Results 11 to 20 of about 137,564 (241)
A low-input high resolution sequential chromatin immunoprecipitation method captures genome-wide dynamics of bivalent chromatin [PDF]
Epigenetics & ChromatinBackground Bivalent chromatin is an exemplar of epigenetic plasticity. This co-occurrence of active-associated H3K4me3 and inactive-associated H3K27me3 histone modifications on opposite tails of the same nucleosome occurs predominantly at promoters that ...
Janith A. Seneviratne +3 more
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An Optimized Chromatin Immunoprecipitation Protocol for Quantification of Protein-DNA Interactions [PDF]
STAR Protocols, 2020 Summary: Transcription factors are important regulators of cell fate and function. Knowledge about where transcription factors are bound in the genome is crucial for understanding their function.
Wim J. de Jonge +3 more
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A native chromatin immunoprecipitation (ChIP) protocol for studying histone modifications in strawberry fruits [PDF]
Plant Methods, 2020 Background Covalent modifications of histones and histone variants have great influence on chromatin structure, which is involved in the transcriptional regulation of gene expression.
Xiaorong Huang +4 more
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The Current State of Chromatin Immunoprecipitation (ChIP) from FFPE Tissues [PDF]
International Journal of Molecular Sciences, 2022 Stefano Amatori +2 more
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Chromatin Immunoprecipitation [PDF]
Cold Spring Harbor Protocols, 2020 Chromatin immunoprecipitation, commonly referred to as ChIP, is a powerful technique for the evaluation of in vivo interactions of proteins with specific regions of genomic DNA. Formaldehyde is used in this technique to cross-link proteins to DNA in vivo, followed by the extraction of chromatin from cross-linked cells and tissues.
James, DeCaprio, Thomas O, Kohl
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High-resolution chromatin immunoprecipitation [PDF]
Nature Methods, 2012 Tal Nawy, Nawy Tal
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Chromatin composition is changed by poly(ADP-ribosyl)ation during chromatin immunoprecipitation. [PDF]
PLoS ONE, 2012 Chromatin-immunoprecipitation (ChIP) employs generally a mild formaldehyde cross-linking step, which is followed by isolation of specific protein-DNA complexes and subsequent PCR testing, to analyze DNA-protein interactions.
Sascha Beneke +4 more
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STAR Protocols, 2021 Summary: Conventional chromatin immunoprecipitation (ChIP) includes many steps that need to be optimized. Here, we have described a protocol of fractionation-assisted native ChIP (fanChIP) which combines subfractionation and native ChIP to purify protein/
Ryo Miyamoto, Akihiko Yokoyama
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p65 Chromatin Immunoprecipitation Protocol
Bio-Protocol, 2013 Chromatin Immunoprecipitation (ChIP) is an important procedure that allows you to verify if a certain protein is physically located at a regulatory region.
Crissy Dudgeon
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RNA Chromatin Immunoprecipitation (RNA-ChIP) in Caenorhabditis elegans
Bio-Protocol, 2014 The RNA chromatin immunoprecipitation assay (RNA-ChIP) allows detection and quantification of RNA–protein interactions using in vivo cross-linking with formaldehyde followed by immunoprecipitation of the RNA–protein complexes.
Germano Cecere, Alla Grishok
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