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An 'omics approach towards CHO cell engineering
Biotechnology and Bioengineering, 2013AbstractChinese hamster ovarian cells (CHO) cells have been extensively utilized for industrial production of biopharmaceutical products, such as monoclonal antibodies, human growth hormones, cytokines, and blood‐products. Recent advances in recombinant DNA technology have resulted in the bioengineering of CHO cells that have robust gene amplification ...
Payel, Datta +2 more
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Enhanced CHO Cell-Based Transient Gene Expression with the Epi-CHO Expression System
Molecular Biotechnology, 2010Transient gene expression systems in mammalian cells continue to grow in popularity due to their capacity to produce significant amounts of recombinant protein in a rapid and scalable manner, without the lengthy time periods and resources required for stable cell line development.
Codamo, Joe +4 more
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CHO cell culture media is a complex mixture of nutrients, growth factors, and other supplements. Creative Bioarray aims to provide you with the right CHO cell culture media to support the growth and maintenance of CHO cells. Our media can be divided into two main categories: basal media and supplemental media.
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Effects of thialysine on CHO cells growth.
Microbiologica, 1985Thialysine, the lysine isolog with the 4-methylene group substituted by a sulfur atom, inhibits the growth rate and plating efficiency of Chinese Hamster Ovary (CHO) cells. The inhibition can be reversed by lysine, when added to the culture medium together with thialysine or shortly after; to have a complete reversion a lysine concentration five times ...
M. D. Girolamo +4 more
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Thialysine utilization by thialysine resistant CHO cells.
Physiological chemistry and physics and medical NMR, 1986Thialysine resistant CHO cells utilize thialysine added to the culture medium to a lesser extent than the parental cells. Thialysine is utilized in protein synthesis and it is incorporated into proteins in place of lysine. The parental strain substitutes up to 11% of protein lysine by thialysine, while variant cells substitute a maximum of 5% of ...
A. D. Girolamo +4 more
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ambr® 15 Microbioreactors for CHO Cell Cultivation
2019The ambr 15 has become the industry's standard automated microbioreactor system for mammalian cell culture. It has applications throughout the industry, most commonly for cell line screening and media/feed development. On each ambr 15 workstation, conditions in up to 48 × 15 mL bioreactors can be individually controlled while a liquid handler enables ...
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A Single‐Cell Model for CHO Cellsa
Annals of the New York Academy of Sciences, 1992P, Wu, N G, Ray, M L, Shuler
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Microsome-mediated cytotoxicity to CHO cells
Mutation Research Letters, 1982E L, Tan, R L, Schenley, A W, Hsie
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CHO Cell Antibody Drug Production
Therapeutic antibodies are highly regarded for their high affinity, specificity, low immunogenicity, and ability to target a wide range of biological molecules. Currently, mammalian cells, including Sp2/0, NS0, HEK293, and CHO cells, are primarily used for the production of therapeutic antibodies, with CHO cells accounting for over 70% of the ...openaire +1 more source
Recombinant Protein Production from Stable CHO Cell Pools
The continuous improvement of expression platforms is necessary to respond to the increasing demand for recombinant proteins that are required to carry out structural or functional studies as well as for their characterization as biotherapeutics. While transient gene expression (TGE) in mammalian cells constitutes a rapid and well-established approach,Delafosse, Laurence +9 more
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