Results 11 to 20 of about 1,561 (158)
An Antifungal Chitosanase from Bacillus subtilis SH21 [PDF]
Molecules, 2021 Bacillus subtilis SH21 was observed to produce an antifungal protein that inhibited the growth of F. solani. To purify this protein, ammonium sulfate precipitation, gel filtration chromatography, and ion-exchange chromatography were used.
Yuanxiang Pang +10 more
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Production, statistical evaluation and characterization of chitosanase from Fusarium oxysporum D18
Annals of Microbiology, 2023 Purpose The present research work focuses on the extraction of chitosanase enzyme from soil fungi. Chitosan hydrolysis by chitosanase is one of the most effective methods to produce chitosan oligosaccharides which are new biomaterials that have many ...
Rania M. A. Abedin +2 more
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Structural simulation and protein engineering to convert an endo-chitosanase to an exo-chitosanase [PDF]
Protein Engineering Design and Selection, 2008 To obtain an enzyme for the production of chito-disaccharides (GlcN(2)) by converting endo-chitosanase to exo-chitosanase, we chose an endo-chitosanase from Bacillus circulans MH-K1 (Csn) as the candidate for protein engineering. Using molecular modeling, two peptides with five amino acids (PCLGG) and six amino acids (SRTCKP) were designed and inserted
Yueh-Yun, Yao +8 more
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Frontiers in Microbiology, 2021 Chitosanase plays an important role in enzymatic production of chitosan oligosaccharides (COSs). The present study describes the gene cloning and high-level expression of a high-efficiency chitosanase from Bacillus mojavensis SY1 (CsnBm).
Jianrong Wang +8 more
doaj +1 more source
Reassessment of chitosanase substrate specificities and classification [PDF]
Nature Communications, 2017 AbstractChitosanases can be used to produce partially acetylated chitosan oligosaccharides (paCOS) for different applications, provided they are thoroughly characterized. However, recent studies indicate that the established classification system for chitosanases is too simplistic.
Tobias Weikert +4 more
openaire +3 more sources
Calcium triggers the refolding of Bacillus subtilis chitosanase [PDF]
Biochemical Journal, 2003 We characterized the reversible folding—unfolding transition of Bacillus subtilis exocellular chitosanase from either thermal or urea denaturation of the protein. The transitions were monitored in each case by intrinsic fluorescence changes and resistance to proteolysis.
Anne, Colomer-Pallas +3 more
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Marine Drugs, 2019 Chitosanase has attracted great attention due to its potential applications in medicine, agriculture, and nutraceuticals. In this study, P. mucilaginosus TKU032, a bacterial strain isolated from Taiwanese soil, exhibited the highest chitosanase activity (
Chien Thang Doan +4 more
doaj +1 more source
Cloning and Characterization of a New Chitosanase From a Deep-Sea Bacterium Serratia sp. QD07
Frontiers in Microbiology, 2021 Chitosanase is a significant chitosan-degrading enzyme involved in industrial applications, which forms chitooligosaccharides (COS) as reaction products that are known to have various biological activities.
Qiuling Zheng +5 more
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Conversion of Squid Pens to Chitosanases and Proteases via Paenibacillus sp. TKU042
Marine Drugs, 2018 Chitosanases and proteases have received much attention due to their wide range of applications. Four kinds of chitinous materials, squid pens, shrimp heads, demineralized shrimp shells and demineralized crab shells, were used as the sole carbon and ...
Chien Thang Doan +4 more
doaj +1 more source
Cloning and Characterization of a Cold-adapted Chitosanase from Marine Bacterium Bacillus sp. BY01
Molecules, 2019 Chitosanase plays an important role in the production of chitooligosaccharides (CHOS), which possess various biological activities. Herein, a glycoside hydrolase (GH) family 46 chitosanase-encoding gene, csnB, was cloned from marine bacterium Bacillus sp.
Yue Yang +7 more
doaj +1 more source