Hyperosmolarity enhances transient recombinant protein yield in Chinese hamster ovary cells [PDF]
, 2018 The effect of hyperosmolarity on transient recombinant protein production in Chinese hamster ovary (CHO) cells was investigated. Addition of 90mM NaCl to the production medium ProCHO5 increased the volumetric yield of recombinant antibody up to 4-fold ...
Baldi, Lucia +4 more
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Electrospinning and Nanofiber Technology: Fundamentals, Innovations, and Applications
Advanced Materials, EarlyView.This review explores electrospinning fundamentals, methods for synthesizing polymer, metal oxide, carbon, and composite nanofibers, and advancements in fiber architectures like porous, core–shell, and aligned structures. It highlights applications in functional membranes, sensors, energy systems, and catalyst design while addressing future ...
Yujang Cho +5 more
wiley +1 more source
The Role of Fatty Acid Synthase in the Vascular Smooth Muscle Cell to Foam Cell Transition
CellsVascular smooth muscle cells (VSMCs), in their contractile and differentiated state, are fundamental for maintaining vascular function. Upon exposure to cholesterol (CHO), VSMCs undergo dedifferentiation, adopting characteristics of foam cells—lipid ...
Bethany J. Bogan +9 more
doaj +1 more source
Advanced Materials, EarlyView.Cancer metastasis begins with intravasation, a complex process involving cancer‐endothelial interactions. INVADE (Intravasation‐on‐µDevice), a biomimetic microfluidic platform enables high‐throughput analysis of intravasation under controlled conditions.
Fengtao Jiang +12 more
wiley +1 more source
CIRP Expression on Growth and Productivity of CHO Cells [PDF]
, 2006 Mammalian cell culture is typically operated at the physiological temperature of 37°C. Low temperature cell culture at 30-33°C, in particular for CHO cells, increased the specific productivity of many recombinant proteins amongst many other benefits ...
Tan, Hong-Kiat +2 more
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Evidence for the presence of a receptor for the cytolethal distending toxin (CLDT) of Campylobacter jejuni on CHO and HeLa cell membranes and development of a receptor-based enzyme-linked immunosorbent assay for detection of CLDT [PDF]
, 1993 P Bag
openalex +1 more source
Influence of cell culture media and feed supplements on cell metabolism and quality of IgG produced in CHO-K1, CHO-S, and CHO-DG44 [PDF]
BMC Proceedings, 2015 Andreas Castan +7 more
openaire +2 more sources
Advanced Materials, EarlyView.This review gives a summary on the representative bioinspired single‐atomic‐site catalysts (SACs) and their applications in heterogeneous electrocatalysis and photocatalysis. The fundamentals of bioinspired design strategies are systematically discussed in the context of the first shell coordination, the second/long‐range coordination, and the outer ...
Ying Wang +4 more
wiley +1 more source
Electronic Journal of BiotechnologyBackground: Chinese hamster ovarian (CHO) cells are widely employed in biotechnology for the production of recombinant proteins. Extending the life span of CHO cells and inhibiting the loss of producing cell population through the inhibition of apoptosis
Sara Kafi +4 more
doaj
Retargeting of pre-set regions on chromosome for high gene expression in mammalian cells [PDF]
, 2005 We have developed a system to hunt and reuse special gene integration sites that allow for high and stable gene expression. A vector, named pRGFP8, was constructed. The plasmid pRGFP8 contains a reporter gene, gfp2 and two extraneous DNA fragments.
Chang, Christine +5 more
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