Results 51 to 60 of about 1,561 (158)

Engineered Folding and Secretion for Expression Improvement of α‐Amylase in Komagataella Phaffii

Food Bioengineering, Volume 4, Issue 1, Page 3-14, March 2025.
This study enhanced the heterologous expression of α‐amylase in Komagataella phaffii by optimizing the pathways involved in protein secretion and folding, resulting in a yield of 2.5 × 10⁴ U/mL in a 3‐L bioreactor. ABSTRACT α‐Amylase is the second most widely produced enzyme globally, with diverse applications in the fields of food, pharmaceutical ...
Yilun Zhang, Xingbin Wang, Bei Han, Chaoying Yao, Qi Liu, Menghao Cai   +5 more
wiley   +1 more source

Synergistic Effect of Macadamia Nut Oil and Chitosan Coatings on Physicochemical Characteristics of Tomatoes During Storage

Journal of Food Quality, Volume 2025, Issue 1, 2025.
A significant portion of globally produced fruits are lost between the farmer and consumer, necessitating solutions like natural edible coatings for preservation due to their low cost and less toxicity. This study aimed to assess whether the combination of macadamia nut oil and chitosan posed synergistic effects on physicochemical properties of tomato ...
Tlehema Gwandu Umbayda, Anthony Daniel Funga, Alinanuswe Joel Mwakalesi, Ahmed A. Tayel   +3 more
wiley   +1 more source

An Assist for Arthritis Studies: A 3D Cell Culture of Human Fibroblast‐Like Synoviocytes by Encapsulation in a Chitosan‐Based Hydrogel

Advanced Therapeutics, Volume 7, Issue 12, December 2024.
A chitosan‐based hydrogel formulation is developed for 3D culturing of human fibroblast‐like synoviocytes, derived from non‐RA and RA patients. The hydrogel, consisting of 70% chitosan and 30% Matrigel, demonstrates superior stability and cell growth. This cost‐effective system offers a viable alternative to sole Matrigel for synoviocytes encapsulation.
Francesco Bisconti, Beatrice Vilardo, Gaia Corallo, Francesca Scalera, Giuseppe Gigli, Annalisa Chiocchetti, Alessandro Polini, Francesca Gervaso   +7 more
wiley   +1 more source

In Vitro Antagonistic Activity of Plant Growth Promoting Rhizobacteria Against Aggressive Biotypes of the Green Mold

Journal of Basic Microbiology, Volume 64, Issue 12, December 2024.
ABSTRACT During the cultivation of button mushrooms, the green mold epidemic, which causes a decrease in productivity, is a very important problem. The environmental harm of chemicals used in the control of such epidemics and the demand of consumers for organic products without chemicals have brought environmentally friendly biological control to the ...
Baran Mis, Kemal Karaca, Rengin Eltem
wiley   +1 more source

Examining the interplay between Streptococcus agalactiae, the biopolymer chitin and its derivative

MicrobiologyOpen, 2019
Streptococcus agalactiae is a highly pathogenic bacterium of aquatic species and terrestrial animals worldwide, whereas chitin and its derivative chitosan are among the most abundant biopolymers found in nature, including the aquatic milieu.
Mediha Yildirim‐Aksoy, Benjamin H. Beck, Dunhua Zhang   +2 more
doaj   +1 more source

Gene Expression by a Model Fungus in the Ascomycota Provides Insight Into the Decay of Fungal Necromass

Environmental Microbiology, Volume 26, Issue 12, December 2024.
We used RNA sequencing to characterise Trichoderma reesei RUT‐C30 genes and pathways involved in the decomposition of Hyaloscypha bicolor necromass (dead fungal cells and tissues). We showed that T. reesei up‐regulated genes involved in the utilisation of fungal cell wall components such as chitin, glucans, and mannoproteins.
Irshad Ul Haq, Peter Kennedy, Kathryn M. Schreiner, Julia C. Agnich, Jonathan S. Schilling   +4 more
wiley   +1 more source

Potential of natural stimulants and spirulina algae extracts on Cape gooseberry plant: A study on functional properties and enzymatic activity

Food Science &Nutrition, Volume 12, Issue 11, Page 9056-9068, November 2024.
Natural growth stimulants (Kitoplus® and Azotobacter at concentrations of 0% and 0.5%, combined with Spirulina algae extract at concentrations of 0%, 0.25%, and 0.5%) were applied to Cape gooseberry plants. Abstract The need to discover new strategy to enhance agricultural productivity while minimizing negative effects on cultivation system is the ...
Rasoul Heydarnajad Giglou, Mousa Torabi Giglou, Mehrnaz Hatami, Mansour Ghorbanpour   +3 more
wiley   +1 more source

Production, purification, and characterization of an extracellular chitosanase from Streptomyces [PDF]

Journal of Bacteriology, 1975
The synthesis by Streptomyces sp. no. 6 of an extracellular chitosanase was induced by glucosamine. The enzyme was purified to homogeneity by Sephadex G-100, carboxymethyl-cellulose, and diethylaminoethyl-cellulose chromatography. The purified enzyme hydrolyzed chitosan (the beta-1,4-linked polymer of glucosamine) but not chitin nor carboxymethyl ...
J S, Price, R, Storck
openaire   +2 more sources

Production of Chitooligosaccharides and Their Potential Applications in Medicine

Marine Drugs, 2010
Chitooligosaccharides (CHOS) are homo- or heterooligomers of N-acetylglucosamine and D-glucosamine. CHOS can be produced using chitin or chitosan as a starting material, using enzymatic conversions, chemical methods or combinations thereof. Production of
Vincent G. H. Eijsink, Kjell M. Vårum, Morten Sørlie, Anne Line Norberg, Ellinor B. Heggset, Berit B. Aam   +5 more
doaj   +1 more source

Production‐optimized fermentation of antifungal compounds by Bacillus velezensis LZN01 and transcriptome analysis

Microbial Biotechnology, Volume 17, Issue 10, October 2024.
The yields of antimicrobial compounds, such as surfactin, fengycin, shikimic acid, and myriocin, increased or were observed in the optimized fermentation CFS. In conclusion, fermentation conditions improved the antifungal activity of the LZN01 strain and influenced the expression of genes involved in the synthesis of antimicrobial compounds.
Jiale Hu, Zhigang Wang, Weihui Xu
wiley   +1 more source