Results 191 to 200 of about 43,091 (230)

Cryopreservation ofChlamydomonas reinhardtii(Chlorophyta)

European Journal of Phycology, 1999
Cryopreserved Chlamydomonas reinhardtii cultures remained viable when frozen by cooling slowly to −55°C, then plunging into liquid nitrogen for at least 1 day of storage. High viability (>40%) was retained when cultures contained 2–10% (v/v) methanol as a cryoprotective agent prior to freezing, while dimethyl sulphoxide was ineffective.
ALEXANDRA L. M. CRUTCHFIELD, KENNETH R. DILLER, JERRY J. BRAND   +2 more
openaire   +1 more source

Chlamydomonas reinhardtii Tubulin Gene Structure

Annals of the New York Academy of Sciences, 1986
DNA sequencing studies have provided a picture of the total information available at the gene level for tubulin production in C. reinhardtii. The data indicates that diversity at the gene level is very limited and that all the microtubules in the cell are composed of a very similar set of tubulins.
C D, Silflow, J, Youngblom
openaire   +2 more sources

Biodegradation of phenol by Chlamydomonas reinhardtii

Photosynthesis Research, 2020
The data presented in this particular study demonstrate that the biodegradation of phenol by Chlamydomonas reinhardtii is a dynamic bioenergetic process mainly affected by the production of catechol and the presence of a growth-promoting substrate in the culture medium.
Theocharis T. Nazos, Leonidas Mavroudakis, Spiros A. Pergantis, Demetrios F. Ghanotakis   +3 more
openaire   +2 more sources

The different proteomes of Chlamydomonas reinhardtii

Journal of Proteomics, 2012
Protein identification and proteome mapping mostly rely on the combination of tandem mass spectrometry and sequence database searching. Despite constant improvements achieved in instrumentation, search algorithms, and genome annotations, little effort has been invested in estimating the impact of different genome annotation releases on the final ...
Luis Valledor, Luis Recuenco-Munoz, Volker Egelhofer, ?Stefanie Wienkoop, Wolfram Weckwerth   +4 more
openaire   +4 more sources

The thioredoxin superfamily in Chlamydomonas reinhardtii

Photosynthesis Research, 2004
The thioredoxin (TRX) superfamily includes redox proteins such as thioredoxins, glutaredoxins (GRXs) and protein disulfide isomerases (PDI). These proteins share a common structural motif named the thioredoxin fold. They are involved in disulfide oxido-reduction and/or isomerization.
Stéphane D, Lemaire, Myroslawa, Miginiac-Maslow   +1 more
openaire   +2 more sources

Cellular asymmetry inChlamydomonas reinhardtii

Journal of Cell Science, 1989
ABSTRACTAlthough largely bilaterally symmetric, the two sides of the unicellular alga Chlamydomonas rein-hardtii can be distinguished by the location of the single eyespot. When viewed from the anterior end, the eyespot is always closer to one flagellum than the other, and located at an angle of approximately 45° clockwise of the flagellar plane.
J A, Holmes, S K, Dutcher
openaire   +2 more sources

Chlamydomonas reinhardtii chloroplasts as protein factories

Current Opinion in Biotechnology, 2007
Protein-based therapeutics are the fastest growing sector of drug development, mainly because of the high sensitivity and specificity of these molecules. Their high specificity leads to few side effects and excellent success rates in drug development. However, the inherent complexity of these molecules restricts their synthesis to living cells, making ...
Stephen P, Mayfield, Andrea L, Manuell, Stephen, Chen, Joann, Wu, Miller, Tran, David, Siefker, Machiko, Muto, Julia, Marin-Navarro   +7 more
openaire   +2 more sources

Fluoroacetamide resistance mutations in Chlamydomonas reinhardtii

Archives of Microbiology, 1987
Acetamide, a nitrogen and carbon source for Chlamydomonas reinhardtii, is hydrolyzed by acetamidase to ammonium and acetate. It also induces urea pathway activities. Fluoroacetamide (F-acetamide) is toxic to wildtype through conversion to F-citrate, a respiratory inhibitor. Resistant mutants were selected on plates of F-acetamide plus urea. When tested
R C, Hodson, P M, Gresshoff
openaire   +2 more sources

Efficient H2 production via Chlamydomonas reinhardtii

Trends in Biotechnology, 2011
Molecular hydrogen (H(2)) obtained from biological sources provides an alternative to bulk chemical processes that is moving towards large-scale, economical generation of clean fuel for automotive engines. This opinion article examines recent improvements in H(2) production by wild and mutant strains of Chlamydomonas reinhardtii - the green microalga ...
Maria G, Esquível, Helena M, Amaro, Teresa S, Pinto, Pedro S, Fevereiro, F Xavier, Malcata   +4 more
openaire   +2 more sources

Cyclic nucleotide phosphodiesterase of Chlamydomonas reinhardtii

Biochimica et Biophysica Acta (BBA) - Enzymology, 1974
Abstract Cyclic nucleotide phosphodiesterase (EC 3.1.4.17) activity was characterized in crude and (NH4)2SO4-fractionated extracts of the green alga Chlamydomonas reinhardtii. With cyclic AMP as substrate, the formation of 5′-AMP was optimal at pH 8.5 and required the presence of a sulfhydryl reagent and a divalent cation, Mg2+ and Mn2+ being the ...
U, Fischer, N, Amrhein
openaire   +2 more sources