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Roots: The discovery of chloroplast DNA [PDF]

BioEssays, 1986
AbstractIn the space of three years–from 1962 to 1964 – the proposition that chloroplasts contain their own DNA made the transition from being a controversial hypothesis to an accepted dogma. The crucial evidence came from biochemical analyses of the organelles themselves and from cytological studies.
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Isolation of Maize Chloroplasts and Chloroplast DNA

1994
This procedure is for the isolation of chloroplasts and highly purified chloroplast DNA from 1 kg of maize seedling leaves. Because starch reduces the yield of intact chloroplasts, light-grown seedlings are placed into darkness for 1–2 days prior to DNA isolation. All manipulations should be performed at 4°C, unless otherwise noted.
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The characteristics of spinach chloroplast DNA polymerase

Archives of Biochemistry and Biophysics, 1969
Purified chloroplast preparations from spinach incorporate [3H]-dTTP1 into an acid-insoluble product. The incorporation reaction is highly dependent on all four deoxynucleoside triphosphates, Mg2+ and a sulphydryl reagent, and is further stimulated by KCl.
Donald Spencer, Paul R. Whitfeld
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A Chloroplast DNA Study of the Agavaceae

Systematic Botany, 1995
A chloroplast DNA restriction site analysis of 40 taxa in the Agavaceae s.l. was undertaken to test the accuracy of several current systems of classification. In Cronquist's system, the Agavaceae contain 18 genera of fibrous-leaved, more or less woody plants such as Yucca, Hes- peraloe, Agave, Manfreda, Polianthes, Dracaena, Sansevieria, Nolina ...
David J. Bogler, Beryl B. Simpson
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Miniprep for Chloroplast DNA Isolation

Microchemical Journal, 1993
Abstract This paper describes a procedure for the isolation and purification of chloroplastic DNA (cpDNA) designed for undergraduate research projects. It is a modification of the procedures from Doyle and Doyle ( Phytochem. Bull. , 1987, 19 , 11-15), Marmur ( Mol. Biol.
E.P. Oharamays, J.C. Capwell
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Chloroplast DNA from three archegoniates

Planta, 1980
1. DNA from female and male Sphaerocarpos donnellii (liverwort) plants exhibits at least two species with buoyant densities of 1.703 (main band) and 1.691 (satellite) g cm(-3) in CsCl equilibrium gradients. At least part, if not all, of the satellite DNA is localized in plastids.
Reinhold G. Herrmann, Harinder K. Palta, Klaus V. Kowallik   +2 more
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Intramolecular heterogeneity of mitochondrial and chloroplastic DNA

Biochemical and Biophysical Research Communications, 1972
Summary Mitochondrial and chloroplastic DNA from unicellular and higher organisms are splitted into several bands by Cs 2 SO 4 density gradient ultracentrifugations in the presence of Ag + ions. The banding pattern includes a main band DNA, a heavy satellite DNA and, in the most cases, a light satellite DNA.
M. Bayen, Jacques Dalmon, A. Rode, Francis Quetier, F. Vedel   +4 more
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Chloroplast DNA from Tobacco Leaves

Science, 1966
DNA from tobacco leaf chloroplasts was isolated as a single component with a buoyant density in CsCl of 1.702 compared to 1.697 for nuclear DNA. 5-Methylcytosine is present in nuclear DNA but absent in chloroplast DNA. Chloroplast DNA, with a guanine-cytosine content of 43 percent, has a melting temperature of 86°C and renatures completely
K. K. Tewari, Sam G. Wildman
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Studies on Acetabularia chloroplast DNA

1974
The physical properties and renaturation kinetics of DNA extracted from isolated chloroplasts of Acetabulavia meditewanea has been studied. 3 It has a buoyant density of 1.702 g/cm³ , which corresponds to a base composition of 42.8% G+C. When melted in SSC, Acetabulavia chloroplast DNA has a Tm of 86.7°, corresponding to a base composition of 43% G+C ...
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Isolation and Structural Analysis of Chloroplast DNA

1986
Publisher Summary This chapter describes a filter hybridization approach for mapping chloroplast DNA (cpDNA) restriction sites along with several strategies for generating restriction fragments for use as hybridization probes. Approaches for visualizing and mapping cpDNAs that are difficult or impossible to isolate in very pure form are also ...
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