Results 11 to 20 of about 135,536 (295)

The Current State of Chromatin Immunoprecipitation (ChIP) from FFPE Tissues. [PDF]

Int J Mol Sci, 2022
Amatori S, Fanelli M.
europepmc   +2 more sources

ChIPprimersDB: a public repository of verified qPCR primers for chromatin immunoprecipitation (ChIP). [PDF]

Nucleic Acids Res, 2019
Kurtenbach S, Reddy R, Harbour JW.
europepmc   +2 more sources

An optimized two-step chromatin immunoprecipitation protocol to quantify the associations of two separate proteins and their common target DNA

STAR Protocols, 2021
Summary: Sequential chromatin immunoprecipitation (ChIP) is commonly used to investigate DNA-protein and protein-protein interactions to a specific genomic region. However, it can be tricky to achieve a robust and reproducible signal with sequential ChIP.
Lingli He, Wentao Yu, Wenxiang Zhang, Lei Zhang   +3 more
doaj   +1 more source

Critical parameters for efficient sonication and improved chromatin immunoprecipitation of high molecular weight proteins [PDF]

, 2016
Solubilization of cross-linked cells followed by chromatin shearing is essential for successful chromatin immunoprecipitation (ChIP). However, this task, typically accomplished by ultrasound treatment, may often become a pitfall of the process, due to ...
Adams, Peter D., Nelson, David M., Pchelintsev, Nikolay A.   +2 more
core   +12 more sources

Chromatin immunoprecipitation of transcription factors and histone modifications in Comma-Dβ mammary epithelial cells

STAR Protocols, 2021
Summary: Chromatin immunoprecipitation (ChIP) is used to study interactions between proteins and DNA. Nuclear lysates are prepared, and chromatin is fragmented by sonication.
Holly Holliday, Amanda Khoury, Alexander Swarbrick   +2 more
doaj   +1 more source

Glycogen Synthase Kinase (GSK) 3β phosphorylates and protects nuclear myosin 1c from proteasome-mediated degradation to activate rDNA transcription in early G1 cells [PDF]

, 2014
Nuclear myosin 1c (NM1) mediates RNA polymerase I (pol I) transcription activation and cell cycle progression by facilitating PCAF-mediated H3K9 acetylation, but the molecular mechanism by which NM1 is regulated remains unclear.
A Cole, A Hay-Koren, A Sarshad, A Vintermist, A-S Tseng, AA Sérandour, AC O'Sullivan, Aishe A. Sarshad, AM Al-Khouri, Anne Von Euler, Bader Al-Muzzaini, CJ Fiol, D Drygin, D Wu, Douglas Lamont, DW Young, E Cavellán, E Kinoshita, E Sanij, F Mohn, GE Zentner, I Grummt, J Grisouard, J Russell, J Ye, JR Woodgett, JR Woodgett, JR Woodgett, L Meijer, Laura Borgonovo-Brandter, LC Fuentealba, M Benavides, M Liu, M Rosner, M Welcker, MA Munoz, Martin Corcoran, N Fomproix, N Visa, Neus Visa, NG Kim, P Cohen, P de Lanerolle, P Percipalle, P Percipalle, Piergiorgio Percipalle, Raffaella Santoro, RS Jope, S Boulon, T Moss, T Tasaki, T Vincent, V Stambolic, VA Smits, VF Taelman, VV Philimonenko, VW Ding, WJ Ryves, YC Tsai   +58 more
core   +14 more sources

A Review of Three Different Studies on Hidden Markov Models for Epigenetic Problems: A Computational Perspective [PDF]

Genomics & Informatics, 2014
Recent technical advances, such as chromatin immunoprecipitation combined with DNA microarrays (ChIp-chip) and chromatin immunoprecipitation-sequencing (ChIP-seq), have generated large quantities of high-throughput data.
Kyung-Eun Lee, Hyun-Seok Park
doaj   +1 more source

Automated ChIPmentation procedure on limited biological material of the human blood fluke Schistosoma mansoni [version 1; peer review: 2 approved, 1 approved with reservations]

Wellcome Open Research, 2022
In living cells, the genetic information stored in the DNA sequence is always associated with chromosomal and extra-chromosomal epigenetic information. Chromatin is formed by the DNA and associated proteins, in particular histones.
Ronaldo de Carvalho Augusto, Christoph Grunau, Chrystelle Lasica, Cristian Chaparro, Anne-Clémence Veillard, Hélène Moné, Agnieszka Zelisko-Schmidt, Gabriel Mouahid   +7 more
doaj   +1 more source

ChIP on Chip: surprising results are often artifacts

BMC Genomics, 2010
Background The method of chromatin immunoprecipitation combined with microarrays (ChIP-Chip) is a powerful tool for genome-wide analysis of protein binding. However, a high background signal is a common phenomenon.
Skarstad Kirsten, Waldminghaus Torsten
doaj   +1 more source

Localized inhibition of protein phosphatase 1 by NUAK1 promotes spliceosome activity and reveals a MYC-sensitive feedback control of transcription. [PDF]

, 2020
Deregulated expression of MYC induces a dependence on the NUAK1 kinase, but the molecular mechanisms underlying this dependence have not been fully clarified.
Ade, C.P., Baluapuri, A., Chang, Y.C., Cossa, G., Eilers, M., Girard, C., Kempa, S., Kumar, A., Kuster, B., Lührmann, R., Mastrobuoni, G., Mumberg, D., Prinz, F., Roeschert, I., Schülein-Völk, C., Silveira Vidal, R., Walz, S., Wolf, E., Wortmann, L.   +18 more
core   +1 more source