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Sex determination in Chlamydomonas
Seminars in Cell & Developmental Biology, 2007The sex-determination system of the unicellular green alga, Chlamydomonas reinhardtii, is governed by genes in the mating-type (MT) locus and entails additional genes located in autosomes. Gene expression is initiated by nitrogen starvation, and cells differentiate into plus or minus gametes within 6h.
Ursula, Goodenough +2 more
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The cryopreservation of Chlamydomonas
Cryobiology, 1979A cryophilic strain of the unicellular green alga Chlamydomonas, C. nivalis was found to be more resistant to the stresses both of freezing and thawing and of shrinkage and rehydration than was a mesophilic strain C. reinhardii. C. nivalis was found to have a higher degree of unsaturation of phospholipid fatty acids. Following freezing and thawing of C.
Morris, G.J., Coulson, G., Clarke, A.
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Chlamydomonas (Chlorophyceae) colony PCR
Protoplasma, 2009The ease and effectiveness of colony polymerase chain reaction (PCR) has allowed rapid amplification of DNA fragments and screening of large number of colonies of interest including transformants and mutants with genetic manipulations. Here, we evaluated colony PCR in Chlamydomonas. Individual colonies were treated with 10 mM ethylenediaminetetraacetic
Muqing, Cao +3 more
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Green Alga (Chlamydomonas reinhardtii)
2006This protocol describes the Agrobacterium tumefaciens-mediated nuclear transformation of a microalgae Chlamydomonas reinhardtii, using a gene construct carrying the genes coding for beta-glucuronidase (gus), green fluorescent protein (gfp), and hygromycin phosphotransferase (hpt).
Manchikatla V, Rajam, S Vinod, Kumar
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PHOTOREDUCTION BY CHLAMYDOMONAS
American Journal of Botany, 1954termed "photoreduction" by Gaffron (1940), who has studied this process in some detail particularly in Scenedesmus (Gaffron, 1945). This process has been shown to occur also in a number of other species of green, blue-green, and red algae (Frenkel and Rieger, 1951).
Albert W. Frenkel, Ralph A. Lewin
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Gametic Differentiation of Chlamydomonas
1996Gametogenesis of Chlamydomonas results in the conversion of vegetative cells into gametes. This biological system offers the opportunity to study sexual differentiation at the molecular level in a single cell plant organism. This differentiation is controlled by the consecutive action of the two environmental signals—nitrogen starvation and light.
Beck, C.F., Haring, M.A.
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Axonemal motility in Chlamydomonas
2015Motile cilia and flagella rapidly propagate bending waves and produce water flow over the cell surface. Their function is important for the physiology and development of various organisms including humans. The movement is based on the sliding between outer doublet microtubules driven by axonemal dyneins, and is regulated by various axonemal components ...
Ken-Ichi Wakabayashi, Ritsu Kamiya
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Gamete Behaviour in Chlamydomonas
Nature, 1950Chlamydomonas Moewusii is a heterothallic species showing a difference in behaviour between the gametes of the two mating-types, of a kind not previously recorded.
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Chlamydomonas: Intraflagellar Transport
2017Cilia/flagella are microtubule-based organelles emanating from the surface of most eukaryotic cells and accomplish motile and sensory functions. Malfunction of cilia results in a variety of ciliopathies such as polycystic kidney disease, retinal degeneration, and male infertility.
Gai Liu, Kaiyao Huang
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