Results 231 to 240 of about 129,516 (303)

Choline acetyltransferase in schizophrenia

American Journal of Psychiatry, 1993
To test the hypothesis that schizophrenia involves altered cholinergic tone in the pons, the authors studied post-mortem brain tissue from subjects with schizophrenia.The authors used Western immunoblot to measure the concentration of choline acetyltransferase, an acetylcholine synthesizing enzyme, in the post-mortem brain tissue of 25 schizophrenic ...
C N, Karson, M F, Casanova, J E, Kleinman, W S, Griffin   +3 more
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ACETYLCHOLINE AND CHOLINE ACETYLTRANSFERASE

Neurochemistry International, 1980
The thermodynamics and kinetics of the alcoholysis of thiolesters are discussed and related to choline acetyltransferase. The conformation and rotational barriers of acetylcholine are described as is the specific inability of choline to induce depolarization. Evidence is presented for the involvement of imidazole and the non-involvement of thiol groups
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Differential assay for choline acetyltransferase

Analytical Biochemistry, 1974
Abstract A rapid and sensitive radiochemical assay for choline acetyltransferase (EC 2.3.1.6) is reported. The assay allows for the fact that during incubation of [ 14 C]acetyl-CoA and choline with a cell homogenate, at least one product is formed besides [ 14 C]acetylcholine, which passes an anion exchange column. In contrast to [ 14 C]acetylcholine,
B, Hamprecht, T, Amano
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The phosphorylation of choline acetyltransferase

Neurochemical Research, 1989
Human placental Choline Acetyltransferase (ChAT) has been shown to be phosphorylated in vitro by kinases present in rat brain. Phosphorylation occurs at a single site with the exclusive phosphoamino acid being serine. ChAT phosphorylation was shown to be calcium, and not cyclic nucleotide, dependent and was inhibited by inhibitors of calcium/calmodulin
G, Bruce, L B, Hersh
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Spectrophotometric assay for choline acetyltransferase

Analytical Biochemistry, 1972
Abstract A rapid and simple spectrophotometric assay for choline acetyltransferase is described. The method employs 4,4′-dithiodipyridine to measure the coenzyme A produced by the enzymic reaction. The conditions of the assay are described. The results are compared with those obtained by the radiochemical assay of the enzyme.
L P, Chao, F, Wolfgram
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Immunohistochemistry of Choline Acetyltransferase

1979
Publisher Summary This chapte discusses the problems related to immunohistochemistry of choline acetyltransferase (ChAT)-containing systems in the brain and spinal cord. The synthetic enzyme ChAT is a definitive marker for cholinergic neurons and their processes.
E G, McGeer, P L, McGeer, T, Hattori, V K, Singh   +3 more
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Choline acetyltransferase in corneal epithelium

Experimental Eye Research, 1973
Abstract The activity of choline acetyltransferase (EC 2.3.1.6.), the enzyme involved in the synthesis of acetylcholine, was measured by radiochemical assay in homogenates of epithelial cells scraped or cultured from rabbit cornea. The method involves the use of 1- 14 C acetyl-CoA and choline as substrates, resulting in the production of 1- 14 C ...
M C, Gnädinger, R, Heimann, R, Markstein   +2 more
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Regulation of Choline Acetyltransferase

1989
Publisher Summary This chapter presents the studies related to choline acetyltransferase (ChAT) and cholinergic biology into a more comprehensive neurobiological context. The recent results of biochemical, immunological, molecular biological, arid immunocytochemical studies of ChAT and its regulation are discussed.
P M, Salvaterra, J E, Vaughn
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N‐SUBSTITUTED CHOLINE ANALOGUES AS SUBSTRATES FOR CHOLINE ACETYLTRANSFERASE

Journal of Neurochemistry, 1979
Abstract— Alkyl, phenyl, phenylalkyl and pyridiniumalkyl derivatives of choline were studied as substrates for choline acetyltransferase from bovine brain. When one methyl group of choline was replaced by an ethyl group, pKm (negative logarithm of apparent Km) decreased, whereas Vmax was not significantly changed in comparison with choline.
J, Sollenberg, K E, Stensiö, B, Sörbo
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Choline acetyltransferase in cochlear root neurons

Hearing Research, 1993
Cochlear root neurons are a distinct group of cells located in the auditory nerve root in small rodents. Their transmitter is still unknown. Some of our preparations showed immunoreactivity of somata of cochlear root neurons with both polyclonal and monoclonal antibodies against choline acetyltransferase (ChAT) which, despite their very weak ...
W, Yao, D A, Godfrey
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