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Lymphocyte cultures from Chinese hamster (Cricetulus griseus)
Experientia, 1972Lymphozyten des chinesischen Hamsters (Cricetulus griseus) wurden in verschiedenen Madia mit verschiedenen Zusatzen gezuchtet. Die beste Mitosenausbeute wurde erhalten mit dem Medium R.P.M.I. 1640, bei einem zustz von 10% fotalem Kalbsserum und 6 μl/ml Phytohamagglutinin.
B, de Jong, G J, Anders
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Isolation of cold-sensitive Chinese hamster cells
Molecular and General Genetics MGG, 1975Six cold-sensitive variants have been isolated from Chinese hamster ovary cells by the BUdR-visible light selection technique. The properties of one of these lines have been studied in detail. This line stops dividing immediately after a shift from 39 degrees C to 33 degrees C though its doubling time at 39 degrees C is only slightly longer than that ...
R A, Farber, P, Unrau
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Asynchronous centromere division in the Chinese hamster
Cytogenetic and Genome Research, 1972Asynchronous centromere division, i.e., variation in the distance between sister centromeres in the same cell, has been observed in bone-marrow cells of the Chinese hamster (2n = 22). The cells were harvested with brief hypotonic treatment but without colchicine.
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Osmotic stress variants in Chinese hamster cells
Somatic Cell and Molecular Genetics, 1993Stable variants resistant to hypertonic stress have been obtained from V79 cells by one-step selection in media supplemented with graded concentrations of NaCl. Such variants retain a potential for resistance when isolated and propagated in isotonic media.
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ATM dysfunction in Chinese hamster XRCC8 mutants
Biochemical and Biophysical Research CommunicationsXRCC8 is a member of the X-ray cross-complementing (XRCC) family, whose responsible gene has not been identified. Previous studies suggested ATM and other genes were potential candidates for XRCC8, but this was not confirmed. In this study, we characterized three V79-derived XRCC8 mutant cells: V-C4, V-E5, and V-G8. Western blot analysis showed reduced
Piyawan Chailapakul +2 more
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[1] Chinese hamster ovary cells
1987Publisher Summary This chapter describes Chinese hamster ovary (CHO) cells that use for genetic analysis in tissue culture. These cells have been used for the isolation of mutants affecting intermediary metabolism; DNA, RNA, and protein synthesis; membrane functions; and several more complex forms of cell behavior such as cell growth and endocytosis.
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[Chinese hamster emotionality rating scale].
Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University, 2002To establish the rating method of Chinese Hamster emotionality and verify its reliability of assessment result so as to investigate the role of emotionality in onset/incidence of diabetes in Chinese Hamster of non-insulin-dependent diabetes mellitus, Ader's rating methods of emotionality were revised, the level of emotionality for Chinese Hamster was ...
D, Wu, S, Yao, X, Dai, W, Cai
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Chinese Hamster Ovary Mutation Assays
1984CHO cells are widely used in cell biology, biochemistry, and genetic studies. Their widest application in genetic analysis is probably as target cells for in vitro cytogenetic analysis. The cells are readily available, grow with high cloning efficiency ( > 80%), and have a reproducible generation time of 12–14 h (3).
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Diabetes in the Chinese hamster
1996According to Yerganian,1 the first recorded experimental use of the Chinese hamster, Cricetulus griseus, was for the identification of pneumococcal types prior to treatment of pneumonia at the Peking Medical College Hospital in 1919 because laboratory mice were not available.2 The Chinese or “striped-back” hamster is recognized by the black stripe that
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