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Genotoxicity of goniothalamin in CHO cell line
Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 2004Goniothalamin (GTN) is a styrylpyrrone derivative from Goniothalamus umbrosus and other Annonaceae species. It has been shown to have anti-cancer and apoptosis-inducing properties against various human tumour and animal cell lines. The compound has also been shown to be active in vivo against DMBA-induced rat mammary tumours and was reported as an anti-
Nasir, Umar-Tsafe +4 more
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Synthetic promoters for CHO cell engineering
Biotechnology and Bioengineering, 2014ABSTRACTWe describe for the first time the creation of a library of 140 synthetic promoters specifically designed to regulate the expression of recombinant genes in CHO cells. Initially, 10 common viral promoter sequences known to be active in CHO cells were analyzed using bioinformatic sequence analysis programs to determine the identity and relative ...
Adam J, Brown +3 more
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Dynamic model of CHO cell metabolism
Metabolic Engineering, 2011Fed-batch cultures are extensively used for the production of therapeutic proteins. However, process optimization is hampered by lack of quantitative models of mammalian cellular metabolism in these cultures. This paper presents a new kinetic model of CHO cell metabolism and a novel framework for simulating the dynamics of metabolic and biosynthetic ...
Ryan P, Nolan, Kyongbum, Lee
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A CHO-cell mutant with a defect in cytokinesis
Somatic Cell Genetics, 1976In a selection procedure designed to enrich for temperature-sensitive mutant cells blocked in mitosis a CHO-cell mutant was isolated which has a defect in cytokinesis as the basis of its temperature-sensitive phenotype. Cultures of the mutant had an abnormally high percentage (ie.
L H, Thompson, P A, Lindl
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Engineering Antibodies on the Surface of CHO Cells
2019While antibody libraries are traditionally screened in phage, bacterial, or yeast display formats, they are produced in large scale for pharmaceutical and commercial use in mammalian cell lines. The simpler organisms used for screening have significantly different folding and glycosylation machinery than mammalian cells; consequently, clones resulting ...
Annalee W, Nguyen +2 more
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Dynamic model for CHO cell engineering
Journal of Biotechnology, 2012Industrial CHO cell fed-batch processes have progressed significantly over the past decade, with recombinant protein titer consistently reaching the gram per liter level. Such improvements have largely resulted from separate advances in process and cell line development.
Ryan P, Nolan, Kyongbum, Lee
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Misonidazole-glutathione conjugates in CHO cells
International Journal of Radiation Oncology*Biology*Physics, 1984Misonidazole, after reduction to the hydroxylamine derivative, reacts with glutathione (GSH) under physiological conditions. The reaction product has been identified as a mixture of two isomeric conjugates. When water soluble extracts of CHO cells exposed to misonidazole under hypoxic conditions are subjected to HPLC analysis, misonidazole derivatives,
A J, Varghese, G F, Whitmore
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Activation of transglutaminase during cell cycle in CHO cells
Journal of Cellular Physiology, 1982AbstractTransglutaminase (TGase) activity was measured during cell cycle progression in Chinese hamster ovary (CHO) cells synchronized by release of quiescent cultures and in CHO cells synchronized by mitotic shake off. In cells released from quiescent cultures, a greater than 2‐fold increase in TGase activity occurred within 3 h of stimulation ...
K F, Scott, D H, Russell
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Effects of selenalysine on CHO cells.
Microbiologica, 1986Selenalysine, the lysine isolog with the 4-methylene group substituted by a Selenium atom, inhibits growth rate and plating efficiency of Chinese Hamster Ovary (CHO) cells. It does not affect DNA and RNA synthesis, but inhibits protein synthesis. Cells grown in the presence of selenalysine show a reduced viability and an increased cell volume.
CINI, Chiara +4 more
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Enhanced CHO Cell-Based Transient Gene Expression with the Epi-CHO Expression System
Molecular Biotechnology, 2010Transient gene expression systems in mammalian cells continue to grow in popularity due to their capacity to produce significant amounts of recombinant protein in a rapid and scalable manner, without the lengthy time periods and resources required for stable cell line development.
Codamo, Joe +4 more
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