FISH Mapping and Identification of Zhikong Scallop (Chlamys farreri) Chromosomes
Marine Biotechnology, 2007Chromosome identification is the first step in genomic research of a species, but it remains a challenge in scallops. In the present study, fluorescence in situ hybridization (FISH) mapping of 19 fosmid clones was attempted and used for chromosome identification in Zhikong scallop (Chlamys farreri Jones et Preston, 1904).
Lingling, Zhang +4 more
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The versatile functions of LRR-only proteins in mollusk Chlamys farreri
Developmental & Comparative Immunology, 2017Leucine-rich repeat (LRR)-only proteins are involved in the innate immune responses as they mediate protein-ligand interactions. In the present study, three novel LRR-only proteins, CfLRRop-4, CfLRRop-5 and CfLRRop-6, were identified and characterized from Zhikong scallop Chlamys farreri. They all contained LRR motifs with consensus signature sequences
Mengqiang Wang +6 more
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Cloning and mineralization-related functions of the calponin gene in Chlamys farreri
Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 2016Calponin is a widely distributed protein which is associated with the bio-mineralization process in vertebrates. Recently, a new type of calponin has been found in shellfish; the present study aimed to determine if this gene in shellfish functions in bio-mineralization, one of the most important processes in a mollusk's growth. We chose Chlamys farreri,
Jun, Wang +7 more
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Characterisation of monoclonal antibodies to haemocyte types of scallop (Chlamys farreri)
Fish & Shellfish Immunology, 2005Four monoclonal antibodies (MAbs) (1E7, 1F12, 2H5, 2C6) against haemocytes of scallop (Chlamys farreri) were produced by immunising Balb/C mice. Analysed by the indirect immunofluorescence assay test (IIFAT), immunocytochemical assay, flow cytometry (FCM) and Western-blotting, they showed specificity for more than one haemocyte type (hyalinocyte and ...
Xing, Jing, Zhan, Wenbin
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Evidence for the presence of sex steroid hormones in Zhikong scallop, Chlamys farreri
The Journal of Steroid Biochemistry and Molecular Biology, 2014To obtain evidence of the presence of sex steroid hormones in mollusks, hormone variation in the gonads of the Zhikong scallop Chlamys farreri was analyzed using UPLC-MS/MS. These were found, as expected, with concentrations of estrone (E1), 17beta-estradiol (E2), and testosterone (T) in the testes ranging from not detected (ND) to 0.07 ± 0.10, ND to 3.
Bing-Hui, Zheng +4 more
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Cadmium (Cd) is a hazardous environmental contaminant, which has a serious effect on the ecosystem, food safety and human health. Scallop could accumulate high concentration of Cd from the environment and has been regarded as a Cd hyper-accumulator.
Yanfang Zhao +6 more
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The phenoloxidase activity and antibacterial function of a tyrosinase from scallop Chlamys farreri
Fish & Shellfish Immunology, 2012Tyrosinase (TYR), also known as monophenol monooxygenase, is a ubiquitous binuclear copper-containing enzyme which catalyzes the hydroxylation of phenols to catechols and the oxidation of catechols to quinones. In the present study, the cDNA of a tyrosinase (CfTYR) was identified from scallop Chlamys farreri, which encoded a polypeptide of 486 amino ...
Zhi, Zhou +8 more
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The fragmentation mechanism and immune-protective effect of CfTEP in the scallop Chlamys farreri
Developmental & Comparative Immunology, 2017Thioester-containing proteins (TEPs), characterized by a unique intrachain β-cysteinyl-γ-glutamyl thioester bond, form an ancient and diverse family of secreted proteins that play central roles in the innate immune response. But the existence form and immune protection mechanism of TEP in invertebrates still remain unclear, especially in the mollusks ...
Zhuang, Xue +7 more
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A novel serine protease with clip domain from scallop Chlamys farreri
Molecular Biology Reports, 2007The serine proteases with clip domain are involved in various innate immune functions in invertebrate such as antimicrobial activity, cell adhesion, pattern recognition and regulation of the prophenoloxidase system. A serine protease with clip-domain cDNA (Cf SP) was obtained by Expressed sequence taggings (ESTs) method and rapid amplification of cDNA ...
Ling, Zhu +5 more
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An opioid growth factor receptor (OGFR) for [Met5]-enkephalin in Chlamys farreri
Fish & Shellfish Immunology, 2013Opioid growth factor receptor (OGFR) is a receptor for [Met(5)]-enkephalin and plays important roles in the regulation of cell growth and embryonic development. In the present study, a cDNA of 2381 bp for the scallop Chlamys farreri OGFR (designated as CfOGFR) was identified by rapid amplification of cDNA ends (RACE) approach and expression sequence ...
Ying, Guo +7 more
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