Results 71 to 80 of about 14,636,985 (313)

SPHINX31 acts as a SRPK1 inhibitor targeting the ATR/DNA‐PKcs/CHK1 replicative checkpoint to inhibit cell growth in non‐small cell lung cancer

open access: yesMolecular Oncology, EarlyView.
The kinase SRPK1 directly interacts with the protein TOPBP1 and regulates the pre‐mRNA splicing of WIZ thereby contributing to the activation of the ATR/CHK1 replicative checkpoint in response to replicative stress. This allows cancer cells' genomic stability and survival.
Amani Shreim   +17 more
wiley   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

open access: yesFEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu   +5 more
wiley   +1 more source

The Making of a Chinese Boycott: The Origins of the 1905 Anti-American Movement

open access: yes, 1999
American Journal of Chinese Studies62123 ...
Wong, Sin Kiong
core  

Chinese Association of Greater Toledo newsletter, Fall, 2020

open access: yes, 2020
A newsletter published by the Chinese Association of Greater Toledo (CAGT). CAGT was founded in 1965 to support the Chinese community in and around Toledo, Ohio and uphold Chinese culture, language, and customs.
Chinese Association of Greater Toledo (Toledo, Ohio)
core  

Small RNA pathways in mammalian oocytes

open access: yesFEBS Open Bio, EarlyView.
Three distinct small RNA pathways operate in mammalian oocytes: RNAi interference (RNAi), the microRNA (miRNA) pathway, and the PIWI‐associated RNA (piRNA) pathway. These pathways use small RNAs to guide sequence‐specific repression and contribute to oocyte biology by targeting genes and mobile elements or appear insignificant since different ...
Petr Svoboda, Josef Pasulka
wiley   +1 more source

Effects of IGFBP4 deficiency on human preadipocyte proliferation and differentiation through the IGF1R/AKT pathway

open access: yesFEBS Open Bio, EarlyView.
IGFBP4 knockdown (KD) impairs preadipocyte proliferation and is associated with IGF1R protein downregulation and attenuated AKT phosphorylation. The mechanisms by which IGFBP4 KD influences the IGF1R/AKT signaling pathway involve newly synthesized proteins and lysosomal degradation pathways. Created in BioRender.
Yujia Guo   +6 more
wiley   +1 more source

Dynamics of biomass and carbon sequestration across a chronosequence of Caragana intermedia plantations on alpine sandy land

open access: yesScientific Reports, 2018
Considering the variations in carbon concentrations among different plant components can significantly improve carbon storage estimates. The aim of this study was to estimate the biomass and carbon storage and sequestration in Caragana intermedia Kuang ...
Qingxue Li   +4 more
doaj   +1 more source

Teaching Classical Chinese Poetry through Reception Studies

open access: yesThe ASIANetwork Exchange: A Journal for Asian Studies in the Liberal Arts, 2019
This paper discusses the contribution reception studies can make to the pedagogy of Chinese poetry. It introduces the major theoretical concepts of reception studies, and then demonstrates how those concepts can be applied in and incorporated into ...
Yue Zhang
doaj   +2 more sources

Early Communist China [PDF]

open access: yes, 2020
Contains two detailed case studies. In “The Fu-t’ien Incident, December 1930,” Ronald Suleski describes the pivotal incident in the power struggle between Mao Zedong and the Communist Central Committee.
Suleski, Ronald, Bays, Daniel H.
core   +1 more source

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