Results 41 to 50 of about 135,536 (295)

Chromatin Preparation and Chromatin Immuno-precipitation from Drosophila Embryos

, 2016
This protocol provides specific details on how to perform Chromatin immunoprecipitation (ChIP) from Drosophila embryos. ChIP allows the matching of proteins or histone modifications to specific genomic regions.
Iovino, N., Latrielle, D., Löser, E.
core   +1 more source

Chromatin status and transcription factor binding to gonadotropin promoters in gonadotrope cell lines. [PDF]

, 2017
BackgroundProper expression of key reproductive hormones from gonadotrope cells of the pituitary is required for pubertal onset and reproduction. To further our understanding of the molecular events taking place during embryonic development, leading to ...
Brayman, Melissa J, Hoffmann, Hanne M, Iyer, Anita K, Mellon, Pamela L, Ngo, Cindy, Sunshine, Mary Jean, Xie, Huimin   +6 more
core   +3 more sources

Targeted Derepression of the Human Immunodeficiency Virus Type 1 Long Terminal Repeat by Pyrrole-Imidazole Polyamides [PDF]

, 2002
The host factor LSF represses the human immunodeficiency virus type 1 long terminal repeat (LTR) by mediating recruitment of histone deacetylase. We show that pyrrole-imidazole polyamides targeted to the LTR can specifically block LSF binding both in ...
Coull, Jason J., Dervan, Peter B., He, Guocheng, Margolis, David M., Melander, Christian, Rucker, Victor C.   +5 more
core   +1 more source

HTRIdb: an open-access database for experimentally verified human transcriptional regulation interactions [PDF]

, 2012
Background: The modeling of interactions among transcription factors (TFs) and their respective target genes (TGs) into transcriptional regulatory networks is important for the complete understanding of regulation of biological processes.
Luiz A. Bovolenta, Marcio L. Acencio, Ney Lemke   +2 more
core   +4 more sources

Epigenomic profiling of archived FFPE tissues by enhanced PAT-ChIP (EPAT-ChIP) technology

Clinical Epigenetics, 2018
Background The introduction of pathology tissue-chromatin immunoprecipitation (PAT-ChIP), a technique allowing chromatin immunoprecipitation (ChIP) from formalin-fixed paraffin-embedded (FFPE) tissues, has extended the application of chromatin studies to
Stefano Amatori, Giuseppe Persico, Claudio Paolicelli, Roman Hillje, Nora Sahnane, Francesco Corini, Daniela Furlan, Lucilla Luzi, Saverio Minucci, Marco Giorgio, Pier Giuseppe Pelicci, Mirco Fanelli   +11 more
doaj   +1 more source

LSD1-mediated enhancer silencing attenuates retinoic acid signalling during pancreatic endocrine cell development. [PDF]

, 2020
Developmental progression depends on temporally defined changes in gene expression mediated by transient exposure of lineage intermediates to signals in the progenitor niche.
Benner, Christopher W, Geusz, Ryan J, Harrington, Austin R, Jhala, Ulupi S, Matta, Ileana, Patel, Nisha A, Rosenfeld, Michael G, Sander, Maike, Shih, Hung-Ping, Vinckier, Nicholas K, Wang, Allen, Wang, Jianxun, Wang, Jinzhao, Wetton, Nichole, Wortham, Matthew   +14 more
core   +2 more sources

Protocol for chromatin immunoprecipitation of histone modifications in frozen adipose tissue

STAR Protocols
Summary: Chromatin immunoprecipitation (ChIP) combined with sequencing has revolutionized our understanding of gene regulation; however, its application to frozen adipose tissue presents unique challenges due to the high levels of lipid content. Here, we
Akin Cayir, Tone M. Tannæs, Sadia Saeed, Matthias Blüher, Yvonne Böttcher   +4 more
doaj   +1 more source

Dual agarose magnetic (DAM) ChIP

BMC Research Notes, 2009
Background Chromatin immunoprecipitation (ChIP) has become a very popular technique to study epigenetic regulation because it can be used to identify proteins and protein modifications present at specific locations in chromatin.
Balakrishnan Lata, Milavetz Barry
doaj   +1 more source

Distinct distribution of ectopically expressed histone variants H2A.Bbd and MacroH2A in open and closed chromatin domains. [PDF]

PLoS ONE, 2012
BACKGROUND:It becomes increasingly evident that nuclesomes are far from being identical to each other. This nucleosome diversity is due partially to the existence of histone variants encoded by separate genes.
Elena S Ioudinkova, Ana Barat, Andrey Pichugin, Elena Markova, Ilya Sklyar, Iryna Pirozhkova, Chloe Robin, Marc Lipinski, Vasily Ogryzko, Yegor S Vassetzky, Sergey V Razin   +10 more
doaj   +1 more source

Comparative analyses of CTCF and BORIS occupancies uncover two distinct classes of CTCF binding genomic regions. [PDF]

, 2015
BackgroundCTCF and BORIS (CTCFL), two paralogous mammalian proteins sharing nearly identical DNA binding domains, are thought to function in a mutually exclusive manner in DNA binding and transcriptional regulation.ResultsHere we show that these two ...
Boukaba, Abdelhalim, Espinoza, Celso A, Kang, Sungyun, Kosaka-Suzuki, Natsuki, Lobanenkov, Victor V, Loukinov, Dmitri, Méndez-Catalá, Claudia Fabiola, Nagarajan, Vijayaraj, Pugacheva, Elena M, Rasko, John EJ, Ren, Bing, Rivero-Hinojosa, Samuel, Robinson, Susan, Strunnikov, Alexander V, Suzuki, Teruhiko, Ye, Zhen   +15 more
core   +3 more sources