Results 71 to 80 of about 5,769,168 (249)
Radiology and OncologyAdministering gadolinium-based contrast agent before electroporation allows the contrast agent to enter the cells and enables MRI assessment of reversibly electroporated regions.
Strucic Marko +5 more
doaj +1 more source
Osmotic Effects Induced by Pore-Forming Agent Nystatin: From Lipid Vesicles to the Cell. [PDF]
PLoS ONE, 2016 The responses of Chinese hamster ovary epithelial cells, caused by the pore-forming agent nystatin, were investigated using brightfield and fluorescence microscopy.
Špela Zemljič Jokhadar +3 more
doaj +1 more source
Biotechnology Progress, EarlyView.Abstract Maintaining consistent quality in the manufacturing of biotherapeutic proteins in mammalian cell culture is challenging, with unplanned deviations causing inconsistencies and potential batch failure. Current methods for monitoring and controlling critical process parameters (CPPs) rely on slow, labor‐intensive offline analyses.
Matthew Banner +12 more
wiley +1 more source
Biotechnology Progress, EarlyView.Abstract High performance countercurrent membrane purification (HPCMP) has emerged as a promising diffusive separation strategy for the purification of recombinant proteins and monoclonal antibodies (mAbs). However, prior studies employed dialysis membranes, which are designed for diffusive separations but have not been validated for biopharmaceutical ...
Aylin Mohammadzadehmarandi +8 more
wiley +1 more source
Optical sorting and photo-transfection of mammalian cells [PDF]
, 2010 Recently, laser light sources of different regimes have emerged as an essential tool in the biophotonics research area. Classic applications include, for example: manipulating single cells and their subcellular organelles, sorting cells in microfluidic ...
Mthunzi, Patience
core +2 more sources
Rapid Determination of Membrane Transport Parameters in Adherent Cells
BioTechniques, 2000 Reported here is a new method that permits rapid (approximately 5 s) determinations of membrane transport phenomena in cells grown in monolayers at the base of 17-mm glass scintillation vials.
Karim A. Sharif, I. David Goldman
doaj +1 more source
Biotechnology Progress, EarlyView.Abstract Development of therapeutic bispecific antibodies (BsAbs) poses significant manufacturability challenges associated with correct chain pairing and an increased diversity of impurities. Here we address this with a product quality assessment during cell line development (CLD), forgoing the need for genetic characterization during cell line ...
Molly Robinson +7 more
wiley +1 more source
High throughput, efficacious gene editing & genome surveillance in Chinese hamster ovary cells.
PLoS ONE, 2019 Chinese hamster ovary (CHO) cells are a common tool utilized in bioproduction and directed genome engineering of CHO cells is of great interest to enhance recombinant cell lines.
S C Huhn, Y Ou, A Kumar, R Liu, Z Du
doaj +1 more source
DNA concentration in harvest affects retrovirus clearance capabilities of protein A chromatography
Biotechnology Progress, EarlyView.Abstract Protein A chromatography is a key unit operation in biopharmaceutical purification, widely used to capture monoclonal antibodies (mAbs) from harvest cell culture fluid (HCCF). In addition to its primary function, protein A chromatography is also frequently tested for its ability to remove viruses. Although protein A specifically binds mAbs and
Lukas Döring +7 more
wiley +1 more source
Bangladesh Journal of Pharmacology, 2013 Pro-apoptotic activity of Tulbghia violacea extracts was evaluated in order to understand the mechanisms of action that might be related to its traditional use as anticancer medicine.
Sylvester Leonard Lyantagaye
doaj +1 more source