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DNA Synthesis in Chloroplasts

Journal of Experimental Botany, 1989
The influence of two DNA gyrase inhibitors, nalidixic acid and novobiocin on DNA synthesis in isolated pea chloroplasts was examined by autoradiography and ...
W. RONALD MILLS   +3 more
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isolation of Wheat Chloroplasts and Chloroplast DNA

2003
Chloroplasts in common with other plastids and mitochondria have their own nucleic material, in the form of multiple copies of a circular DNA molecule, normally of 120-160 kbp.
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Replication of Chloroplast DNA of Tobacco

Science, 1966
An experimental method has been designed for determining the relative rates of replication of the chloroplast and nuclear DNA's of Nicotiana tabacum . By this method chloroplast DNA in week-old seedlings is being replicated several times faster than nuclear DNA.
B R, Green, M P, Gordon
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The chloroplast DNAs of flax genotrophs

Plant Molecular Biology, 1982
The chloroplast DNA of L. usitatissimum var. "Stormont Cirrus" has been mapped with respect to the recognition sites for the enzymes SalP1, Sst1 and SalG1. It is a circular molecule of about 160 kilobasepairs, with an inverted repeat containing the rDNA.
D, Coates, C A, Cullis
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Insertion polymorphism in pea chloroplast DNA

Theoretical and Applied Genetics, 1985
The chloroplast DNA of higher plants is suitable for restriction endonuclease analysis due to its size and homogeneity. We have analysed 48 different cultivars of pea (Pisum sativum) with EcoRI and HindIII. Of these, only 24 show the standard genotype, the remaining 24 comprise four different classes of short insertions, three of which are found at the
T H, Teeri, A, Saura, J, Lokki
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Chloroplast DNA Topoisomerase I from Cauliflower

The Journal of Biochemistry, 1991
An ATP-independent DNA topoisomerase has been isolated from chloroplasts of cauliflower leaves (Brassica oleracea var. botrytis) through DEAE-cellulose, AF-blue Toyopearl, and hydroxyapatite column chromatography. The sedimentation coefficient and Stokes radius of this enzyme are 3.6S and 3.6 nm, respectively, and the molecular weight of native enzyme ...
H, Fukata   +3 more
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Light-stimulated synthesis of chloroplast DNA

Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1976
Light-stimulated chloroplast DNA synthesis was studied during chloroplast development in the absence of cell division and nuclear DNA synthesis. Incorporation of 32Pi was stimulated 10-15 fold, however, the ratio of chloroplast DNA to nuclear DNA remained constant.
L, Stolarsky   +3 more
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Chloroplast DNA and cytoplasmic male-sterility

Theoretical and Applied Genetics, 1983
This paper deals with the relationship of chloroplast DNA (ctDNA) and cytoplasmic malesterility (CMS) of maize, wheat and rape. Intramolecular heterogeneity and fragment patterns of DNA digested with restriction endonucleases were compared and analyzed. For this purpose methods of agarose gel electrophoresis and two dimensional gel electrophoresis with
L, Jigeng, L, Yi-Nong
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Structure of Chloroplast DNA

1977
We had previously reported the isolation of circular chloro-plast (et) DNA molecules from pea leaves. Circular pea ctDNA was found to have a molecular weight of 90×106 with no evidence of inter- or intramolecular heterogeneity. Recently we have extensively studied the size and structure of ctDNAs from pea, bean, spinach, lettuce, corn, and oats.2 As ...
K. K. Tewari   +3 more
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The chloroplast DNA mystery

Trends in Biochemical Sciences, 1978
Twenty years after the discovery that chloroplasts contain DNA we still do not know the significance of approximately 90% of this DNA.
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