Protocol for using heterologous spike-ins to normalize for technical variation in chromatin immunoprecipitation [PDF]
STAR Protocols, 2021 Summary: Quantifying differential genome occupancy by chromatin immunoprecipitation (ChIP) remains challenging due to variation in chromatin fragmentation, immunoprecipitation efficiencies, and intertube variability. In this protocol, we add heterologous
Franziska Greulich +1 more
exaly +4 more sources
Protocol to investigate bivalent histone modification dynamics via chromatin immunoprecipitation followed by re-chromatin immunoprecipitation [PDF]
STAR ProtocolsSummary: Epigenetic modifications play key roles in regulating transcription by altering chromatin structure and accessibility in response to internal cues or external stimuli.
Zhengyao Shao, Hong Qiao
doaj +2 more sources
Chromatin immunoprecipitation assay
BioTechniques, 2004 Association between proteins and DNA is crucial for many vital cellular functions such as gene transcription, DNA replication and recombination, repair, segregation, chromosomal stability, cell cycle progression, and epigenetic silencing. It is important
Partha M. Das +3 more
doaj +3 more sources
Assessment of sera for chromatin-immunoprecipitation [PDF]
BioTechniques, 2008 Chromatin-immunoprecipitation (ChIP) is a powerful technique for mapping the protein-DNA interactions that occur in living cells. The critical technical determinant for successful ChIP is the availability of an appropriate, “ChIP-grade” serum.
Juliette Nguyen +3 more
doaj +3 more sources
An optimized two-step chromatin immunoprecipitation protocol to quantify the associations of two separate proteins and their common target DNA [PDF]
STAR Protocols, 2021 Summary: Sequential chromatin immunoprecipitation (ChIP) is commonly used to investigate DNA-protein and protein-protein interactions to a specific genomic region. However, it can be tricky to achieve a robust and reproducible signal with sequential ChIP.
Lingli He +3 more
doaj +2 more sources
Cell-free chromatin immunoprecipitation to detect molecular pathways in heart transplantation [PDF]
Life Science Alliance, 2023 Cell-free DNA chromatin immunoprecipitation detects molecular pathways and profiles the sources of tissue injury in heart transplant patients. Existing monitoring approaches in heart transplantation lack the sensitivity to provide deep molecular ...
Moon Kyoo Jang +5 more
doaj +2 more sources
Optimized protocols for chromatin immunoprecipitation of exogenously expressed epitope-tagged proteins [PDF]
STAR Protocols, 2023 Summary: Chromatin immunoprecipitation (ChIP) assay is widely used for investigating the interaction between DNA and DNA-binding proteins such as transcription factors, co-factors, or chromatin-associated proteins.
Wentong Fang, Chengheng Liao, Qing Zhang
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Protocol for chromatin immunoprecipitation of histone modifications in frozen adipose tissue [PDF]
STAR ProtocolsSummary: Chromatin immunoprecipitation (ChIP) combined with sequencing has revolutionized our understanding of gene regulation; however, its application to frozen adipose tissue presents unique challenges due to the high levels of lipid content. Here, we
Akin Cayir +4 more
doaj +2 more sources
Protocol for in vivo chromatin immunoprecipitation on purified chromatin isolated from mouse liver nuclei [PDF]
STAR ProtocolsSummary: Chromatin immunoprecipitation (ChIP) is used to investigate genome binding by transcription factors, but it can be problematic. We present a protocol to isolate fixed DNA-protein complexes from mouse liver prior to chromatin shearing.
Lei Li, May G. Akl, Scott B. Widenmaier
doaj +2 more sources
Allele-Specific Chromatin Immunoprecipitation Studies Show Genetic Influence on Chromatin State in Human Genome [PDF]
PLoS Genetics, 2007 Several recent studies have shown a genetic influence on gene expression variation, including variation between the two chromosomes within an individual and variation between individuals at the population level.
Mitsutaka Kadota +2 more
exaly +2 more sources