Results 61 to 70 of about 202,299 (282)

Inherent signals in sequencing-based Chromatin-ImmunoPrecipitation control libraries.

PLoS ONE, 2009
BackgroundThe growth of sequencing-based Chromatin Immuno-Precipitation studies call for a more in-depth understanding of the nature of the technology and of the resultant data to reduce false positives and false negatives.
Vinsensius B Vega, Edwin Cheung, Nallasivam Palanisamy, Wing-Kin Sung   +3 more
doaj   +1 more source

Localized inhibition of protein phosphatase 1 by NUAK1 promotes spliceosome activity and reveals a MYC-sensitive feedback control of transcription. [PDF]

, 2020
Deregulated expression of MYC induces a dependence on the NUAK1 kinase, but the molecular mechanisms underlying this dependence have not been fully clarified.
Ade, C.P., Baluapuri, A., Chang, Y.C., Cossa, G., Eilers, M., Girard, C., Kempa, S., Kumar, A., Kuster, B., Lührmann, R., Mastrobuoni, G., Mumberg, D., Prinz, F., Roeschert, I., Schülein-Völk, C., Silveira Vidal, R., Walz, S., Wolf, E., Wortmann, L.   +18 more
core   +1 more source

Loss of IGF‐1R impairs DNA‐PKcs recruitment to chromatin leading to defective end‐joining

Molecular Oncology, EarlyView.
IGF‐1R promotes radioresistance by facilitating DNA‐PKcs recruitment to chromatin, enabling non‐homologous end‐joining (NHEJ) repair of double‐strand breaks. Inhibition or loss of IGF‐1R disrupts this recruitment to damage sites, driving compensatory reliance on microhomology‐mediated end‐joining (MMEJ) repair.
Matthew O. Ellis, Jack V. Mills, Wojciech Niedzwiedz, Valentine M. Macaulay   +3 more
wiley   +1 more source

Protocol: methodology for chromatin immunoprecipitation (ChIP) in Chlamydomonas reinhardtii

Plant Methods, 2011
We report on a detailed chromatin immunoprecipitation (ChIP) protocol for the unicellular green alga Chlamydomonas reinhardtii. The protocol is suitable for the analysis of nucleosome occupancy, histone modifications and transcription factor binding ...
Strenkert Daniela, Schmollinger Stefan, Schroda Michael   +2 more
doaj   +1 more source

Targeted Derepression of the Human Immunodeficiency Virus Type 1 Long Terminal Repeat by Pyrrole-Imidazole Polyamides [PDF]

, 2002
The host factor LSF represses the human immunodeficiency virus type 1 long terminal repeat (LTR) by mediating recruitment of histone deacetylase. We show that pyrrole-imidazole polyamides targeted to the LTR can specifically block LSF binding both in ...
Coull, Jason J., Dervan, Peter B., He, Guocheng, Margolis, David M., Melander, Christian, Rucker, Victor C.   +5 more
core   +1 more source

Multi‐omics and low‐input proteomics profiling reveals dynamic regulation driving pluripotency initiation in early mouse embryos

FEBS Open Bio, EarlyView.
Mouse pre‐implantation development involves a transition from totipotency to pluripotency. Integrating transcriptomics, epigenetic profiling, low‐input proteomics and functional assays, we show that eight‐cell embryos retain residual totipotency features, whereas cytoskeletal remodeling regulated by the ubiquitin‐proteasome system drives progression ...
Wanqiong Li, Xi Xiao, Lingbin Qi, Chanyi Li, Sirui Song, Jiaying Qin, Zhigang Xue, Jinfeng Xue, Huaifang Li   +8 more
wiley   +1 more source

Chromatin Immunoprecipitation (ChIP), Streptavidin and ATP-agarose Mediated Pull-down Analyses

Bio-Protocol, 2013
Epstein-Barr virus (EBV) nuclear antigen 2 (EBNA2) induces expression of both viral and cellular genes in virus infected B cells by mimicking activated Notch receptors (Notch-IC) that mediate transcription activation through binding to the repressing ...
Cheng-Der Liu, Ya-Lin Chen, Yi-Ly Min, Bo Zhao, Chi-Ping Cheng, Myung-Soo Kang, Shu-Jun Chiu, Elliott Kieff, Chih-Wen Peng   +8 more
doaj   +1 more source

Histone modification ChIP-seq on Arabidopsis thaliana Plantlets

Bio-Protocol, 2021
Characterizing the molecular mechanisms regulating gene expression is crucial for understanding the regulatory processes underlying physiological responses to environmental and developmental signals in eukaryotes.
Juan Ramirez-Prado, David Latrasse, Moussa Benhamed   +2 more
doaj   +1 more source

Therapeutic Challenge with a CDK 4/6 Inhibitor Induces an RB-Dependent SMAC-Mediated Apoptotic Response in Non-Small Cell Lung Cancer. [PDF]

, 2018
Purpose: The retinoblastoma tumor suppressor (RB), a key regulator of cell-cycle progression and proliferation, is functionally suppressed in up to 50% of non-small cell lung cancer (NSCLC).
Addya, Sankar, Bhardwaj, Anshul, Birbe, Ruth, Chellappagounder, Thangavel, Den, Robert B., Dicker, Adam P., Ertel, Adam, Ettickan, Boopathi, Haber, Alex, Knudsen, Karen E., Liu, Yi, McNair, Christopher, Perepelyuk, Maryna, Salvino, Joseph M., Shoyele, Sunday   +14 more
core   +1 more source

Identifying transcription factors controlling the basal expression of human MRP4 highlights a substantial role for Sp1

FEBS Open Bio, EarlyView.
The MRP4 transporter exports several drugs and signaling molecules. Here, we identified key promoter elements regulating basal MRP4 expression. Using reporter assays, we defined a conserved region with essential Sp1 and contributory Ets sites, which controlled basal MRP4 expression.
Debora Singer, Angelique Kragl, Katrin Ziems, Juliane Glaubitz, Sophie Grammbauer, Susanne Hildebrandt, Mladen V. Tzvetkov, Gabriele Jedlitschky   +7 more
wiley   +1 more source