Results 41 to 50 of about 5,769,168 (249)

One-Step Enrichment of Intact Glycopeptides From Glycoengineered Chinese Hamster Ovary Cells

Frontiers in Chemistry, 2020
Recently, the glycoproteomic analysis of intact glycopeptides has emerged as an effective approach to decipher the glycan modifications of glycoproteins at the site-specific level.
Ganglong Yang, Naseruddin Höti, Shao-Yung Chen, Shao-Yung Chen, Yangying Zhou, Qiong Wang, Michael Betenbaugh, Hui Zhang, Hui Zhang   +8 more
doaj   +1 more source

Salmonella enterica serovar typhi limits the potency of typhoid toxin and ADP‐ribosylating toxin AB to establish a persistent infection

FEBS Open Bio, EarlyView.
The two catalytic subunits of typhoid toxin dissociate from the holotoxin in the ER of an intoxicated cell, but only CdtB exits the ER to generate immunosuppressive effects. PltA is retained in the ER and sequestered from its cytosolic target, thus allowing the anti‐inflammatory effects of CdtB to promote intestinal colonization.
Maria C. Zabala‐Rodriguez, Juliette K. Tinker, Teresa Frisan, Taj Azarian, Ken Teter   +4 more
wiley   +1 more source

Comprehensive Glycoproteomic Analysis of Chinese Hamster Ovary Cells [PDF]

Analytical Chemistry, 2018
The Chinese hamster ovary (CHO) cell line is a major expression system for the production of therapeutic proteins, the majority of which are glycoproteins, such as antibodies and erythropoietin (EPO). The characterization glycosylation profile of therapeutic proteins produced from engineered CHO cells and therapeutic functions, as well as side effects,
Ganglong Yang, Yingwei Hu, Shisheng Sun, Chuanzi Ouyang, Weiming Yang, Qiong Wang, Michael Betenbaugh, Hui Zhang   +7 more
openaire   +2 more sources

Variation in the Thyrotropic Activity of Human Chorionic Gonadotropin in Chinese Hamster Ovary Cells Arises from Differential Expression of the Human Thyrotropin Receptor and Microheterogeneity of the Hormone. [PDF]

, 1995
The role of hCG as a stimulator of the human thyroid has been a subject of controversy, because discrepant results have been obtained in different in vitro assays.
Mann, K., Poertl, S., Liss, I., Amir, S.M., Hoermann, R.   +4 more
core   +1 more source

The Natural Product Corramycin Acts as a DNA Gyrase Poison and Overcomes Fluoroquinolone Resistance in Mycobacterium tuberculosis

Advanced Science, EarlyView.
Corramycin, a myxobacterial natural product antibiotic, exhibits potent bactericidal activity against multidrug‐resistant Mycobacterium tuberculosis. The compound hijacks bacterial transporters such as BacA and OppABCD to enter the cell and inhibits DNA synthesis through a previously unrecognized mode of DNA gyrase poisoning, locking the enzyme in an ...
Franziska Fries, Jennifer Herrmann, Michael Dal Molin, Yaëlle Wormser, Timo Risch, Yunsheng Gao, Gareth Prosser, Francesca Gubellini, Ariel Mechaly, Joshua B. Wallach, Anthony Castro, Arka Banerjee, Sari Rasheed, Alexander Horn, Mathias Müsken, Michael Kurz, Lindsay Sonnenkalb, Viola Dreyer, Stefan Niemann, Sönke Andres, Dirk Schnappinger, Uli Kazmaier, Norbert Reiling, Rolf Müller, Jan Rybniker, Stéphanie Petrella   +25 more
wiley   +1 more source

Effects of Laser UV-Microirradiation (λ = 2573 A) on Proliferation of Chinese Hamster Cells [PDF]

, 1976
A laser uv-microbeam with a wavelength of 2573 Å having a minimum spot diameter of approx 0.5 μm was used to microirradiate interphase cells of a V-79 subline of Chinese hamster cells.
Cremer, Thomas, Schoeller, L., Cremer, Christoph, Zorn, Christian   +3 more
core   +1 more source

A comprehensive proteome and phosphoproteome atlas across nine organs of the Chinese hamster

Animal Models and Experimental Medicine
Background The Chinese hamster (Cricetulus griseus) is not only an important model organism but also the source of Chinese hamster ovary cells, which play an indispensable role in biomedical and biopharmaceutical research.
Luyao Zhang, Tao Ding, Wanjun Peng, Jie Kong, Xiaoyue Tang, Jiyu Xu, Qiaochu Wang, Zhiyi Zhang, Chunmei Shi, Yizhuo Tian, Huilan Yang, Yehong Yang, Yue Wu, Xiaoyan Liu, Jiangfeng Liu, Juntao Yang   +15 more
doaj   +1 more source

Investigating the Glucagon Receptor and Glucagon-Like Peptide 1 Receptor Activity of Oxyntomodulin-Like Analogues in Male Wistar Rats

Current Therapeutic Research, 2015
Aims: To investigate the effect of Glu-3 OXM-like analogues on food intake and bodyweight in male rats. Background: Oxyntomodulin (OXM) is a natural agonist at both the glucagon receptor (GCGr) and the glucagon-like peptide 1 receptor (GLP-1r), and ...
Samantha L. Price, MRes, James S. Minnion, PhD, Stephen R. Bloom, MD   +2 more
doaj   +1 more source

Cell Segmentation Beyond 2D—A Review of the State‐of‐the‐Art

Advanced Intelligent Discovery, EarlyView.
Cell segmentation underpins many biological image analysis tasks, yet most deep learning methods remain limited to 2D despite the inherently 3D nature of cellular processes. This review surveys segmentation approaches beyond 2D, comparing 2.5D and fully 3D methods, analyzing 31 models and 32 volumetric datasets, and introducing a unified reference ...
Fabian Schmeisser, Haseeb Muhammad, Junaid Younas, Gillian Lovell, Bianca Migliori, Andreas Dengel, Sheraz Ahmed   +6 more
wiley   +1 more source

Antibody mix-and-read assays based on fluorescence intensity probes

mAbs, 2021
Antibodies and Fc fusion proteins are a rapidly growing class of pharmaceuticals. Cell culture and purification process development and operation require frequent measurement of product concentrations, commonly by complex enzyme-linked immunosorbent ...
Ujwal Patil, Atul Goyal, Binh Vu, Yanyun Liu, Vijay Maranholkar, Katerina Kourentzi, James M. Briggs, Richard C. Willson   +7 more
doaj   +1 more source