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Chloroplast DNA from Tobacco Leaves

Science, 1966
DNA from tobacco leaf chloroplasts was isolated as a single component with a buoyant density in CsCl of 1.702 compared to 1.697 for nuclear DNA. 5-Methylcytosine is present in nuclear DNA but absent in chloroplast DNA. Chloroplast DNA, with a guanine-cytosine content of 43 percent, has a melting temperature of 86°C and renatures completely
K K, Tewari, S G, Wildman
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Nicotiana chloroplast genome III. Chloroplast DNA evolution

Theoretical and Applied Genetics, 1982
Nicotiana chloroplast genomes exhibit a high degree of diversity and a general similarity as revealed by restriction enzyme analysis. This property can be measured accurately by restriction enzymes which generate over 20 fragments. However, the restriction enzymes which generate a small number (about 10) of fragments are extremely useful not only in ...
S D, Kung, Y S, Zhu, G F, Shen
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Chloroplast DNA from broad bean

Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1969
Abstract Chloroplasts purified by centrifugation through the glycerol density gradients of James and Das 19 followed by the flotation technique of Jagendorf 20 were free from nuclear contamination. DNA was isolated from pure chloroplasts and nuclei of broad bean.
S D, Kung, J P, Williams
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Chloroplast DNA from three archegoniates

Planta, 1980
1. DNA from female and male Sphaerocarpos donnellii (liverwort) plants exhibits at least two species with buoyant densities of 1.703 (main band) and 1.691 (satellite) g cm(-3) in CsCl equilibrium gradients. At least part, if not all, of the satellite DNA is localized in plastids.
R G, Herrmann, H K, Palta, K V, Kowallik
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Chloroplast DNA inheritance in Populus

Theoretical and Applied Genetics, 1992
The inheritance of chloroplast (cp) DNA was examined in F1 hybrid progenies of two Populus deltoides intraspecific controlled crosses and three P. deltoides × P. nigra and two P. deltoides × P. maximowiczii interspecific controlled crosses by restriction fragment analysis.
O P, Rajora, B P, Dancik
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DNA Biosynthesis in Chloroplasts

Journal of Experimental Botany, 1988
Etude de l'incorporation de TTP tritie dans l'ADN par des extraits chloroplastiques obtenus a differents stades du developpement ...
BRIAN J. BAUMGARTNER   +3 more
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Hybridization of bean chloroplast transfer RNAs to chloroplast DNA

Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1976
Bean (Phaseolus vulgaris) chloroplast tRNAsLeu and tRNAsPhe hybridize to chloroplast DNA, whereas the corresponding cytoplasmic tRNA species do not, suggesting that chloroplast transfer RNAs are coded for by chloroplast DNA. The hybridization of the three chloroplast tRNAsLeu or of the two tRNAsPhe isoacceptors is not additive, and the isoacceptors ...
A, Steinmetz, J H, Well
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Isolation of chloroplasts and chloroplast DNA

1994
That chloroplasts are green and therefore recognizable by eye should have been an advantage in developing methods for their isolation, but historically it has been a disadvantage. It was relatively easy to grind up cells, subject the brei to differential centrifugation, and obtain a green pellet.
C. A. Price   +3 more
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Chloroplast biosystematics: Chloroplast DNA as a molecular probe

Biosystems, 1985
The classification of plants has traditionally been dependent upon the comparative analysis of morphological and biochemical data. In this paper the use of molecular probe analysis of chloroplast DNA (ctDNA) is used to expand the data base used in taxonomic studies.
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