Results 191 to 200 of about 110,287 (267)

Spectrophotometric assay for choline acetyltransferase

Analytical Biochemistry, 1972
Abstract A rapid and simple spectrophotometric assay for choline acetyltransferase is described. The method employs 4,4′-dithiodipyridine to measure the coenzyme A produced by the enzymic reaction. The conditions of the assay are described. The results are compared with those obtained by the radiochemical assay of the enzyme.
Li-Pen Chao, Frederick Wolfgram
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Regulation of Choline Acetyltransferase

1989
Publisher Summary This chapter presents the studies related to choline acetyltransferase (ChAT) and cholinergic biology into a more comprehensive neurobiological context. The recent results of biochemical, immunological, molecular biological, arid immunocytochemical studies of ChAT and its regulation are discussed.
J E Vaughn, P M Salvaterra
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Choline acetyltransferase in corneal epithelium

Experimental Eye Research, 1973
Abstract The activity of choline acetyltransferase (EC 2.3.1.6.), the enzyme involved in the synthesis of acetylcholine, was measured by radiochemical assay in homogenates of epithelial cells scraped or cultured from rabbit cornea. The method involves the use of 1- 14 C acetyl-CoA and choline as substrates, resulting in the production of 1- 14 C ...
M.C. Gnädinger, R. Markstein, R. Heimann   +2 more
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ACETYLCHOLINE AND CHOLINE ACETYLTRANSFERASE

Neurochemistry International, 1980
The thermodynamics and kinetics of the alcoholysis of thiolesters are discussed and related to choline acetyltransferase. The conformation and rotational barriers of acetylcholine are described as is the specific inability of choline to induce depolarization. Evidence is presented for the involvement of imidazole and the non-involvement of thiol groups
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Measurement and activation of choline acetyltransferase

Biochemical Pharmacology, 1975
Abstract A method for the radiometric assay of choline acetyltransferase, depending for the separation of the product [1- 14 C]acetylcholine, from the substrate, [1- 14 C]acetyl-CoA, on elution through an anion exchange resin has been modified so as to increase its sensitivity, reduce the blanks and improve the accuracy.
S. P. Mann, Catherine Hebb, Judith Mead
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Choline acetyltransferase in the equine spleen

General Pharmacology: The Vascular System, 1979
Abstract 1. 1. Choline acetyltransferase (ChAc) has been investigated in the spleens of the horse ( Equus cahallus ) and donkey ( Equus asinus ) and found to be present in amounts ranging from 0.34–6.86 μmol of ACh synthesized g −1 hr −1 . Spleens of other ungulates investigated contained much less than this. 2. 2. ACh content of the spleens
L.G. Stephens-Newsham, Heather Banns, S. P. Mann, Catherine Hebb   +3 more
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Choline Acetyltransferase Staining in Brain Sections [PDF]

Cold Spring Harbor Protocols, 2010
INTRODUCTIONThis protocol describes the use of immunocytochemical methods to stain for choline acetyltransferase (ChAT). The avidin-biotin horseradish peroxidase complex (ABC) triple-labeling method used here takes advantage of the extraordinary affinity between avidin and biotin.
Carol Ann Paul, Joanne Berger-Sweeney, Barbara S. Beltz   +2 more
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Choline acetyltransferase and carnitine acetyltransferase in the placenta of the mouse

Comparative Biochemistry and Physiology Part C: Comparative Pharmacology, 1977
Abstract 1. The major 1-14C-acetyl labelled metabolite as identified by high voltage electrophoresis and thin layer chromatography was 1-14C-acetylcarnitine when crude or dialyzed mouse placenta homogenates were incubated in the absence or presence of exogenous 1-carnitine respectively and with 1-14C-acetylcoenzyme A. 2.
Susan K. McCarthy, Frank Welsch
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N‐SUBSTITUTED CHOLINE ANALOGUES AS SUBSTRATES FOR CHOLINE ACETYLTRANSFERASE

Journal of Neurochemistry, 1979
Abstract— Alkyl, phenyl, phenylalkyl and pyridiniumalkyl derivatives of choline were studied as substrates for choline acetyltransferase from bovine brain. When one methyl group of choline was replaced by an ethyl group, pKm (negative logarithm of apparent Km) decreased, whereas Vmax was not significantly changed in comparison with choline.
B. Sörbo, J. Sollenberg, K.-E. Stensiö
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Inhibition of choline acetyltransferase by quaternary ammonium analogues of choline

Biochemical Pharmacology, 1975
Abstract The effects of the choline analogues hemicholinium-3, triethylcholine, tetraethylammonium and acetylcholine on the synthesis of ACh by choline acetyltransferase have been studied in vitro. Hemicholinium-3 is shown to be an inhibitor of choline acetyltransferase with a Ki of 2·5 mM. HC-3, TEA and ACh are competitive with choline.
S. P. Mann, Catherine Hebb
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