Results 241 to 250 of about 12,852,402 (297)
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cAMP-regulated chloride currents in CHO cells
Biochemical and Biophysical Research Communications, 1992We examined whether elevations in cAMP levels increase membrane chloride permeability in native CHO cells by measuring whole cell chloride currents and efflux of 125I and 36Cl. With 20 microM forskolin, no significant effect was seen on whole cell currents.
A W, Mangel +3 more
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Effects of selenalysine on CHO cells.
Microbiologica, 1986Selenalysine, the lysine isolog with the 4-methylene group substituted by a Selenium atom, inhibits growth rate and plating efficiency of Chinese Hamster Ovary (CHO) cells. It does not affect DNA and RNA synthesis, but inhibits protein synthesis. Cells grown in the presence of selenalysine show a reduced viability and an increased cell volume.
CINI, Chiara +4 more
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CONTINUOUS SUSPENSION CHO CELL CULTURE
1994ABSTRACT A recombinant DNA CHO cell line which produces t-PA was cultivated continuously in suspension (D = 0.5 h−1). The cultivation consisted of four phases with different ammonium chloride concentrations (0,2.5,5 and 7.5 mM) in the feed medium, causing reactor ammonium levels up to 8 mM.
Henrik Albahn Hansen, Claus Emborg
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Activation of transglutaminase during cell cycle in CHO cells
Journal of Cellular Physiology, 1982AbstractTransglutaminase (TGase) activity was measured during cell cycle progression in Chinese hamster ovary (CHO) cells synchronized by release of quiescent cultures and in CHO cells synchronized by mitotic shake off. In cells released from quiescent cultures, a greater than 2‐fold increase in TGase activity occurred within 3 h of stimulation ...
K F, Scott, D H, Russell
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Genotoxicity of goniothalamin in CHO cell line
Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 2004Goniothalamin (GTN) is a styrylpyrrone derivative from Goniothalamus umbrosus and other Annonaceae species. It has been shown to have anti-cancer and apoptosis-inducing properties against various human tumour and animal cell lines. The compound has also been shown to be active in vivo against DMBA-induced rat mammary tumours and was reported as an anti-
Nasir, Umar-Tsafe +4 more
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An 'omics approach towards CHO cell engineering
Biotechnology and Bioengineering, 2013AbstractChinese hamster ovarian cells (CHO) cells have been extensively utilized for industrial production of biopharmaceutical products, such as monoclonal antibodies, human growth hormones, cytokines, and blood‐products. Recent advances in recombinant DNA technology have resulted in the bioengineering of CHO cells that have robust gene amplification ...
Payel, Datta +2 more
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Enhanced CHO Cell-Based Transient Gene Expression with the Epi-CHO Expression System
Molecular Biotechnology, 2010Transient gene expression systems in mammalian cells continue to grow in popularity due to their capacity to produce significant amounts of recombinant protein in a rapid and scalable manner, without the lengthy time periods and resources required for stable cell line development.
Codamo, Joe +4 more
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CHO cell culture media is a complex mixture of nutrients, growth factors, and other supplements. Creative Bioarray aims to provide you with the right CHO cell culture media to support the growth and maintenance of CHO cells. Our media can be divided into two main categories: basal media and supplemental media.
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Effects of thialysine on CHO cells growth.
Microbiologica, 1985Thialysine, the lysine isolog with the 4-methylene group substituted by a sulfur atom, inhibits the growth rate and plating efficiency of Chinese Hamster Ovary (CHO) cells. The inhibition can be reversed by lysine, when added to the culture medium together with thialysine or shortly after; to have a complete reversion a lysine concentration five times ...
M. D. Girolamo +4 more
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Thialysine utilization by thialysine resistant CHO cells.
Physiological chemistry and physics and medical NMR, 1986Thialysine resistant CHO cells utilize thialysine added to the culture medium to a lesser extent than the parental cells. Thialysine is utilized in protein synthesis and it is incorporated into proteins in place of lysine. The parental strain substitutes up to 11% of protein lysine by thialysine, while variant cells substitute a maximum of 5% of ...
A. D. Girolamo +4 more
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