Results 11 to 20 of about 104,409 (252)

Chromatin Immunoprecipitation (ChIP) Protocol for Low-abundance Embryonic Samples. [PDF]

J Vis Exp, 2017
Chromatin immunoprecipitation (ChIP) is a widely-used technique for mapping the localization of post-translationally modified histones, histone variants, transcription factors, or chromatin-modifying enzymes at a given locus or on a genome-wide scale ...
Rehimi R, Bartusel M, Solinas F, Altmüller J, Rada-Iglesias A.   +4 more
europepmc   +2 more sources

An optimized two-step chromatin immunoprecipitation protocol to quantify the associations of two separate proteins and their common target DNA

STAR Protocols, 2021
Summary: Sequential chromatin immunoprecipitation (ChIP) is commonly used to investigate DNA-protein and protein-protein interactions to a specific genomic region. However, it can be tricky to achieve a robust and reproducible signal with sequential ChIP.
Lingli He, Wentao Yu, Wenxiang Zhang, Lei Zhang   +3 more
doaj   +1 more source

A Review of Three Different Studies on Hidden Markov Models for Epigenetic Problems: A Computational Perspective [PDF]

Genomics & Informatics, 2014
Recent technical advances, such as chromatin immunoprecipitation combined with DNA microarrays (ChIp-chip) and chromatin immunoprecipitation-sequencing (ChIP-seq), have generated large quantities of high-throughput data.
Kyung-Eun Lee, Hyun-Seok Park
doaj   +1 more source

Chromatin immunoprecipitation of transcription factors and histone modifications in Comma-Dβ mammary epithelial cells

STAR Protocols, 2021
Summary: Chromatin immunoprecipitation (ChIP) is used to study interactions between proteins and DNA. Nuclear lysates are prepared, and chromatin is fragmented by sonication.
Holly Holliday, Amanda Khoury, Alexander Swarbrick   +2 more
doaj   +1 more source

Chromatin Immunoprecipitation in the Cnidarian Model System Exaiptasia diaphana

, 2023
Histone post-translational modifications (PTMs) and other epigenetic modifications regulate the chromatin accessibility of genes to the transcriptional machinery, thus affecting an organism's capacity to respond to environmental stimuli.
Della Valle, Francesco, Cui, Guoxin, Orlando, Valerio, Dix, Mascha Fiona, Aranda, Manuel, Liu, Peng   +5 more
core   +1 more source

Automated ChIPmentation procedure on limited biological material of the human blood fluke Schistosoma mansoni [version 1; peer review: 2 approved, 1 approved with reservations]

Wellcome Open Research, 2022
In living cells, the genetic information stored in the DNA sequence is always associated with chromosomal and extra-chromosomal epigenetic information. Chromatin is formed by the DNA and associated proteins, in particular histones.
Ronaldo de Carvalho Augusto, Christoph Grunau, Chrystelle Lasica, Cristian Chaparro, Anne-Clémence Veillard, Hélène Moné, Agnieszka Zelisko-Schmidt, Gabriel Mouahid   +7 more
doaj   +1 more source

Accounting for immunoprecipitation efficiencies in the statistical analysis of ChIP-seq data [PDF]

, 2013
Background: ImmunoPrecipitation (IP) efficiencies may vary largely between different antibodies and between repeated experiments with the same antibody.
Bao, Yanchun,, ’t Hoen, Peter AC, Bao, Yanchun, Veronica Vinciotti, Hoen, P.A.C. 't, Vinciotti, V., Vinciotti, Veronica,, Yanchun Bao, Wit, Ernst,, Bao, Y.C., Ernst Wit, Wit, Ernst, Vinciotti, V, Peter AC ’t Hoen, 't Hoen, Peter A C, Vinciotti, Veronica, Hoen, Peter A.C. ’t,, Bao, Y, 't Hoen, PAC, Wit, E, Wit, E.   +20 more
core   +1 more source

ChIP on Chip: surprising results are often artifacts

BMC Genomics, 2010
Background The method of chromatin immunoprecipitation combined with microarrays (ChIP-Chip) is a powerful tool for genome-wide analysis of protein binding. However, a high background signal is a common phenomenon.
Skarstad Kirsten, Waldminghaus Torsten
doaj   +1 more source

Profiling chromatin regulatory landscape: insights into the development of ChIP-seq and ATAC-seq

Molecular Biomedicine, 2020
Chromatin regulatory landscape plays a critical role in many disease processes and embryo development. Epigenome sequencing technologies such as chromatin immunoprecipitation sequencing (ChIP-seq) and assay for transposase-accessible chromatin with high ...
Shaoqian Ma, Yongyou Zhang
doaj   +1 more source

Protocol to apply spike-in ChIP-seq to capture massive histone acetylation in human cells

STAR Protocols, 2021
Summary: Inhibition of histone deacetylases causes rapid and robust acetylation of histones. In this case, histone acetylation is likely increased on nearly every nucleosome, and the per-cell DNA/chromatin yield in chromatin immunoprecipitation (ChIP ...
Di Wu, Liguo Wang, Haojie Huang
doaj   +1 more source