Results 1 to 10 of about 135,536 (295)
A native chromatin immunoprecipitation (ChIP) protocol for studying histone modifications in strawberry fruits [PDF]
Plant Methods, 2020 Background Covalent modifications of histones and histone variants have great influence on chromatin structure, which is involved in the transcriptional regulation of gene expression.
Xiaorong Huang +4 more
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Chromatin Immunoprecipitation (ChIP) to Assess Histone Marks in Auxin-treated Arabidopsis thaliana Inflorescence Tissue [PDF]
Bio-Protocol, 2020 Chromatin immunoprecipitation coupled with quantitative PCR (ChIP-qPCR) or high-throughput sequencing (ChIP-seq) has become the gold standard for the identification of binding sites of DNA binding proteins and the localization of histone modification on ...
Andre Kuhn, Lars Østergaard
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RNA Chromatin Immunoprecipitation (RNA-ChIP) in Caenorhabditis elegans [PDF]
Bio-Protocol, 2014 The RNA chromatin immunoprecipitation assay (RNA-ChIP) allows detection and quantification of RNA–protein interactions using in vivo cross-linking with formaldehyde followed by immunoprecipitation of the RNA–protein complexes.
Germano Cecere, Alla Grishok
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Chromatin Immunoprecipitation (ChIP) in Mouse T-cell Lines. [PDF]
J Vis Exp, 2017 Signaling pathways regulate gene expression programs via the modulation of the chromatin structure at different levels, such as by post-translational modifications (PTMs) of histone tails, the exchange of canonical histones with histone variants, and nucleosome eviction.
Giaimo BD, Ferrante F, Borggrefe T.
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Protocol: methodology for chromatin immunoprecipitation (ChIP) in
Plant Methods, 2011 We report on a detailed chromatin immunoprecipitation (ChIP) protocol for the unicellular green alga Chlamydomonas reinhardtii. The protocol is suitable for the analysis of nucleosome occupancy, histone modifications and transcription factor binding ...
Strenkert Daniela +2 more
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A Computational Model of Quantitative Chromatin Immunoprecipitation (ChIP) Analysis [PDF]
Cancer Informatics, 2008 Chromatin immunoprecipitation (ChIP) analysis is widely used to identify the locations in genomes occupied by transcription factors (TFs). The approach involves chemical cross-linking of DNA with associated proteins, fragmentation of chromatin by ...
Stephen J. Brandt +4 more
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Chromatin Immunoprecipitation (ChIP) Protocol for Low-abundance Embryonic Samples. [PDF]
J Vis Exp, 2017 Chromatin immunoprecipitation (ChIP) is a widely-used technique for mapping the localization of post-translationally modified histones, histone variants, transcription factors, or chromatin-modifying enzymes at a given locus or on a genome-wide scale. The combination of ChIP assays with next-generation sequencing (i.e., ChIP-Seq) is a powerful approach
Rehimi R +4 more
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Chromatin Immunoprecipitation (ChIP) using Drosophila tissue. [PDF]
J Vis Exp, 2012 Epigenetics remains a rapidly developing field that studies how the chromatin state contributes to differential gene expression in distinct cell types at different developmental stages. Epigenetic regulation contributes to a broad spectrum of biological processes, including cellular differentiation during embryonic development and homeostasis in ...
Tran V, Gan Q, Chen X.
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STAR Protocols, 2021 Summary: Quantifying differential genome occupancy by chromatin immunoprecipitation (ChIP) remains challenging due to variation in chromatin fragmentation, immunoprecipitation efficiencies, and intertube variability. In this protocol, we add heterologous
Franziska Greulich +3 more
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STAR Protocols, 2021 Summary: Conventional chromatin immunoprecipitation (ChIP) includes many steps that need to be optimized. Here, we have described a protocol of fractionation-assisted native ChIP (fanChIP) which combines subfractionation and native ChIP to purify protein/
Ryo Miyamoto, Akihiko Yokoyama
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