Journal of Dental Sciences, 2014
Background/purpose: Chlorhexidine (CHX), a chlorophenyl biguanide with broad antibacterial action, has been widely used in dentistry. The initial uses of CHX in dentistry were to wash operation site and to disinfect root canals. Recently, the addition of
Yi-Ching Li, Yu-Hsiang Kuan, Tzu-Hsin Lee, Fu-Mei Huang, Yu-Chao Chang   +4 more
doaj   +1 more source

Beyond mammals: the evolution of chewing and other forms of oropharyngeal food processing in vertebrates

Biological Reviews, EarlyView.
ABSTRACT Oropharyngeal food processing exhibits a remarkable diversity among vertebrates, reflecting the evolution of specialised ‘processing centres’ associated with the mandibular, hyoid, and branchial arches. Although studies have detailed various food‐processing strategies and mechanisms across vertebrates, a coherent and comprehensive terminology ...
Daniel Schwarz, Maja Mielke, Stephan Handschuh, Anthony Herrel, Patrick Lemell, Léa Da Cunha, Nicolai Konow   +6 more
wiley   +1 more source

Activation of Ceramidase and Ceramide Kinase by Vanadate via a Tyrosine Kinase–Mediated Pathway

Journal of Pharmacological Sciences, 2010
.: Ceramide, a key molecule in the metabolism of sphingolipids, is converted by ceramidase to sphingosine, and phosphorylated by ceramide kinase to form ceramide-1-phosphate (C1P). In this study, we improved on a method of thin-layer chromatography using
Eiko Tada, Kaori Toyomura, Hiroyuki Nakamura, Hirotsune Sasaki, Takeshi Saito, Masayuki Kaneko, Yasunobu Okuma, Toshihiko Murayama   +7 more
doaj   +1 more source

Scale‐up of a monoclonal antibody CHO fed‐batch production in stirred tank bioreactors: Effect of hydrodynamic conditions and feeding regimen

Biotechnology Progress, EarlyView.
Abstract Key hydrodynamic‐related parameters such as volumetric power input (P/V), impeller configuration, aeration strategy, and maximum gas sparge rate, as well as an appropriate feeding strategy, must be carefully selected to improve production yields in bioreactor.
Lucas Lemire, Sebastian‐Juan Reyes, Yves Durocher, Robert Voyer, Olivier Henry, Phuong Lan Pham   +5 more
wiley   +1 more source