Results 101 to 110 of about 131,127 (291)
Effects of Laser UV-Microirradiation (λ = 2573 A) on Proliferation of Chinese Hamster Cells [PDF]
, 1976 A laser uv-microbeam with a wavelength of 2573 Å having a minimum spot diameter of approx 0.5 μm was used to microirradiate interphase cells of a V-79 subline of Chinese hamster cells.
Cremer, Christoph +3 more
core
Mechanisms involved in the induction of aneuploidy: the significance of chromosome loss
Genetics and Molecular Biology, 2000 The induction of aneuploidy by physical and chemical agents using different test systems was evaluated. The effect of X-rays, caffeine, acetaldehyde, ethanol, diethylstilbestrol, propionaldehyde, and chloral hydrate was studied by chromosome counting in ...
A.I. Seoane, A.M. Güerci, F.N. Dulout
doaj +1 more source
Dynamic pH profiles drive higher cell‐specific and volumetric productivity
Biotechnology Progress, EarlyView.Implementation of dyamic pH profiles for increased CHO cell culture productivity. Abstract Mammalian cell cultures in bioreactors rely heavily on critical process parameter control to ensure optimal growth, productivity, and reproducibility to produce recombinant therapeutic proteins. Culture pH has been shown to be a critical parameter that influences
Stephanie R. Klaubert +6 more
wiley +1 more source
In vitro approach to the study of chronic exposure to low doses of x-rays [PDF]
, 2011 El presente trabajo fue realizado con el fin de estudiar el efecto de dosis bajas y repetidas de radiación sobre dos líneas celulares de la misma especie. Se desarrolló un modelo in vitro para evitar la influencia de los factores de confusión que afectan
Peral Garcia, Pilar +2 more
core +1 more source
Vesivirus 2117 capsids more closely resemble sapovirus and lagovirus particles than other known vesivirus structures [PDF]
, 2017 Vesivirus 2117 is an adventitious agent that in 2009, was identified as a contaminant of CHO cells propagated in bioreactors at a pharmaceutical manufacturing plant belonging to Genzyme.
Bhella, David +8 more
core +1 more source
Enhancing CHO cell recombinant protein production using a perfusion‐directed host evolution approach
Biotechnology Progress, EarlyView.Abstract Clonally derived cell lines generated from Chinese hamster ovary (CHO) cells encounter numerous stressors when cultured in high‐intensity perfusion bioreactors leading to poor process performance. To circumvent this, the ability of CHO cells to adapt to different culture environments was exploited. Here host cells were selected in the presence
Peter Amaya +7 more
wiley +1 more source
Whole-cell Escherichia coli lactate biosensor for monitoring mammalian cell cultures during biopharmaceutical production [PDF]
, 2017 Many high-value added recombinant proteins, such as therapeutic glycoproteins, are produced using mammalian cell cultures. In order to optimise the productivity of these cultures it is important to monitor cellular metabolism, for example the utilisation
Ainsworth, C +5 more
core +1 more source
Animal Models and Experimental Medicine, EarlyView.
Asian Federation of Laboratory Animal Science Associations (AFLAS)
wiley +1 more source
Biotechnology Progress, EarlyView.Abstract A rapid assessment of manufacturability for drug candidates is crucial for advancing a prospective biotherapeutic from a candidate to a bulk drug substance. A lot‐to‐lot approach to manufacturability is adopted where each biologic batch is assessed for manufacturability as a bulk, unfractionated pool.
Solomon Isu +6 more
wiley +1 more source
Metabolic Engineering Communications, 2019 Mathematical modelling can provide precious tools for bioprocess simulation, prediction, control and optimization of mammalian cell-based cultures. In this paper we present a novel method to generate kinetic models of such cultures, rendering complex ...
Erika Hagrot +4 more
doaj +1 more source