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Chromatin Immunoprecipitation for ChIP-chip and ChIP-seq
Methods in Molecular Biology, 2014Bacterial adaptation to given environmental conditions is largely achieved by complex gene regulatory processes. To address the question how and to what extend single transcriptional regulators modulate gene expression, chromatin immunoprecipitation (ChIP) coupled to DNA microarrays (ChIP-chip) or to next-generation sequencing (ChIP-seq) is one of the ...
Susanne Häussler
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Analysis of Telomere Proteins by Chromatin Immunoprecipitation (ChIP)
Methods in Molecular Biology, 2017Telomere chromatin immunoprecipitation (ChIP) is an experimental method used to determine whether proteins are associated with telomere DNA inside the nuclei of cells and tissues. Telomere-associated proteins are first covalently crosslinked to telomere DNA, and then immunoprecipitated using an antibody specific for the protein of interest. This method
Xuyang Feng, Wenbin Ma
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Quantitative chromatin immunoprecipitation (Q-ChIP) applied to Schistosoma mansoni
Molecular and Biochemical Parasitology, 2009The life-cycle of the platyhelminth parasite Schistosoma mansoni is characterized by marked morphological changes between the various stages that are the result of a complex developmental program. In order to study the role of epigenetic mechanisms in regulating this program, and more particularly the role of changes in histone modifications in the ...
Stephanie Caby, R J Pierce
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Chromatin Immunoprecipitation (ChIP)
Cold Spring Harbor Protocols, 2009INTRODUCTIONChromatin immunoprecipitation (ChIP) is an invaluable method for studying interactions between specific proteins or modified forms of proteins and a genomic DNA region. ChIP can be used to determine whether a transcription factor interacts with a candidate target gene and is used with equal frequency to monitor the presence of histones with
Michael F, Carey +2 more
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DNA-Enrichment Microfluidic Chip for Chromatin Immunoprecipitation
Analytical Chemistry, 2009Chromatin immunoprecipitation (ChIP) is a powerful and widely applied technique for detecting association of individual proteins with specific genomic regions; the technique requires several complex steps and is tedious. In this paper, we develop a microbead-packed microfluidic chip which eliminates most of the laborious, time-consuming, and skill ...
Hyun Jik, Oh +7 more
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Chromatin Immunoprecipitation (ChIP) Methodology and Readouts
2009The identification of direct nuclear hormone receptor gene targets provides clues to their contribution to both development and cancer progression. Until recently, the identification of such direct target genes has relied on a combination of expression analysis and in silico searches for consensus binding motifs in gene promoters.
Massie, Charles E, Mills, Ian G
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