Impact of chromatin structures on DNA processing for genomic analyses. [PDF]
PLoS ONE, 2009 Chromatin has an impact on recombination, repair, replication, and evolution of DNA. Here we report that chromatin structure also affects laboratory DNA manipulation in ways that distort the results of chromatin immunoprecipitation (ChIP) experiments. We
Leonid Teytelman +6 more
doaj +1 more source
Journal of Lipid Research, 2006 Pancreatic β-cell dysfunction is central to the pathogenesis of type 2 diabetes and may involve secretory failure through glucolipotoxity. The relative importance of the transcription factors carbohydrate-responsive element binding protein (ChREBP ...
Gabriela da Silva Xavier +4 more
doaj +1 more source
Chromatin immunoprecipitation: optimization, quantitative analysis and data normalization
Plant Methods, 2007 Background Chromatin remodeling, histone modifications and other chromatin-related processes play a crucial role in gene regulation. A very useful technique to study these processes is chromatin immunoprecipitation (ChIP).
Peterhansel Christoph +5 more
doaj +1 more source
Titration-based normalization of antibody amount improves consistency of ChIP-seq experiments
BMC Genomics, 2023 Chromatin immunoprecipitation (ChIP) is an antibody-based approach that is frequently utilized in chromatin biology and epigenetics. The challenge in experimental variability by unpredictable nature of usable input amounts from samples and undefined ...
Ariel Caride +11 more
doaj +1 more source
Function of the upstream activating factor in chromatin structure organization and transcriptional regulation at the yeast ribosomal DNA [PDF]
, 2010 Chromatin is the template of all processes involved in DNA metabolism in the eukaryotic cell. Accordingly, chromatin is a dynamic structure which changes in its composition and posttranslational modification correlating with the functional state of a ...
Götze, Hannah
core +1 more source
From human monocytes to genome-wide binding sites--a protocol for small amounts of blood: monocyte isolation/ChIP-protocol/library amplification/genome wide computational data analysis. [PDF]
PLoS ONE, 2014 Chromatin immunoprecipitation in combination with a genome-wide analysis via high-throughput sequencing is the state of the art method to gain genome-wide representation of histone modification or transcription factor binding profiles. However, chromatin
Sebastian Weiterer +5 more
doaj +1 more source
Chromatin Immunoprecipitation (ChIP) from Mouse Liver Nuclei
, 2021 Understanding the binding of regulatory proteins to their cognate genomic sites is an important step in deciphering transcriptional networks such as the circadian oscillator.
Ripperger, Jürgen A. +1 more
core +1 more source
Inherent signals in sequencing-based Chromatin-ImmunoPrecipitation control libraries.
PLoS ONE, 2009 BackgroundThe growth of sequencing-based Chromatin Immuno-Precipitation studies call for a more in-depth understanding of the nature of the technology and of the resultant data to reduce false positives and false negatives.
Vinsensius B Vega +3 more
doaj +1 more source
Peripheral lysosomes recruit PLEKHG3 to focal adhesions and restrain protrusion dynamics
FEBS Letters, EarlyView.Proximity‐dependent labeling at the LAMTOR complex revealed the Rho GEF PLEKHG3 as a lysosome‐proximal protein directing the study toward the influence of lysosome positioning on actin dynamics and cell motility. We show that PLEKHG3 colocalizes with lysosomes at focal adhesion sites and observe that forced peripheral dispersion of lysosomes hinders ...
Rainer Ettelt +8 more
wiley +1 more source
Antibodies and conditions used for chromatin immunoprecipitation (ChIP).
, 2023 Antibodies and conditions used for chromatin immunoprecipitation (ChIP).
Karsten Hokamp (42210) +11 more
core +1 more source