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Metabolic engineering of CHO cells to prepare glycoproteins
Emerging Topics in Life Sciences, 2018As a complex and common post-translational modification, N-linked glycosylation affects a recombinant glycoprotein's biological activity and efficacy. For example, the α1,6-fucosylation significantly affects antibody-dependent cellular cytotoxicity and α2,6-sialylation is critical for antibody anti-inflammatory activity.
Qiong, Wang, Michael J, Betenbaugh
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An in-silico study of the regulation of CHO cells glycolysis
Journal of Theoretical Biology, 2014In this work, a kinetic-metabolic model previously developed for CHO cells is used to study glycolysis regulation. The model is assessed for its biological relevance by analyzing its ability to simulate metabolic events induced following a hypoxic perturbation.
Atefeh, Ghorbaniaghdam +2 more
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MicroRNAs as targets for engineering of CHO cell factories
Trends in Biotechnology, 2008MicroRNAs (miRNAs) are strongly implicated in the global regulation of gene expression, and, in this regard, they consequently affect metabolic pathways on every regulatory level in different species. This characteristic makes miRNAs a promising target for cell engineering, and they could have multiple applications in medicine and biotechnology ...
Dethardt, Müller +2 more
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Effects of thialysine on CHO cells growth.
Microbiologica, 1985Thialysine, the lysine isolog with the 4-methylene group substituted by a sulfur atom, inhibits the growth rate and plating efficiency of Chinese Hamster Ovary (CHO) cells. The inhibition can be reversed by lysine, when added to the culture medium together with thialysine or shortly after; to have a complete reversion a lysine concentration five times ...
M. D. Girolamo +4 more
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Effect of Melanin on Radiation Response of CHO Cells
International Journal of Radiation Biology and Related Studies in Physics, Chemistry and Medicine, 1985The effect of the presence of melanin on the response of mammalian cells to ionizing radiation was investigated in a model system utilizing the ability of Chinese hamster ovary cells to incorporate melanin by endocytosis. Cells were incubated in monolayer cultures from 2 to 20 hours with melanin prepared from 'beef eye' or synthesized by air oxidation ...
L E, Hopwood, H M, Swartz, S, Pajak
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Dominance of colchicine resistance in hybrid CHO cells
Somatic Cell Genetics, 1978Intraspecific hybrids of colchicine-sensitive with colchicine-resistant (CHR) Chinese hamster ovary cells were constructed, using six different colchicine-resistant clones from two independent series. In each instance, colchicine resistance was expressed in an incompletely dominant manner.
V, Ling, R M, Baker
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Glycoengineering of CHO Cells to Improve Product Quality
2017Chinese hamster ovary (CHO) cells represent the predominant platform in biopharmaceutical industry for the production of recombinant biotherapeutic proteins, especially glycoproteins. These glycoproteins include oligosaccharide or glycan attachments that represent one of the principal components dictating product quality. Especially important are the N-
Qiong, Wang +3 more
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Behavior of CHO cells on phosphated cellulose membranes
Journal of Biomedical Materials Research, 1998Phosphate groups (negatively charged chemical groups) were grafted onto the surface of cellulose membranes by a reaction between hydroxyl groups of cellulose and phosphorus pentoxide to observe the effect of phosphate groups on cellular behavior. X-ray photoelectron spectroscopy (XPS) was used to determine phosphorylation.
S S, Kim +5 more
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An 'omics approach towards CHO cell engineering
Biotechnology and Bioengineering, 2013AbstractChinese hamster ovarian cells (CHO) cells have been extensively utilized for industrial production of biopharmaceutical products, such as monoclonal antibodies, human growth hormones, cytokines, and blood‐products. Recent advances in recombinant DNA technology have resulted in the bioengineering of CHO cells that have robust gene amplification ...
Payel, Datta +2 more
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The Genetic Consequences of the Thy- Mutation to CHO Cells
1985The behavior and regulation of dNTP pools in somatic cells has long led to speculation that they may play a role in DNA synthesis besides that of simple precursors [2, 20]. While controversy has developed concerning the relationship of dNTP pools to DNA synthesis [18, 19], observations from several laboratories provide convincing evidence that pool ...
M, Meuth, O, Gonçalves, M, Trudel
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