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Chromosome stability in CHO cells
Somatic Cell Genetics, 1977The established cell line derived many years ago from Chinese hamster ovary (CHO cells) has been studied for the extent of chromosomal variation. Because this cell line is used extensively for genetic studies, the contribution of chromosome variability to genetic variability has also been examined. The quasidiploid CHO cells were found to have a banded
R G, Worton, C C, Ho, C, Duff
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Metabolic responses of CHO cells to limitation of key amino acids
Biotechnology and Bioengineering, 2014Nuno Carinhas +2 more
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Dynamic model of CHO cell metabolism
Metabolic Engineering, 2011Fed-batch cultures are extensively used for the production of therapeutic proteins. However, process optimization is hampered by lack of quantitative models of mammalian cellular metabolism in these cultures. This paper presents a new kinetic model of CHO cell metabolism and a novel framework for simulating the dynamics of metabolic and biosynthetic ...
Ryan P, Nolan, Kyongbum, Lee
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Misonidazole-glutathione conjugates in CHO cells
International Journal of Radiation Oncology*Biology*Physics, 1984Misonidazole, after reduction to the hydroxylamine derivative, reacts with glutathione (GSH) under physiological conditions. The reaction product has been identified as a mixture of two isomeric conjugates. When water soluble extracts of CHO cells exposed to misonidazole under hypoxic conditions are subjected to HPLC analysis, misonidazole derivatives,
A J, Varghese, G F, Whitmore
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Novel Genetically Engineered CHO Cells
Genetic Engineering & Biotechnology News, 2016Joaquina Mascarenhas +7 more
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Dynamic model for CHO cell engineering
Journal of Biotechnology, 2012Industrial CHO cell fed-batch processes have progressed significantly over the past decade, with recombinant protein titer consistently reaching the gram per liter level. Such improvements have largely resulted from separate advances in process and cell line development.
Ryan P, Nolan, Kyongbum, Lee
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Synthetic promoters for CHO cell engineering
Biotechnology and Bioengineering, 2014ABSTRACTWe describe for the first time the creation of a library of 140 synthetic promoters specifically designed to regulate the expression of recombinant genes in CHO cells. Initially, 10 common viral promoter sequences known to be active in CHO cells were analyzed using bioinformatic sequence analysis programs to determine the identity and relative ...
Adam J, Brown +3 more
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CRISPR‐interceded CHO cell line development approaches
Biotechnology and Bioengineering, 2023AbstractFor industrial production of recombinant protein biopharmaceuticals, Chinese hamster ovary (CHO) cells represent the most widely adopted host cell system, owing to their capacity to produce high‐quality biologics with human‐like posttranslational modifications.
Shahin Amiri +7 more
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Selenalysine utilization by CHO cells.
Physiological chemistry and physics and medical NMR, 1987CHO cells allowed to grow in a medium containing selenalysine can utilize it for protein synthesis. Selenalysine is incorporated into cell proteins in substitution of lysine: a maximum of 5% of protein lysine can be substituted. Protein lysine substitution by selenalysine can be correlated to the reduced viability of cells grown in its presence.
C. D. Marco +4 more
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Genotoxic effects of ketamine on CHO cells
Archives of Toxicology, 1986Ketamine, a non-barbiturate anaesthetic agent, was studied for its genotoxic potential using the SCE assay. It was genotoxic in the in vitro system at concentrations comparable to the plasma levels achieved during steady state anaesthesia. It had no effects on cellular kinetics in CHO cells.
S G, Adhvaryu +4 more
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