A Computational Model of Quantitative Chromatin Immunoprecipitation (ChIP) Analysis
Cancer Informatics, 2008 Chromatin immunoprecipitation (ChIP) analysis is widely used to identify the locations in genomes occupied by transcription factors (TFs). The approach involves chemical cross-linking of DNA with associated proteins, fragmentation of chromatin by ...
Jingping Xie +2 more
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The Current State of Chromatin Immunoprecipitation (ChIP) from FFPE Tissues [PDF]
International Journal of Molecular Sciences, 2022 : Cancer cells accumulate epigenomic aberrations that contribute to cancer initiation and progression by altering both the genomic stability and the expression of genes. The awareness of such alterations could improve our understanding of cancer dynamics
Stefano Amatori +2 more
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A native chromatin immunoprecipitation (ChIP) protocol for studying histone modifications in strawberry fruits [PDF]
Plant Methods, 2020 Background Covalent modifications of histones and histone variants have great influence on chromatin structure, which is involved in the transcriptional regulation of gene expression.
Xiaorong Huang +4 more
doaj +2 more sources
Chromatin Immunoprecipitation (ChIP) to Assess Histone Marks in Auxin-treated Arabidopsis thaliana Inflorescence Tissue [PDF]
Bio-Protocol, 2020 Chromatin immunoprecipitation coupled with quantitative PCR (ChIP-qPCR) or high-throughput sequencing (ChIP-seq) has become the gold standard for the identification of binding sites of DNA binding proteins and the localization of histone modification on ...
Andre Kuhn, Lars Østergaard
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Protocol: methodology for chromatin immunoprecipitation (ChIP) in
Plant Methods, 2011 We report on a detailed chromatin immunoprecipitation (ChIP) protocol for the unicellular green alga Chlamydomonas reinhardtii. The protocol is suitable for the analysis of nucleosome occupancy, histone modifications and transcription factor binding ...
Strenkert Daniela +2 more
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ChIPprimersDB: a public repository of verified qPCR primers for chromatin immunoprecipitation (ChIP) [PDF]
Nucleic Acids Research, 2019 Stefan Kurtenbach +2 more
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Chromatin Immunoprecipitation (ChIP) of Plasmid-Bound Proteins in Xenopus Egg Extracts [PDF]
Methods in Molecular Biology, 2019 David T Long
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Chromatin Immunoprecipitation (ChIP) of Histone Modifications from Saccharomyces cerevisiae. [PDF]
J Vis Exp, 2017 Histone post-translational modifications (PTMs), such as acetylation, methylation and phosphorylation, are dynamically regulated by a series of enzymes that add or remove these marks in response to signals received by the cell.
Jezek M, Jacques A, Jaiswal D, Green EM.
europepmc +2 more sources
STAR Protocols, 2021 Summary: Quantifying differential genome occupancy by chromatin immunoprecipitation (ChIP) remains challenging due to variation in chromatin fragmentation, immunoprecipitation efficiencies, and intertube variability. In this protocol, we add heterologous
Franziska Greulich +3 more
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STAR Protocols, 2021 Summary: Conventional chromatin immunoprecipitation (ChIP) includes many steps that need to be optimized. Here, we have described a protocol of fractionation-assisted native ChIP (fanChIP) which combines subfractionation and native ChIP to purify protein/
Ryo Miyamoto, Akihiko Yokoyama
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