Resolving interface structure and local internal mechanics of mitotic chromosomes [PDF]
Nature CommunicationsThe interface of chromosomes enables them to interact with the cell environment and is crucial for their mechanical stability during mitosis. Here, we use Atomic Force Microscopy (AFM) to probe the interface and local micromechanics of the highly ...
Andrea Ridolfi +6 more
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Aurora B and Aurora C pools at two chromosomal regions collaboratively maintain chromosome alignment and prevent aneuploidy at the second meiotic division in mammalian oocytes [PDF]
Frontiers in Cell and Developmental BiologyCorrect chromosome segregation is essential to preserve genetic integrity. The two protein kinases, Aurora B and its meiotic homolog Aurora C, regulate attachments between chromosomal kinetochores and microtubules, thereby contributing to the accuracy of
Anna Kouznetsova +5 more
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Chiasmata and the kinetochore component Dam1 are crucial for elimination of erroneous chromosome attachments and centromere oscillation at meiosis I [PDF]
Open Biology, 2021 Establishment of proper chromosome attachments to the spindle requires elimination of erroneous attachments, but the mechanism of this process is not fully understood.
Misuzu Wakiya +7 more
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eLife, 2022 Chromosome segregation requires both the separation of sister chromatids and the sustained condensation of chromatids during anaphase. In yeast cells, cohesin is not only required for sister chromatid cohesion but also plays a major role determining the ...
Jonay Garcia-Luis +4 more
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Micronucleus Assay: An Early Diagnostic Tool to Assess Genotoxic Changes in Patients with Tobacco Use, Oral Leukoplakia and Oral Submucous Fibrosis [PDF]
Journal of Clinical and Diagnostic Research, 2017 Introduction: Micronuclei (MNi) are acentric chromatid or chromosome fragments produced via genetic damage through genotoxic agents contained in tobacco and betel nut.
Moulshree Kohli +4 more
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Shugoshin1 may play important roles in separation of homologous chromosomes and sister chromatids during mouse oocyte meiosis. [PDF]
PLoS ONE, 2008 BACKGROUND: Homologous chromosomes separate in meiosis I and sister chromatids separate in meiosis II, generating haploid gametes. To address the question why sister chromatids do not separate in meiosis I, we explored the roles of Shogoshin1 (Sgo1) in ...
Shen Yin +9 more
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A multi-step pathway for the establishment of sister chromatid cohesion. [PDF]
PLoS Genetics, 2007 The cohesion of sister chromatids is mediated by cohesin, a protein complex containing members of the structural maintenance of chromosome (Smc) family. How cohesins tether sister chromatids is not yet understood.
Mark Milutinovich +4 more
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Investigating the Interplay between Sister Chromatid Cohesion and Homolog Pairing in Drosophila Nuclei. [PDF]
PLoS Genetics, 2016 Following DNA replication, sister chromatids must stay connected for the remainder of the cell cycle in order to ensure accurate segregation in the subsequent cell division.
T Niroshini Senaratne +3 more
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Single-sister labeling in the C. elegans germline using the nucleotide analog EdU
STAR Protocols, 2022 Summary: Reciprocal exchanges between genetically identical sister chromatids (sister chromatid exchanges or SCEs) have been challenging to study. Here, we describe a protocol that utilizes a pulse/chase of the thymidine analog 5-ethyl-3′-deoxyuridine ...
David E. Almanzar +2 more
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The Interplay of Cohesin and the Replisome at Processive and Stressed DNA Replication Forks
Cells, 2021 The cohesin complex facilitates faithful chromosome segregation by pairing the sister chromatids after DNA replication until mitosis. In addition, cohesin contributes to proficient and error-free DNA replication.
Janne J. M. van Schie, Job de Lange
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