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Chloroplast Transformation

2005
In this chapter we briefly review the developmental history and current research status of chloroplast transformation and introduce the merits of chloroplast transformation as compared with the nuclear genome transformation. Furthermore, according to the chloroplast transformation achieved in oilseed rape (Brassica napus), we introduce the preparation ...
Xiao-Mei, Lu, Wei-Bo, Yin, Zan-Min, Hu
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Chloroplast immunophilins

Protoplasma, 2015
Immunophilins occur in almost all living organisms. They are ubiquitously expressed proteins including cyclophilins, FK506/rapamycin-binding proteins, and parvulins. Their functional significance in vascular plants is mostly related to plant developmental processes, signalling, and regulation of photosynthesis.
Ana, Tomašić Paić, Hrvoje, Fulgosi
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Thioredoxins in chloroplasts

Current Genetics, 2007
Thioredoxins (TRXs) are small disulfide oxidoreductases of ca. 12 kDa found in all free living organisms. In plants, two chloroplastic TRXs, named TRX f and TRX m, were originally identified as light dependent regulators of several carbon metabolism enzymes including Calvin cycle enzymes.
Lemaire SD   +4 more
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THE ELECTROPHOTOLUMINESCENCE OF CHLOROPLASTS

Photochemistry and Photobiology, 1976
Abstract— Delayed light emission emanating from preilluminated chloroplasts can be perturbed with pulsed DC electric fields (200–4000 V cm‐1), The perturbation produces a strong stimulation of chlorophyll luminescence. During the field perturbation the stimulated emission rises to a maximum, typically within 100μs. and then decays.
J L, Ellenson, K, Sauer
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The ultrastructure of chloroplasts

Experimental Cell Research, 1955
1. 1. On ultrathin sections of chloroplasts from Aspidistra elatior the electron microscopic study has revealed that the grana consist of layered columns. 2. 2. The grana are organized in double membrane discs the two membranes of which are about 65 A thick. These membranes bound a narrow, closed space the height of which also is about 65 A. The
E, STEINMANN, F S, SJOSTRAND
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Cytochromes in Chloroplasts

Nature, 1958
IT has been concluded from experiments with barley leaves that chloroplasts contain cytochromes ƒ and b 6 and no cytochrome c 1. We have been able to extend the observations to two other widely differing species and to record certain characteristics of the cytochromes present.
W O, JAMES, R M, LEECH
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isolation of Wheat Chloroplasts and Chloroplast DNA

2003
Chloroplasts in common with other plastids and mitochondria have their own nucleic material, in the form of multiple copies of a circular DNA molecule, normally of 120-160 kbp.
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The chloroplast genome

Plant Molecular Biology, 1992
The chloroplast genome consists of homogeneous circular DNA molecules. To date, the entire nucleotide sequences (120-190 kbp) of chloroplast genomes have been determined from eight plant species. The chloroplast genomes of land plants and green algae contain about 110 different genes, which can be classified into two main groups: genes involved in gene
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Iodination of chloroplasts. I. Properties of iodinated chloroplasts

Biochimica et Biophysica Acta (BBA) - Bioenergetics, 1976
Spinach chloroplasts exposed to iodide can be washed free of the bulk of the iodide. In the presence of lactoperoxidase and H2O2, iodide can be introduced into chloroplasts in high amounts and in non diffusible forms. The resultant particles, which have been named iodochloroplasts, extrude their iodide upon stimulation by light. The form and the amount
E S, Canellakis, G, Akoyunoglou
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Isolation of chloroplasts and chloroplast DNA

1994
That chloroplasts are green and therefore recognizable by eye should have been an advantage in developing methods for their isolation, but historically it has been a disadvantage. It was relatively easy to grind up cells, subject the brei to differential centrifugation, and obtain a green pellet.
C. A. Price   +3 more
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