Results 41 to 50 of about 202,299 (282)

Trichostatin A induces 5-lipoxygenase promoter activity and mRNA expression via inhibition of histone deacetylase 2 and 3 [PDF]

, 2012
The 5-lipoxygenase (5-LO) is the key enzyme in the formation of leukotrienes. We have previously shown that the histone deacetylase (HDAC) inhibitor trichostatin A (TSA) activates 5-LO transcription via recruitment of Sp1, Sp3 and RNA polymerase II to ...
Grez, Manuel, Katryniok, Careen, Metzner, Julia, Pufahl, Laura, Schnur, Nicole, Sorg, Bernd Lothar, Steinhilber, Dieter   +6 more
core   +1 more source

Glycogen Synthase Kinase (GSK) 3β phosphorylates and protects nuclear myosin 1c from proteasome-mediated degradation to activate rDNA transcription in early G1 cells [PDF]

, 2014
Nuclear myosin 1c (NM1) mediates RNA polymerase I (pol I) transcription activation and cell cycle progression by facilitating PCAF-mediated H3K9 acetylation, but the molecular mechanism by which NM1 is regulated remains unclear.
A Cole, A Hay-Koren, A Sarshad, A Vintermist, A-S Tseng, AA Sérandour, AC O'Sullivan, Aishe A. Sarshad, AM Al-Khouri, Anne Von Euler, Bader Al-Muzzaini, CJ Fiol, D Drygin, D Wu, Douglas Lamont, DW Young, E Cavellán, E Kinoshita, E Sanij, F Mohn, GE Zentner, I Grummt, J Grisouard, J Russell, J Ye, JR Woodgett, JR Woodgett, JR Woodgett, L Meijer, Laura Borgonovo-Brandter, LC Fuentealba, M Benavides, M Liu, M Rosner, M Welcker, MA Munoz, Martin Corcoran, N Fomproix, N Visa, Neus Visa, NG Kim, P Cohen, P de Lanerolle, P Percipalle, P Percipalle, Piergiorgio Percipalle, Raffaella Santoro, RS Jope, S Boulon, T Moss, T Tasaki, T Vincent, V Stambolic, VA Smits, VF Taelman, VV Philimonenko, VW Ding, WJ Ryves, YC Tsai   +58 more
core   +9 more sources

Chromatin immunoprecipitation: optimization, quantitative analysis and data normalization

Plant Methods, 2007
Background Chromatin remodeling, histone modifications and other chromatin-related processes play a crucial role in gene regulation. A very useful technique to study these processes is chromatin immunoprecipitation (ChIP).
Peterhansel Christoph, Horst Ina, Danker Tanja, Offermann Sascha, Haring Max, Stam Maike   +5 more
doaj   +1 more source

Titration-based normalization of antibody amount improves consistency of ChIP-seq experiments

BMC Genomics, 2023
Chromatin immunoprecipitation (ChIP) is an antibody-based approach that is frequently utilized in chromatin biology and epigenetics. The challenge in experimental variability by unpredictable nature of usable input amounts from samples and undefined ...
Ariel Caride, Jin Sung Jang, Geng-Xian Shi, Sam Lenz, Jian Zhong, Kwan Hyun Kim, Mariet Allen, Keith D. Robertson, Gianrico Farrugia, Tamas Ordog, Nilüfer Ertekin-Taner, Jeong-Heon Lee   +11 more
doaj   +1 more source

From human monocytes to genome-wide binding sites--a protocol for small amounts of blood: monocyte isolation/ChIP-protocol/library amplification/genome wide computational data analysis. [PDF]

PLoS ONE, 2014
Chromatin immunoprecipitation in combination with a genome-wide analysis via high-throughput sequencing is the state of the art method to gain genome-wide representation of histone modification or transcription factor binding profiles. However, chromatin
Sebastian Weiterer, Florian Uhle, Sabin Bhuju, Michael Jarek, Markus A Weigand, Marek Bartkuhn   +5 more
doaj   +1 more source

Methods for Scarless, Selection-Free Generation of Human Cells and Allele-Specific Functional Analysis of Disease-Associated SNPs and Variants of Uncertain Significance. [PDF]

, 2017
With the continued emergence of risk loci from Genome-Wide Association studies and variants of uncertain significance identified from patient sequencing, better methods are required to translate these human genetic findings into improvements in public ...
Carvajal-Carmona, Luis G, Coggins, Nicole B, O'Geen, Henriette, Segal, David J, Stultz, Jacob   +4 more
core   +4 more sources

ChREBP binding to fatty acid synthase and L-type pyruvate kinase genes is stimulated by glucose in pancreatic β-cells

Journal of Lipid Research, 2006
Pancreatic β-cell dysfunction is central to the pathogenesis of type 2 diabetes and may involve secretory failure through glucolipotoxity. The relative importance of the transcription factors carbohydrate-responsive element binding protein (ChREBP ...
Gabriela da Silva Xavier, Guy A. Rutter, Frédérique Diraison, Chrysovalantis Andreolas, Isabelle Leclerc   +4 more
doaj   +1 more source

Chromatin Preparation and Chromatin Immuno-precipitation from Drosophila Embryos

, 2016
This protocol provides specific details on how to perform Chromatin immunoprecipitation (ChIP) from Drosophila embryos. ChIP allows the matching of proteins or histone modifications to specific genomic regions.
Iovino, N., Latrielle, D., Löser, E.
core   +1 more source

Human THO–Sin3A interaction reveals new mechanisms to prevent R-loops that cause genome instability [PDF]

, 2017
R-loops, formed by co-transcriptional DNA–RNA hybrids and a displaced DNA single strand (ssDNA), fulfill certain positive regulatory roles but are also a source of genomic instability.
Aguilera López, Andrés, Bayona Feliu, Aleix, Luna Varo, Rosa María, Pérez Calero, Carmen, Salas Armenteros, Irene, Tumini, Emanuela   +5 more
core   +1 more source

Convergence of Wnt signalling on the HNF4a-driven transcription in controlling liver zonation [PDF]

, 2009
BACKGROUND & AIMS: In each hepatocyte, the specific repertoire of gene expression is influenced by its exact location along the portocentrovenular axis of the hepatic lobule and provides a reason for the liver functions compartmentalization defined ...
Alonzi, T, Amicone, Laura, Cicchini, Carla, Colletti, M, Conigliaro, Alice, Pasquini, E, Santangelo, L, Tripodi, Marco   +7 more
core   +1 more source