Assessment of sera for chromatin-immunoprecipitation [PDF]
BioTechniques, 2008 Chromatin-immunoprecipitation (ChIP) is a powerful technique for mapping the protein-DNA interactions that occur in living cells. The critical technical determinant for successful ChIP is the availability of an appropriate, “ChIP-grade” serum.
Juliette Nguyen +3 more
doaj +3 more sources
Protocol for in vivo chromatin immunoprecipitation on purified chromatin isolated from mouse liver nuclei [PDF]
STAR ProtocolsSummary: Chromatin immunoprecipitation (ChIP) is used to investigate genome binding by transcription factors, but it can be problematic. We present a protocol to isolate fixed DNA-protein complexes from mouse liver prior to chromatin shearing.
Lei Li, May G. Akl, Scott B. Widenmaier
doaj +2 more sources
Plant Chromatin Immunoprecipitation
, 2010 Development of multicellular organisms is based on specialized gene expression programs. Because chromatin establishes the environment for transcription, understanding composition and dynamics of chromatin is an important part of developmental biology.
Villar, C., Köhler, C.
openaire +5 more sources
Fast chromatin immunoprecipitation assay [PDF]
Nucleic Acids Research, 2006 Chromatin immunoprecipitation (ChIP) is a widely used method to explore in vivo interactions between proteins and DNA. The ChIP assay takes several days to complete, involves several tube transfers and uses either phenol-chlorophorm or spin columns to purify DNA. The traditional ChIP method becomes a challenge when handling multiple samples.
Nelson, Joel D. +3 more
openaire +3 more sources
A low-input high resolution sequential chromatin immunoprecipitation method captures genome-wide dynamics of bivalent chromatin [PDF]
Epigenetics & ChromatinBackground Bivalent chromatin is an exemplar of epigenetic plasticity. This co-occurrence of active-associated H3K4me3 and inactive-associated H3K27me3 histone modifications on opposite tails of the same nucleosome occurs predominantly at promoters that ...
Janith A. Seneviratne +3 more
doaj +2 more sources
An Optimized Chromatin Immunoprecipitation Protocol for Quantification of Protein-DNA Interactions [PDF]
STAR Protocols, 2020 Summary: Transcription factors are important regulators of cell fate and function. Knowledge about where transcription factors are bound in the genome is crucial for understanding their function.
Wim J. de Jonge +3 more
doaj +2 more sources
A native chromatin immunoprecipitation (ChIP) protocol for studying histone modifications in strawberry fruits [PDF]
Plant Methods, 2020 Background Covalent modifications of histones and histone variants have great influence on chromatin structure, which is involved in the transcriptional regulation of gene expression.
Xiaorong Huang +4 more
doaj +2 more sources
Chromatin RNA Immunoprecipitation (ChRIP)
, 2017 Researchers have recently had a growing interest in understanding the functional role of long noncoding RNAs (lncRNAs) in chromatin organization. Accumulated evidence suggests lncRNAs could act as interphase molecules between chromatin and chromatin remodelers to define the epigenetic code.
Tanmoy, Mondal +2 more
openaire +3 more sources
Chromatin immunoprecipitation v1
, 2017 This protocol can be ued for chromatin immunoprecipitation of RNAPII and associated factors, as well as histones. The settings are given for HeLa cells and should be adapted for other cell types.
Gabriel Sanchez, Rosemary Kiernan
openaire +2 more sources
The Current State of Chromatin Immunoprecipitation (ChIP) from FFPE Tissues [PDF]
International Journal of Molecular Sciences, 2022 Stefano Amatori +2 more
exaly +2 more sources