Results 341 to 350 of about 2,161,916 (382)
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1977
We had previously reported the isolation of circular chloro-plast (et) DNA molecules from pea leaves. Circular pea ctDNA was found to have a molecular weight of 90×106 with no evidence of inter- or intramolecular heterogeneity. Recently we have extensively studied the size and structure of ctDNAs from pea, bean, spinach, lettuce, corn, and oats.2 As ...
Nathan M. Chu +3 more
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We had previously reported the isolation of circular chloro-plast (et) DNA molecules from pea leaves. Circular pea ctDNA was found to have a molecular weight of 90×106 with no evidence of inter- or intramolecular heterogeneity. Recently we have extensively studied the size and structure of ctDNAs from pea, bean, spinach, lettuce, corn, and oats.2 As ...
Nathan M. Chu +3 more
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Hybridization of bean chloroplast transfer RNAs to chloroplast DNA
Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1976Bean (Phaseolus vulgaris) chloroplast tRNAsLeu and tRNAsPhe hybridize to chloroplast DNA, whereas the corresponding cytoplasmic tRNA species do not, suggesting that chloroplast transfer RNAs are coded for by chloroplast DNA. The hybridization of the three chloroplast tRNAsLeu or of the two tRNAsPhe isoacceptors is not additive, and the isoacceptors ...
A Steinmetz, J H Well
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Trends in Biochemical Sciences, 1978
Twenty years after the discovery that chloroplasts contain DNA we still do not know the significance of approximately 90% of this DNA.
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Twenty years after the discovery that chloroplasts contain DNA we still do not know the significance of approximately 90% of this DNA.
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The DNA of Chloroplasts, Mitochondria, and Centrioles
1967Publisher Summary The chapter discusses the evidence for the following conclusions: (1) the cytoplasmic organelles that contain small amounts of deoxyribonucleic acid (DNA); (2) each type of organelle contains its own characteristic DNA; (3) the DNA of the organelles is double-stranded and replicates in a semiconservative fashion; (4) the DNA codes ...
S Granick, Aharon Gibor
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Roots: The discovery of chloroplast DNA [PDF]
BioEssays, 1986 AbstractIn the space of three years–from 1962 to 1964 – the proposition that chloroplasts contain their own DNA made the transition from being a controversial hypothesis to an accepted dogma. The crucial evidence came from biochemical analyses of the organelles themselves and from cytological studies.
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Isolation of Maize Chloroplasts and Chloroplast DNA
1994This procedure is for the isolation of chloroplasts and highly purified chloroplast DNA from 1 kg of maize seedling leaves. Because starch reduces the yield of intact chloroplasts, light-grown seedlings are placed into darkness for 1–2 days prior to DNA isolation. All manipulations should be performed at 4°C, unless otherwise noted.
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The characteristics of spinach chloroplast DNA polymerase
Archives of Biochemistry and Biophysics, 1969Purified chloroplast preparations from spinach incorporate [3H]-dTTP1 into an acid-insoluble product. The incorporation reaction is highly dependent on all four deoxynucleoside triphosphates, Mg2+ and a sulphydryl reagent, and is further stimulated by KCl.
Donald Spencer, Paul R. Whitfeld
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A Chloroplast DNA Study of the Agavaceae
Systematic Botany, 1995A chloroplast DNA restriction site analysis of 40 taxa in the Agavaceae s.l. was undertaken to test the accuracy of several current systems of classification. In Cronquist's system, the Agavaceae contain 18 genera of fibrous-leaved, more or less woody plants such as Yucca, Hes- peraloe, Agave, Manfreda, Polianthes, Dracaena, Sansevieria, Nolina ...
David J. Bogler, Beryl B. Simpson
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Miniprep for Chloroplast DNA Isolation
Microchemical Journal, 1993Abstract This paper describes a procedure for the isolation and purification of chloroplastic DNA (cpDNA) designed for undergraduate research projects. It is a modification of the procedures from Doyle and Doyle ( Phytochem. Bull. , 1987, 19 , 11-15), Marmur ( Mol. Biol.
E.P. Oharamays, J.C. Capwell
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Mapping the Chloroplast DNA of Vicia faba
Current Genetics, 1983A complete clone bank representing the chloroplast DNA from Vicia faba has been constructed. A total of 15 fragments (10 Pst1, 1 Pst1-EcoR1 and 4 Sal1 fragments) were inserted into the vector pBR322 and transformed into the E. coli strain HB101. The cloned fragments were used as the main tools in constructing the physical map of Vicia faba for the ...
Kenton Ko +2 more
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