Results 11 to 20 of about 2,397 (206)

An Antifungal Chitosanase from Bacillus subtilis SH21 [PDF]

Molecules, 2021
Bacillus subtilis SH21 was observed to produce an antifungal protein that inhibited the growth of F. solani. To purify this protein, ammonium sulfate precipitation, gel filtration chromatography, and ion-exchange chromatography were used.
Yuanxiang Pang, Jianjun Yang, Xinyue Chen, Yu Jia, Tong Li, Junhua Jin, Hui Liu, Linshu Jiang, Yanling Hao, Hongxing Zhang, Yuanhong Xie   +10 more
doaj   +4 more sources

Activity Measurement of Chitosanase by an Amperometric Biosensor [PDF]

Analytical Sciences, 2009
An amperometric biosensor for the detection of glucosamine (GlcN) and chitosan oligosaccharides ((GlcN)n) was introduced for an activity measurement of chitosanase. By using the biosensor, an increase in the anodic current due to the production of GlcN and (GlcN)n by chitosanase was measured in a chitosan solution. The maximum value of the slope of the
TATSUMI, Hirosuke, KATANO, Hajime, IKEDA, Tokuji, KIMOTO, Hisashi   +3 more
openaire   +4 more sources

Cloning and Characterization of a Cold-adapted Chitosanase from Marine Bacterium Bacillus sp. BY01

Molecules, 2019
Chitosanase plays an important role in the production of chitooligosaccharides (CHOS), which possess various biological activities. Herein, a glycoside hydrolase (GH) family 46 chitosanase-encoding gene, csnB, was cloned from marine bacterium Bacillus sp.
Yue Yang, Zhou Zheng, Yifei Xiao, Jiaojiao Zhang, Yu Zhou, Xiao Li, Shangyong Li, Huiqing Yu   +7 more
doaj   +2 more sources

Screening, Identification and Conditions Optimization of High Chitosanase Producing Strains, and Analysis of Its Enzymatic Properties [PDF]

Shipin gongye ke-ji
To screen and identify high chitosanase producing strains and to optimize the fermentation condition and analyze the enzymatic properties of the target strain.
Ying LIANG, Rui LIU, Chaodong SONG, Junjie XIE, Doudou YIN, Xia XU, Dengfeng YANG, Hongyan ZHANG, Naikun SHEN   +8 more
doaj   +2 more sources

Modification of genetic regulation of a heterologous chitosanase gene in Streptomyces lividans TK24 leads to chitosanase production in the absence of chitosan [PDF]

Microbial Cell Factories, 2011
Background Chitosanases are enzymes hydrolysing chitosan, a β-1,4 linked D-glucosamine bio-polymer. Chitosan oligosaccharides have numerous emerging applications and chitosanases can be used for industrial enzymatic hydrolysis of chitosan.
Brzezinski Ryszard, Guay Isabelle, Dubeau Marie-Pierre   +2 more
doaj   +3 more sources

Production of a Thermostable Chitosanase from Shrimp Heads via Paenibacillus mucilaginosus TKU032 Conversion and its Application in the Preparation of Bioactive Chitosan Oligosaccharides

Marine Drugs, 2019
Chitosanase has attracted great attention due to its potential applications in medicine, agriculture, and nutraceuticals. In this study, P. mucilaginosus TKU032, a bacterial strain isolated from Taiwanese soil, exhibited the highest chitosanase activity (
Chien Thang Doan, Thi Ngoc Tran, Van Bon Nguyen, Anh Dzung Nguyen, San-Lang Wang   +4 more
doaj   +2 more sources

Screening for Aspergillus fumigatus strain-2T-2 with high chitosanase production activity and its application in chitosan degradation

Electronic Journal of Biotechnology
Background: High chitosanase-producing microorganisms from natural sources have extensive applications in food and agriculture. This study aimed to optimal conditions for high-activity chitosanase production.
Haoyue Yang, Linsong Wang, Chaojie Xu, Wentong Hao, Ronge Xing, Song Liu, Huahua Yu, Pengcheng Li   +7 more
doaj   +2 more sources

Chitosanase-based method for RNA isolation from cells transfected with chitosan/siRNA nanocomplexes for real-time RT-PCR in gene silencing

International Journal of Nanomedicine, 2010
Mohamad Alameh, Myriam Jean, Diogo DeJesus, Michael D Buschmann, Abderrazzak Merzouki1Institute of Biomedical Engineering, Department of Chemical Engineering, École Polytechnique, Station Centre-ville, Montréal, QC, CanadaAbstract ...
Mohamad Alameh, Myriam Jean, Diogo DeJesus, et al   +3 more
doaj   +1 more source

Multi-Stimuli-Responsive Hydrogen-Bonded Organic Frameworks Nanocarriers Enable Targeted Fungicide Release and Plant Immune Regulation. [PDF]

Adv Sci (Weinh)
A fluorinated hydrogen‐bonded organic framework nanocarrier (PFC‐1‐F) loaded with PYR and coated with HACC enables multi‐stimuli‐responsive (enzyme/ROS/pH) release at infection sites. Exhibiting targeted antifungal activity against Sclerotium rolfsii, modulating plant antioxidant defense, and demonstrating favorable biosafety across plant, soil, and ...
Ma G, Zou Y, Wang Y, Li H, Li J, Liu T, Pan X.   +6 more
europepmc   +2 more sources

Structural simulation and protein engineering to convert an endo-chitosanase to an exo-chitosanase [PDF]

Protein Engineering Design and Selection, 2008
To obtain an enzyme for the production of chito-disaccharides (GlcN(2)) by converting endo-chitosanase to exo-chitosanase, we chose an endo-chitosanase from Bacillus circulans MH-K1 (Csn) as the candidate for protein engineering. Using molecular modeling, two peptides with five amino acids (PCLGG) and six amino acids (SRTCKP) were designed and inserted
Yueh-Yun, Yao, Keshab Lal, Shrestha, Yue-Jin, Wu, Huei-Ju, Tasi, Chun-Chen, Chen, Jinn-Moon, Yang, Akikazu, Ando, Chih-Yu, Cheng, Yaw-Kuen, Li   +8 more
openaire   +2 more sources