Results 81 to 90 of about 991,372 (400)

Chromatin and Epigenetics [PDF]

Plant Physiology, 2015
Wrapping DNA around protein beads, to form so-called nucleosomes, is an ingenious invention of nature allowing the condensation of an enormous amount of genomic information into a tiny package.
Doris Wagner, Anna Amtmann, Hong Ma
openaire   +3 more sources

Purification tags markedly affect self‐aggregation of CPEB3

FEBS Letters, EarlyView.
Although recombinant proteins are used to study protein aggregation in vitro, uncleaved tags can interfere with accurate interpretation. Our findings demonstrate that His₆‐GFP and His₁₂ tags significantly affect liquid droplet and amyloid fibril formation in the intrinsically disordered region (IDR) of mouse cytoplasmic polyadenylation element‐binding ...
Harunobu Saito, Yujin Lee, Motoharu Ueno, Naotaka Sekiyama, Masatomo So, Ayako Furukawa, Kenji Sugase   +6 more
wiley   +1 more source

Insulator-based loops mediate the spreading of H3K27me3 over distant micro-domains repressing euchromatin genes

Genome Biology, 2020
Background Chromosomes are subdivided spatially to delimit long-range interactions into topologically associating domains (TADs). TADs are often flanked by chromatin insulators and transcription units that may participate in such demarcation. Remarkably,
Alexandre Heurteau, Charlène Perrois, David Depierre, Olivier Fosseprez, Jonathan Humbert, Stéphane Schaak, Olivier Cuvier   +6 more
doaj   +1 more source

The dynamics of replication licensing in live Caenorhabditis elegans embryos [PDF]

, 2012
Accurate DNA replication requires proper regulation of replication licensing, which entails loading MCM-2-7 onto replication origins. In this paper, we provide the first comprehensive view of replication licensing in vivo, using video microscopy of ...
Anton Gartner, Ashley Craig, Baldinger, Blow, Blow, Brauchle, Brenner, Cheeseman, Chen, Clarey, Clarey, DePamphilis, Drury, Edgar, Encalada, Evrin, Galy, Gambus, Gillespie, Hadwiger, Harvey, Ilves, J. Julian Blow, Kamath, Kim, Kisielewska, Korzelius, Kuipers, Li, Li, Li, Liu, Matthieu Querenet, McNairn, McNally, Moyer, Oehlmann, Praitis, Prokhorova, Quinn, Randell, Remi Sonneville, Remus, Rothbauer, Rowles, Shelton, Siddiqui, Speck, Sönnichsen, Tada, Timmons, Waga, Wallenfang, Wohlschlegel, Yanagi, Yang, Zhong   +56 more
core   +4 more sources

Circulating histones as clinical biomarkers in critically ill conditions

FEBS Letters, EarlyView.
Circulating histones are emerging as promising biomarkers in critical illness due to their diagnostic, prognostic, and therapeutic potential. Detection methods such as ELISA and mass spectrometry provide reliable approaches for quantifying histone levels in plasma samples.
José Luis García‐Gimenez, Juan Carlos Ruiz‐Rodríguez, Ricard Ferrer, Raquel Durá, Antonio Artigas, Iván Bajaña, David Bolado López de Andujar, Irene Cánovas‐Cervera, Adrián Ceccato, Luis Chiscano‐Camón, Elena Climent‐Martinez, Georgia García Fernández, Gemma Goma, Verónica Monforte, Beatriz Quevedo‐Sánchez, Adolf Ruiz‐Sanmartín, Antonio Sierra‐Rivera, Nieves Carbonell Monleón   +17 more
wiley   +1 more source

Epithelial cells maintain memory of prior infection with Streptococcus pneumoniae through di-methylation of histone H3

Nature Communications
Epithelial cells are the first point of contact for bacteria entering the respiratory tract. Streptococcus pneumoniae is an obligate human pathobiont of the nasal mucosa, carried asymptomatically but also the cause of severe pneumoniae.
Christine Chevalier, Claudia Chica, Justine Matheau, Adrien Pain, Michael G. Connor, Melanie A. Hamon   +5 more
doaj   +1 more source

Single-cell chromatin accessibility reveals principles of regulatory variation

Nature, 2015
Cell-to-cell variation is a universal feature of life that affects a wide range of biological phenomena, from developmental plasticity to tumour heterogeneity.
Jason D. Buenrostro, Beijing Wu, Ulrike Litzenburger, D. Ruff, Michael L. Gonzales, M. Snyder, Howard Y. Chang, W. Greenleaf   +7 more
semanticscholar   +1 more source

Chromatin: a tunable spring at work inside chromosomes

, 2001
This paper focuses on mechanical aspects of chromatin biological functioning. Within a basic geometric modeling of the chromatin assembly, we give for the first time the complete set of elastic constants (twist and bend persistence lengths, stretch ...
A. E. H. Love, A. Leforestier, A. S. Belmont, A. Wolffe, A. Worcel, Annick Lesne, B. Houchmandzadeh, C. Balbi, C. Baumann, C. Bouchiat, C. L. Woodcock, C. S. O’Hern, Eli Ben-Haïm, F. Livolant, G. Janninck, H. Schiessel, I. Russo, J. Bednar, J. Marko, J. Marko, J. Marko, J. O. Thomas, J. T. Finch, J. W. Phillips, J. Widom, J. Widom, J. Yao, J. Zlatanova, Jean-Marc Victor, K. Luger, K. van Holde, K. van Holde, K. van Holde, L. M. Carruthers, L. M. Carruthers, M. Poirier, O. Kratky, P. T. Lowary, R. D. Kamien, R. R. Netz, S. B. Smith, S. H. Leuba, S. H. Leuba, S. H. Leuba, S. Przybyl, T. Odijk, T. R. Strick, V. Katritch, W. Saenger, Y. Cui, Z. Kam   +50 more
core   +1 more source

Mechanisms and kinetic assays of aminoacyl‐tRNA synthetases

FEBS Letters, EarlyView.
Accurate protein synthesis is crucial for life. The key players are aminoacyl‐tRNA synthetases (AARSs), which read the genetic code by pairing cognate amino acids and tRNAs. AARSs establish high amino acid selectivity by employing physicochemical limits in molecular recognition.
Igor Zivkovic, Morana Dulic, Ita Gruic‐Sovulj   +2 more
wiley   +1 more source

A human cell atlas of fetal chromatin accessibility

Science, 2020
The genomics of human development Understanding the trajectory of a developing human requires an understanding of how genes are regulated and expressed. Two papers now present a pooled approach using three levels of combinatorial indexing to examine the ...
Silvia Domcke, Andrew J. Hill, R. Daza, Junyue Cao, D. O’Day, Hannah A. Pliner, K. Aldinger, Dmitry Pokholok, Fan Zhang, J. Milbank, Michael A. Zager, I. Glass, F. Steemers, D. Doherty, C. Trapnell, D. Cusanovich, J. Shendure   +16 more
semanticscholar   +1 more source