Results 11 to 20 of about 595,755 (284)
Protocol: methodology for chromatin immunoprecipitation (ChIP) in
Plant Methods, 2011 We report on a detailed chromatin immunoprecipitation (ChIP) protocol for the unicellular green alga Chlamydomonas reinhardtii. The protocol is suitable for the analysis of nucleosome occupancy, histone modifications and transcription factor binding ...
Strenkert Daniela +2 more
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RNA Chromatin Immunoprecipitation (RNA-ChIP) in Caenorhabditis elegans [PDF]
Bio-Protocol, 2014 The RNA chromatin immunoprecipitation assay (RNA-ChIP) allows detection and quantification of RNA–protein interactions using in vivo cross-linking with formaldehyde followed by immunoprecipitation of the RNA–protein complexes.
Germano Cecere, Alla Grishok
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Chromatin Immunoprecipitation (ChIP) Methodology and Readouts
, 2009 The identification of direct nuclear hormone receptor gene targets provides clues to their contribution to both development and cancer progression. Until recently, the identification of such direct target genes has relied on a combination of expression analysis and in silico searches for consensus binding motifs in gene promoters.
Massie, Charles E, Mills, Ian G
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Chromatin Immunoprecipitation (ChIP) from Mouse Liver Nuclei
, 2020 Understanding the binding of regulatory proteins to their cognate genomic sites is an important step in deciphering transcriptional networks such as the circadian oscillator. Chromatin immunoprecipitation (ChIP) enables the detection and temporal analysis of such binding events in vivo.
Sara S, Fonseca Costa +1 more
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Chromatin Immunoprecipitation (ChIP) Assay in Candida albicans
, 2016 Chromatin immunoprecipitation (ChIP) is a widely used technique which can determine the in vivo association of a specific protein on a particular DNA locus in the genome. In this method cross-linked chromatin is sheared and immunoprecipitated with antibodies raised against a target protein of interest.
Sreyoshi, Mitra +3 more
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Chromatin Immunoprecipitation (ChIP) Analysis of Protein–DNA Interactions
Cold Spring Harbor Protocols, 2018 Here we describe chromatin immunoprecipitation (ChIP), a molecular approach that uses formaldehyde cross-linking to investigate genome structure and function. This approach allows us to determine the distribution of histone modifications (e.g., acetylation, methylation), the deposition of histone variants (H2AZ, H3.3, etc.), and the location of ...
Kim, Tae Hoon, Dekker, Job
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High-Resolution Chromatin Immunoprecipitation: ChIP-Sequencing [PDF]
, 2017 Chromatin immunoprecipitation (ChIP) coupled with next-generation sequencing (NGS) is widely used for studying the nucleoprotein components that are involved in the various cellular processes required for shaping the bacterial nucleoid. This methodology, termed ChIP-sequencing (ChIP-seq), enables the identification of the DNA targets of DNA binding ...
Diaz, Roxanne +3 more
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Protocol: fine-tuning of a Chromatin Immunoprecipitation (ChIP) protocol in tomato [PDF]
Plant Methods, 2010 Background Searching thoroughly for plant cis-elements corresponding to transcription factors is worthwhile to reveal novel gene activation cascades. At the same time, a great deal of research is currently focused on epigenetic events in plants. A widely
Iusem Norberto D +2 more
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Chromatin Preparation from Frozen Tissues for Chromatin Immunoprecipitation (ChIP) Assays
Chromatin immunoprecipitation coupled with next-generation sequencing (ChIP-seq) is a crucial method for examining transcription factor binding and histone modifications across the entire genome. This is a key step in deciphering the complex mechanisms that control cancer immunosurveillance.
Virginia López, Martínez +1 more
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Chromatin Immunoprecipitation (ChIP) in Mouse T-cell Lines. [PDF]
J Vis Exp, 2017 Signaling pathways regulate gene expression programs via the modulation of the chromatin structure at different levels, such as by post-translational modifications (PTMs) of histone tails, the exchange of canonical histones with histone variants, and nucleosome eviction.
Giaimo BD, Ferrante F, Borggrefe T.
europepmc +4 more sources