Results 121 to 130 of about 12,852,402 (297)

Atomic Layer Deposition in Transistors and Monolithic 3D Integration

Advanced Functional Materials, EarlyView.
Transistors are fundamental building blocks of modern electronics. This review summarizes recent progress in atomic layer deposition (ALD) for the synthesis of two‐dimensional (2D) metal oxides and transition‐metal dichalcogenides (TMDCs), with particular emphasis on their enabling role in monolithic three‐dimensional (M3D) integration for next ...
Yue Liu, Taoyu Zou, Cheng‐Yan Xu, Liang Zhen, Yang Li, Yong‐Young Noh   +5 more
wiley   +1 more source

Codon Optimization of Stem Cell Factor (SCF) gene and evaluation of this gene expression using vector pBudCE4.1 in Chinese Hamster Ovary cells (CHO) [PDF]

Journal of Fasa University of Medical Sciences, 2017
Background & Objective: Stem cell factor (SCF) is a blood cytokine which can play a significant role in the differentiation of blood precursor cells, survival, proliferation and differentiation of mast cells, and it can also increase the proliferation ...
ehsan heidari, maryam peymai, ali kazemi, kamran Ghaedi   +3 more
doaj   +1 more source

Optimization of heterologous protein production in Chinese hamster ovary cells under overexpression of spliced form of human X-box binding protein [PDF]

, 2014
Background: The optimization of protein production is a complex and challenging problem in biotechnology. Different techniques for transcription, translation engineering and the optimization of cell culture conditions have been used to improve protein ...
Brígido, Marcelo de Macedo, Gulis, Galina, Maranhão, Andrea Queiroz, Simi, Kelly Cristina Rodrigues, Toledo, Renata Rodrigues de   +4 more
core   +2 more sources

Regionally Localized Electrolyte Engineering via UV Crosslinking for Stable Ether–Carbonate Lithium Metal Batteries

Advanced Functional Materials, EarlyView.
This work introduces a regionally localized electrolyte (RLE) that spatially separates ether‐ and carbonate‐based functions to stabilize both Li metal and high‐voltage cathodes. An immobilized ether‐rich layer directs Li+ transport, activates LiNO3 locally, and forms a uniform LiF‐rich SEI, enabling lower overpotential, uniform deposition, and long ...
Eunbin Lim, Jeanie Pearl Dizon Suba, Jaegu Cho, Sukeun Yoon, Kuk Young Cho   +4 more
wiley   +1 more source

Transient transfection induces different intracellular calcium signaling in CHO K1 versus HEK 293 cells [PDF]

, 2018
For the controlled production of recombinant proteinsin mammalian cells by transient transfection, it maybe desirable not only to manipulate, but also todiagnose the expression success early.
Connor, J., Preuss, A., Vogel, H.
core  

Serine one-carbon catabolism with formate overflow [PDF]

, 2016
Serine catabolism to glycine and a one-carbon unit has been linked to the anabolic requirements of proliferating mammalian cells. However, genome-scale modeling predicts a catabolic role with one-carbon release as formate. We experimentally prove that in
Athineos, Dimitris, Blyth, Karen, Gottlieb, Eyal, Kamphorst, Jurre J., Labuschagne, Christiaan Fred, Mackay, Gillian M., Maddocks, Oliver, Meiser, Johannes, Tumanov, Sergey, Van Den Broek, Niels, Vazquez, Alexei, Vousden, Karen   +11 more
core   +1 more source

X‐Functionality–Driven Photocatalytic Hydrogen Evolution in 2D 4‐X‐PEA2SnI4 Perovskites

Advanced Functional Materials, EarlyView.
We report a water‐based synthesis of 2D 4‐X‐PEA2SnI4 perovskite microcrystals with prominent photocatalytic (PC) activity for H2 production. The synergy between organic functionalization and HI‐derived iodide scavenges holes suppress octahedral distortion, and favor electron accumulation, enabling a PC H2 evolution ∼20 µmol·g−1 and long‐term stability ...
Taeyeon Kim, Gayoung Seo, Seong Yeon Park, Juwon Jang, Yunho Ahn, Samiksha Mukesh Jain, Samrat Das Adhikari, Harumi Correa‐Leiva, Ignacio Utreras‐Asenjo, Oscar A. Douglas‐Gallardo, Carina Pareja‐Rivera, Kayoung Cho, Byeongsung Kim, Jhonatan Rodriguez‐Pereira, Eunseo Choi, JaeHong Park, Jin Ho Bang, Danilo H. Jara‐Quinteros, Sixto Giménez, Seog Joon Yoon, Andrés F. Gualdrón‐Reyes, Iván Mora‐Seró   +21 more
wiley   +1 more source

High throughput, efficacious gene editing & genome surveillance in Chinese hamster ovary cells.

PLoS ONE, 2019
Chinese hamster ovary (CHO) cells are a common tool utilized in bioproduction and directed genome engineering of CHO cells is of great interest to enhance recombinant cell lines.
S C Huhn, Y Ou, A Kumar, R Liu, Z Du
doaj   +1 more source

Multiple glycerol shocks increase the calcium phosphate transfection of non-synchronized CHO cells [PDF]

, 2018
The exposure of CHO DG44 cells to an osmotic shock, after DNA uptake, results in a cellular volume decrease of approx. 55%. Repetitive osmotic shocks targeted different sub-populations of cells as was demonstrated using two different fluorescent reporter
Bertschinger, Martin, Grosjean, Frederic, Hacker, David, Wurm, Florian   +3 more
core  

Evidence for the role of proteoglycans in cation-mediated gene transfer [PDF]

, 1996
We report evidence that gene complexes, consisting of polycations and plasmid DNA enter cells via binding to membrane-associated proteoglycans. Treatment of HeLa cells with sodium chlorate, a potent inhibitor of proteoglycan sulfation, reduced luciferase
Baldeschwieler, John D., Mislick, Kimberly A.   +1 more
core   +1 more source