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Chloroplast DNA systematics: a review of methods and data analysis
, 1994The field of plant molecular systematics is expanding rapidly, and with it new and refined methods are coming into use. This paper reviews recent advances in experimental methods and data analysis, as applied to the chloroplast genome.
R. Olmstead, J. Palmer
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Chloroplast biosystematics: Chloroplast DNA as a molecular probe
Biosystems, 1985The classification of plants has traditionally been dependent upon the comparative analysis of morphological and biochemical data. In this paper the use of molecular probe analysis of chloroplast DNA (ctDNA) is used to expand the data base used in taxonomic studies.
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Nicotiana chloroplast genome III. Chloroplast DNA evolution
Theoretical and Applied Genetics, 1982Nicotiana chloroplast genomes exhibit a high degree of diversity and a general similarity as revealed by restriction enzyme analysis. This property can be measured accurately by restriction enzymes which generate over 20 fragments. However, the restriction enzymes which generate a small number (about 10) of fragments are extremely useful not only in ...
G. F. Shen, Y. S. Zhu, S. D. Kung
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Trends in Biochemical Sciences, 1978
Twenty years after the discovery that chloroplasts contain DNA we still do not know the significance of approximately 90% of this DNA.
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Twenty years after the discovery that chloroplasts contain DNA we still do not know the significance of approximately 90% of this DNA.
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Hybridization of bean chloroplast transfer RNAs to chloroplast DNA
Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1976Bean (Phaseolus vulgaris) chloroplast tRNAsLeu and tRNAsPhe hybridize to chloroplast DNA, whereas the corresponding cytoplasmic tRNA species do not, suggesting that chloroplast transfer RNAs are coded for by chloroplast DNA. The hybridization of the three chloroplast tRNAsLeu or of the two tRNAsPhe isoacceptors is not additive, and the isoacceptors ...
A Steinmetz, J H Well
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1977
We had previously reported the isolation of circular chloro-plast (et) DNA molecules from pea leaves. Circular pea ctDNA was found to have a molecular weight of 90×106 with no evidence of inter- or intramolecular heterogeneity. Recently we have extensively studied the size and structure of ctDNAs from pea, bean, spinach, lettuce, corn, and oats.2 As ...
Nathan M. Chu +3 more
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We had previously reported the isolation of circular chloro-plast (et) DNA molecules from pea leaves. Circular pea ctDNA was found to have a molecular weight of 90×106 with no evidence of inter- or intramolecular heterogeneity. Recently we have extensively studied the size and structure of ctDNAs from pea, bean, spinach, lettuce, corn, and oats.2 As ...
Nathan M. Chu +3 more
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The DNA of Chloroplasts, Mitochondria, and Centrioles
1967Publisher Summary The chapter discusses the evidence for the following conclusions: (1) the cytoplasmic organelles that contain small amounts of deoxyribonucleic acid (DNA); (2) each type of organelle contains its own characteristic DNA; (3) the DNA of the organelles is double-stranded and replicates in a semiconservative fashion; (4) the DNA codes ...
S Granick, Aharon Gibor
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isolation of Wheat Chloroplasts and Chloroplast DNA
2003Chloroplasts in common with other plastids and mitochondria have their own nucleic material, in the form of multiple copies of a circular DNA molecule, normally of 120-160 kbp.
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Roots: The discovery of chloroplast DNA [PDF]
BioEssays, 1986 AbstractIn the space of three years–from 1962 to 1964 – the proposition that chloroplasts contain their own DNA made the transition from being a controversial hypothesis to an accepted dogma. The crucial evidence came from biochemical analyses of the organelles themselves and from cytological studies.
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Isolation of Maize Chloroplasts and Chloroplast DNA
1994This procedure is for the isolation of chloroplasts and highly purified chloroplast DNA from 1 kg of maize seedling leaves. Because starch reduces the yield of intact chloroplasts, light-grown seedlings are placed into darkness for 1–2 days prior to DNA isolation. All manipulations should be performed at 4°C, unless otherwise noted.
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