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Chromatin immunoprecipitation assay
BioTechniques, 2004 Association between proteins and DNA is crucial for many vital cellular functions such as gene transcription, DNA replication and recombination, repair, segregation, chromosomal stability, cell cycle progression, and epigenetic silencing. It is important
Partha M. Das +3 more
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Native Chromatin Immunoprecipitation [PDF]
, 2011 Native chromatin immunoprecipitation refers to a method that allows for identification and quantification of DNA that is associated with specific chromatin proteins without altering the structure of these proteins. The method has been used with great success in the past and has some advantages over the more widely used cross-linking chromatin ...
Cosseau, Céline, Grunau, Christoph
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Assessment of sera for chromatin-immunoprecipitation [PDF]
BioTechniques, 2008 Chromatin-immunoprecipitation (ChIP) is a powerful technique for mapping the protein-DNA interactions that occur in living cells. The critical technical determinant for successful ChIP is the availability of an appropriate, “ChIP-grade” serum.
Juliette Nguyen +3 more
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Methods, 2016 Chromatin immunoprecipitation (ChIP) is a valuable method to investigate protein-DNA interactions in vivo. Since its discovery it has been indispensable to identify binding sites and patterns of a variety of DNA-interacting proteins, such as transcription factors and regulators, modified histones, and epigenetic modifiers.
Laura, Wiehle, Achim, Breiling
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Plant Chromatin Immunoprecipitation
, 2010 Development of multicellular organisms is based on specialized gene expression programs. Because chromatin establishes the environment for transcription, understanding composition and dynamics of chromatin is an important part of developmental biology.
Villar, C., Köhler, C.
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Fast chromatin immunoprecipitation assay [PDF]
Nucleic Acids Research, 2006 Chromatin immunoprecipitation (ChIP) is a widely used method to explore in vivo interactions between proteins and DNA. The ChIP assay takes several days to complete, involves several tube transfers and uses either phenol-chlorophorm or spin columns to purify DNA. The traditional ChIP method becomes a challenge when handling multiple samples.
Nelson, Joel D. +3 more
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A native chromatin immunoprecipitation (ChIP) protocol for studying histone modifications in strawberry fruits [PDF]
Plant Methods, 2020 Background Covalent modifications of histones and histone variants have great influence on chromatin structure, which is involved in the transcriptional regulation of gene expression.
Xiaorong Huang +4 more
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Chromatin RNA Immunoprecipitation (ChRIP)
, 2017 Researchers have recently had a growing interest in understanding the functional role of long noncoding RNAs (lncRNAs) in chromatin organization. Accumulated evidence suggests lncRNAs could act as interphase molecules between chromatin and chromatin remodelers to define the epigenetic code.
Tanmoy, Mondal +2 more
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Chromatin immunoprecipitation v1
, 2017 This protocol can be ued for chromatin immunoprecipitation of RNAPII and associated factors, as well as histones. The settings are given for HeLa cells and should be adapted for other cell types.
Gabriel Sanchez, Rosemary Kiernan
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The Current State of Chromatin Immunoprecipitation (ChIP) from FFPE Tissues [PDF]
International Journal of Molecular Sciences, 2022 Stefano Amatori +2 more
exaly +2 more sources