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Chromatin immunoprecipitation assay
BioTechniques, 2004 Association between proteins and DNA is crucial for many vital cellular functions such as gene transcription, DNA replication and recombination, repair, segregation, chromosomal stability, cell cycle progression, and epigenetic silencing. It is important
Partha M. Das +3 more
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Assessment of sera for chromatin-immunoprecipitation [PDF]
BioTechniques, 2008 Chromatin-immunoprecipitation (ChIP) is a powerful technique for mapping the protein-DNA interactions that occur in living cells. The critical technical determinant for successful ChIP is the availability of an appropriate, “ChIP-grade” serum.
Juliette Nguyen +3 more
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A native chromatin immunoprecipitation (ChIP) protocol for studying histone modifications in strawberry fruits [PDF]
Plant Methods, 2020 Background Covalent modifications of histones and histone variants have great influence on chromatin structure, which is involved in the transcriptional regulation of gene expression.
Xiaorong Huang +4 more
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The Current State of Chromatin Immunoprecipitation (ChIP) from FFPE Tissues [PDF]
International Journal of Molecular Sciences, 2022 Stefano Amatori, Mirco Fanelli
exaly +2 more sources
Chromatin Immunoprecipitation [PDF]
Cold Spring Harbor Protocols, 2020 Chromatin immunoprecipitation, commonly referred to as ChIP, is a powerful technique for the evaluation of in vivo interactions of proteins with specific regions of genomic DNA. Formaldehyde is used in this technique to cross-link proteins to DNA in vivo, followed by the extraction of chromatin from cross-linked cells and tissues.
James, DeCaprio, Thomas O, Kohl
openaire +3 more sources
Critical parameters for efficient sonication and improved chromatin immunoprecipitation of high molecular weight proteins [PDF]
, 2016 Solubilization of cross-linked cells followed by chromatin shearing is essential for successful chromatin immunoprecipitation (ChIP). However, this task, typically accomplished by ultrasound treatment, may often become a pitfall of the process, due to ...
Adams, Peter D. +2 more
core +14 more sources
Chromatin composition is changed by poly(ADP-ribosyl)ation during chromatin immunoprecipitation. [PDF]
PLoS ONE, 2012 Chromatin-immunoprecipitation (ChIP) employs generally a mild formaldehyde cross-linking step, which is followed by isolation of specific protein-DNA complexes and subsequent PCR testing, to analyze DNA-protein interactions.
Sascha Beneke +4 more
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STAR Protocols, 2021 Summary: Conventional chromatin immunoprecipitation (ChIP) includes many steps that need to be optimized. Here, we have described a protocol of fractionation-assisted native ChIP (fanChIP) which combines subfractionation and native ChIP to purify protein/
Ryo Miyamoto, Akihiko Yokoyama
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Iyer Laboratory Solid Tissue Chromatin Immunoprecipitation Protocol [PDF]
, 2017 A detailed protocol on how to perform chromatin immunoprecipitation in solid tissue samples.
Hall, Amelia
core +1 more source
p65 Chromatin Immunoprecipitation Protocol
Bio-Protocol, 2013 Chromatin Immunoprecipitation (ChIP) is an important procedure that allows you to verify if a certain protein is physically located at a regulatory region.
Crissy Dudgeon
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