Results 151 to 160 of about 11,449,307 (399)

Synthetic Strategy for mRNA Encapsulation and Gene Delivery with Nanoscale Metal‐Organic Frameworks

Advanced Functional Materials, EarlyView.
This research utilizes the ZIF‐8 for the encapsulation and intracellular delivery of nucleic acids, specifically mRNA, for applications in gene delivery. Integrating PEI addresses the issue of mRNA leakage from ZIF‐8, resulting in the delivery and expression of green fluorescent protein (GFP) in vitro and firefly luciferase in vivo.
Harrison Douglas Lawson, Huy Hoang Nguyen, Keng‐Jung Lee, Nattarat Wongsuwan, Ayesha Tupe, Mengrou Lu, Mariah Lynn Arral, Anne Behre, Zihan Ling, Kathryn Ann Whitehead, Adam Walter Feinberg, Xi Ren, Si‐Yang Zheng   +12 more
wiley   +1 more source

An Attempt to Add Biological Functions by Genetic Engineering in Order to Produce High-Performance Bioreactor Cells for Hybrid Artificial Liver: Transfection of Glutamine Synthetase into Chinese Hamster Ovary (CHO) Cell

Cell Transplantation, 1997
In the course of immortalization, hepatocyte cell lines lose their original differentiated functions, such as ammonia removal and urea formation, drug metabolism, serum protein synthesis, etc. (Enosawa et al., Cell Transplant. 5:S39-S40; 1996).
Shin Enosawa, Seiichi Suzuki, Masayuki Fujino, Hiroshi Amemiya, Takeshi Omasa, Shigeo Urayama, Naoko Tanimura, Kenichi Suga   +7 more
doaj   +1 more source

Canine and Feline Parvoviruses Can Use Human or Feline Transferrin Receptors to Bind, Enter, and Infect Cells [PDF]

, 2001
Canine parvovirus (CPV) enters and infects cells by a dynamin-dependent, clathrin-mediated endocytic pathway, and viral capsids colocalize with transferrin in perinuclear vesicles of cells shortly after entry (J. S. L. Parker and C. R. Parrish, J. Virol.
Murphy, William J., O\u27Brien, Stephen J., Parker, John S. L., Parrish, Colin R., Wang, Dai   +4 more
core   +3 more sources

Comprehensive Si Anode Design for Sulfide‐Based all‐Solid‐State Batteries: Insights into Si‐Electrolyte Synergy for Mitigating Contact Loss

Advanced Functional Materials, EarlyView.
Silicon anodes in sulfide‐based all‐solid‐state batteries suffer from interfacial instability caused by volumetric fluctuations and contact degradation, restricting their practical implementation despite high capacity. This study presents design strategies that leverage controlled active material expansion and an elastic recoverable anolyte to form an ...
Youngjin Song, Sungjin Cho, Suhwan Kim, Youyeong Shin, Ikcheon Na, Jongwoo Lim, Yong Min Lee, Soojin Park   +7 more
wiley   +1 more source

Site-Specific Incorporation of Unnatural Amino Acids into Receptors Expressed in Mammalian Cells [PDF]

, 2003
We describe an approach to achieve unnatural amino acid incorporation into channels and receptors expressed in mammalian cells. We show that microelectroporation provides a general method to deliver DNA, mRNA, and tRNA simultaneously.
Dougherty, Dennis A, Lester, Henry A, Monahan, Sarah L   +2 more
core   +1 more source

H3K4 mono- and di-methyltransferase MLL4 is required for enhancer activation during cell differentiation [PDF]

arXiv, 2013
Enhancers play a central role in cell-type-specific gene expression and are marked by H3K4me1/2. Active enhancers are further marked by H3K27ac. However, the methyltransferases responsible for H3K4me1/2 on enhancers remain elusive. Furthermore, how these enzymes function on enhancers to regulate cell-type-specific gene expression is unclear.
arxiv  

Harnessing Interfacial Cl− Ions for Concurrent Formate Production at Industrial Level via CO2 Reduction and Methanol Oxidation

Advanced Functional Materials, EarlyView.
The replacement of the anodic OER in CO2RR with energy‐saving methanol oxidation reaction and the creation of a favorable microenvironment through interfacial Cl− ions enable concurrent formate production at industrial‐level current densities. Abstract The efficient electrocatalytic conversion of CO2 to formate is often impeded by the high energy ...
Yiqun Chen, Yan Zhang, Zhen Li, Biao Feng, Mengting Li, Qiang Wu, Zheng Hu, Lawrence Yoon Suk Lee   +7 more
wiley   +1 more source

All‐Material Crosslinked Solid Polymer Electrolytes for High‐Performance and Flexible Lithium Metal Battery

Advanced Functional Materials, EarlyView.
A novel solid polymer electrolyte (SPE) to overcome interface instability in high‐energy‐density lithium metal batteries has been developed. By enhancing ionic conductivity, mechanical elasticity, and adhesion strength through all‐material UV‐crosslinking, the SPE ensures uniform lithium‐ion flow and stable performance—even after bending or cutting ...
Sung Yeon Bae, Seoyeon Kim, Young Ho Yoo, Jin‐Seo Kim, Jieun Lee, Jinhan Cho, Bongjun Yeom, Jeong Gon Son   +7 more
wiley   +1 more source

Chromosomal instability drives convergent and divergent evolution toward advantageous inherited traits in mammalian CHO bioproduction lineages

iScience, 2022
Summary: Genetic instability of Chinese hamster ovary (CHO) cells is implicated in production inconsistency through poorly defined mechanisms. Using a multi-omics approach, we analyzed the variations of CHO lineages derived from CHO-K1 cells. We identify
Steve Huhn, Meiping Chang, Amit Kumar, Ren Liu, Bo Jiang, Michael Betenbaugh, Henry Lin, Gregg Nyberg, Zhimei Du   +8 more
doaj  

EFP1 is an ER stress-induced glycoprotein which interacts with the pro-apoptotic protein Par-4 [PDF]

, 2009
We have isolated the rat ortholog of EFP1 (EF-hand binding protein 1) as a novel interaction partner of the pro-apoptotic protein Par-4 (prostate apoptosis response-4).
Appel, Sarah, Boosen, Meike, Koplin, Ansgar, Maertens, Barbara, Preuss, Ute, Vetterkind, Susanne   +5 more
core   +1 more source