Results 1 to 10 of about 99,245 (113)

A native chromatin immunoprecipitation (ChIP) protocol for studying histone modifications in strawberry fruits. [PDF]

open access: yesPlant Methods, 2020
Background Covalent modifications of histones and histone variants have great influence on chromatin structure, which is involved in the transcriptional regulation of gene expression.
Huang X, Pan Q, Lin Y, Gu T, Li Y.
europepmc   +2 more sources

Chromatin Immunoprecipitation (ChIP) to Assess Histone Marks in Auxin-treated <i>Arabidopsis thaliana</i> Inflorescence Tissue. [PDF]

open access: yesBio Protoc, 2020
Chromatin immunoprecipitation coupled with quantitative PCR (ChIP-qPCR) or high-throughput sequencing (ChIP-seq) has become the gold standard for the identification of binding sites of DNA binding proteins and the localization of histone modification on ...
Kuhn A, Østergaard L.
europepmc   +2 more sources

The Current State of Chromatin Immunoprecipitation (ChIP) from FFPE Tissues [PDF]

open access: yesInternational Journal of Molecular Sciences, 2022
Stefano Amatori   +2 more
exaly   +2 more sources

A Computational Model of Quantitative Chromatin Immunoprecipitation (ChIP) Analysis

open access: yesCancer Informatics, 2008
Chromatin immunoprecipitation (ChIP) analysis is widely used to identify the locations in genomes occupied by transcription factors (TFs). The approach involves chemical cross-linking of DNA with associated proteins, fragmentation of chromatin by ...
Brett Mckinney   +2 more
exaly   +4 more sources

Protocol for using heterologous spike-ins to normalize for technical variation in chromatin immunoprecipitation

open access: yesSTAR Protocols, 2021
Summary: Quantifying differential genome occupancy by chromatin immunoprecipitation (ChIP) remains challenging due to variation in chromatin fragmentation, immunoprecipitation efficiencies, and intertube variability. In this protocol, we add heterologous
Franziska Greulich   +3 more
doaj   +1 more source

Protocol for fractionation-assisted native ChIP (fanChIP) to capture protein-protein/DNA interactions on chromatin

open access: yesSTAR Protocols, 2021
Summary: Conventional chromatin immunoprecipitation (ChIP) includes many steps that need to be optimized. Here, we have described a protocol of fractionation-assisted native ChIP (fanChIP) which combines subfractionation and native ChIP to purify protein/
Ryo Miyamoto, Akihiko Yokoyama
doaj   +1 more source

An optimized two-step chromatin immunoprecipitation protocol to quantify the associations of two separate proteins and their common target DNA

open access: yesSTAR Protocols, 2021
Summary: Sequential chromatin immunoprecipitation (ChIP) is commonly used to investigate DNA-protein and protein-protein interactions to a specific genomic region. However, it can be tricky to achieve a robust and reproducible signal with sequential ChIP.
Lingli He   +3 more
doaj   +1 more source

Chromatin immunoprecipitation of transcription factors and histone modifications in Comma-Dβ mammary epithelial cells

open access: yesSTAR Protocols, 2021
Summary: Chromatin immunoprecipitation (ChIP) is used to study interactions between proteins and DNA. Nuclear lysates are prepared, and chromatin is fragmented by sonication.
Holly Holliday   +2 more
doaj   +1 more source

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