Results 61 to 70 of about 5,769,168 (249)

Detrimental effect of zwitterionic buffers on lysosomal homeostasis in cell lines and iPSC-derived neurons [version 1; peer review: 3 approved]

AMRC Open Research, 2020
Good’s buffers are commonly used for cell culture and, although developed to have minimal to no biological impact, they cause alterations in cellular processes such as autophagy and lysosomal enzyme activity. Using Chinese hamster ovary cells and induced
Nicholas D. Allen, Helen Waller-Evans, Emyr Lloyd-Evans, Rafael A. Badell-Grau, Sophie R. Cook, Kimberley M. Jones, Emily D. Kirkham, Jincy Winston, Brendan P. Kelly   +8 more
doaj   +1 more source

Purification of Advanced Therapeutics by Flow‐Through Pseudo‐Affinity Chromatography: Bi‐Specific mABs, Fc Fusion Proteins, and Adeno‐Associated Viruses

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Modern biopharmaceutical manufacturing requires purification platforms capable of processing structurally and functionally diverse products while addressing the challenge of removing persistent and high‐risk host cell proteins (HCPs).
Wenning Chu, Eduardo Barbieri, Arianna Minzoni, Shriarjun Shastry, Jacob P. LeBarre, Qingbo Shu, Taufika Williams, Xuanwen Li, Divya Chandra, Xuankuo Xu, Jerome Bill, David Roush, Joseph Taris, Patrick Gilbert, Andre Kiesewetter, Oliver Rammo, Michael Schulte, Christopher Major, Trina Mouchahoir, Steven Cramer, Cristiana Boi, Ruben G. Carbonell, Abraham Lenhoff, Stefano Menegatti   +23 more
wiley   +1 more source

Autophagy and apoptosis in Chinese hamster ovary cell culture [PDF]

Autophagy, 2008
Upon nutrient deprivation during Chinese hamster ovary (CHO) cell culture for foreign protein production, cells are subjected to two types of programmed cell death (PCD), apoptosis and autophagy. However, only apoptosis has drawn attention in the field of CHO cell culture.
Hwang, SO Hwang, Sun Ok, Lee, GM Lee, Gyun-Min   +1 more
openaire   +2 more sources

CRISPR Technologies in Chinese Hamster Ovary Cell Line Engineering

International Journal of Molecular Sciences, 2023
The Chinese hamster ovary (CHO) cell line is a well-established platform for the production of biopharmaceuticals due to its ability to express complex therapeutic proteins with human-like glycopatterns in high amounts. The advent of CRISPR technology has opened up new avenues for the engineering of CHO cell lines for improved protein production and ...
Katja Glinšek, Krištof Bozovičar, Tomaž Bratkovič   +2 more
openaire   +2 more sources

High Cell Density Cultivation of HEK293 Cells Using a 2L Membrane‐Stirred Bioreactor

Biotechnology and Bioengineering, EarlyView.
ABSTRACT The use of perfusion systems for the continuous cultivation of animal cells at high cell density (HCD) for the production of recombinant proteins, viruses, and viral vectors has many advantages. Besides an enhancement of volumetric productivity and high cell viability, the product quality can be improved through a steady‐state environment ...
Lennart Jacobtorweihe, Yasemin van Heuvel, Kathrina Scheiermann, Patrick Bongartz, Yvonne Genzel, Udo Reichl   +5 more
wiley   +1 more source

Zephgrabetaine: A New Betaine-type Amaryllidaceae Alkaloid from

Natural Product Communications, 2013
Zephgrabetaine (1), a new betaine type Amaryllidaceae alkaloid, along with seven known alkaloids, lycorine, galanthine, lycoramine, hamayne, haemanthamine, tortuosine, and ungeremine were isolated from the bulbs of Zephyranthes grandiflora and their ...
Deepali Katoch, Dharmesh Kumar, Upendra Sharma, Neeraj Kumar, Yogendra S. Padwad, Brij Lal, Bikram Singh   +6 more
doaj   +1 more source

Intracellular trafficking of recycling apolipoprotein E in Chinese hamster ovary cells

Journal of Lipid Research, 2006
We have investigated apolipoprotein E (apoE) recycling in Chinese hamster ovary (CHO) cells, a peripheral cell that does not produce lipoproteins or express apoE.
Nicole A. Braun, Peter J. Mohler, Karl H. Weisgraber, Alyssa H. Hasty, MacRae F. Linton, Patricia G. Yancey, Yan Ru Su, Sergio Fazio, Larry L. Swift   +8 more
doaj   +1 more source

Modulation of Phosducin-Like Protein 3 (PhLP3) Levels Promotes Cytoskeletal Remodelling in a MAPK and RhoA-Dependent Manner [PDF]

, 2011
Background Phosducin-like protein 3 (PhLP3) forms a ternary complex with the ATP-dependent molecular chaperone CCT and its folding client tubulin. In vitro studies suggest PhLP3 plays an inhibitory role in ?-tubulin folding while conversely in vivo ...
C Mark Smales, Nandini V. L. Hayes, Hayes, Nandini V. L., Hayes Nandini V. L., C. Mark Smales, Carden Martin J., Martin J. Carden, Smales, Christopher Mark, Nandini V L Hayes, Carden, Martin J., Lyne Jossé, Smales C. Mark, Jossé Lyne, Jossé, Lyne, Martin J Carden   +14 more
core   +2 more sources

Ferritin as a Nanoparticle Scaffold for Plant‐Produced Next‐Generation Subunit Vaccines

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Protein nanoparticles offer an innovative approach to next‐generation subunit vaccine development by displaying antigenic sequences on the nanoparticle surface. Compared to traditional subunit vaccines, protein nanoparticle vaccines often show improved interaction with the immune system due to their particulate size and repetitive epitope ...
Jordan T. VanderBurgt, Richard Strasser, Christopher P. Garnham, Rima Menassa   +3 more
wiley   +1 more source

Unmasking Glycoforms: Lectin‐Based Profiling and Functional Implications of Targeted Glycosylation Knockouts in Chinese Hamster Ovary Cells

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Monoclonal antibody (mAb) glycosylation is a critical quality attribute that is difficult to rationally engineer and rapidly assess during cell line development. Here, we investigate whether cell‐surface glycosylation can serve as a surrogate readout of mAb product glycosylation following targeted glycogene engineering in CHO cells.
Cristina Abascal Ruiz, Sheryl Li Yan Lim, Jacobus Brink, Sara Carillo, Eoin Casey, Jonathan Bones, Ioscani Jiménez del Val   +6 more
wiley   +1 more source