Results 41 to 50 of about 595,755 (284)

Protocol to capture transcription factor-mediated 3D chromatin interactions using affinity tag-based BL-HiChIP

STAR Protocols, 2023
Summary: Pioneer transcription factors (TFs) can directly establish higher-order chromatin interactions to instruct gene transcription. Here, we present a protocol for capturing TF-mediated 3D chromatin interactions using affinity tag-based bridge linker
Ruimin Ren, Yao Hua, Heng Wang
doaj   +1 more source

Chromatin state analysis of the barley epigenome reveals a higher-order structure defined by H3K27me1 and H3K27me3 abundance [PDF]

, 2015
This work was supported by grant BBSRC BB/I1022899/1 ‘The diversity and evolution of the gene component of the barley pericentromeric heterochromatin’.Combinations of histones carrying different covalent modifications are a major component of epigenetic ...
Dhillon, Taniya, Milne, Iain, Katie Baker, Baker, Katie, Colas, Isabelle, Taniya Dhillon, Micha Bayer, Bayer, Micha, Milne, Linda, Linda Milne, Flavell, Andrew J., Iain Milne, Andrew J. Flavell, Cook, Nicola, Nicola Cook, Isabelle Colas   +15 more
core   +1 more source

Impact of chromatin structures on DNA processing for genomic analyses. [PDF]

PLoS ONE, 2009
Chromatin has an impact on recombination, repair, replication, and evolution of DNA. Here we report that chromatin structure also affects laboratory DNA manipulation in ways that distort the results of chromatin immunoprecipitation (ChIP) experiments. We
Leonid Teytelman, Bilge Ozaydin, Oliver Zill, Philippe Lefrançois, Michael Snyder, Jasper Rine, Michael B Eisen   +6 more
doaj   +1 more source

An optimized protocol for chromatin immunoprecipitation from murine inguinal white adipose tissue

STAR Protocols, 2023
Summary: Chromatin immunoprecipitation (ChIP) protocols have been used to reveal protein-DNA interactions of various cell types and tissues; however, optimization is required for each specific type of sample.
Antonia Katsouda, Dimitrios Valakos, Giannis Vatsellas, Dimitris Thanos, Andreas Papapetropoulos   +4 more
doaj   +1 more source

Development of an efficient chromatin immunoprecipitation method to investigate protein-DNA interaction in oleaginous castor bean seeds. [PDF]

PLoS ONE, 2018
Chromatin immunoprecipitation (ChIP) is usually a reliable technique to find the binding sites of a transcription factor. In the current study, we developed a suitable ChIP method using developing castor bean seeds.
Mohammad Enamul Haque, Bing Han, Bin Wang, Yue Wang, Aizhong Liu   +4 more
doaj   +1 more source

Chromatin immunoprecipitation with mouse adipocytes using hypotonic buffer to enrich nuclear fraction before fixation

STAR Protocols, 2023
Summary: Chromatin immunoprecipitation (ChIP) experiments with differentiated adipocytes are challenging because lipid droplets interfere with immunoprecipitation efficiency. Here, the author describes optimized procedures to minimize the burden of lipid
Yuta Hiraike
doaj   +1 more source

Proteomic interrogation of human chromatin. [PDF]

, 2011
Chromatin proteins provide a scaffold for DNA packaging and a basis for epigenetic regulation and genomic maintenance. Despite understanding its functional roles, mapping the chromatin proteome (i.e. the "Chromatome") is still a continuing process. Here,
LeRoy, Gary, Christodoulos A Floudas, Floudas, Christodoulos A., Barry M Zee, Garcia, Benjamin A., Richard C Baliban, Baliban, Richard C., Sandra B Hake, Torrente, Mariana P., Zee, Barry M., Hake, Sandra B., Christodoulos A. Floudas, Barry M. Zee, Nicolas L. Young, Mariana P. Torrente, Gary LeRoy, Richard C. Baliban, Sandra B. Hake, Benjamin A. Garcia, Nicolas L Young, Benjamin A Garcia, Young, Nicolas L., Mariana P Torrente   +22 more
core   +2 more sources

Epigenomic profiling of archived FFPE tissues by enhanced PAT-ChIP (EPAT-ChIP) technology

Clinical Epigenetics, 2018
Background The introduction of pathology tissue-chromatin immunoprecipitation (PAT-ChIP), a technique allowing chromatin immunoprecipitation (ChIP) from formalin-fixed paraffin-embedded (FFPE) tissues, has extended the application of chromatin studies to
Stefano Amatori, Giuseppe Persico, Claudio Paolicelli, Roman Hillje, Nora Sahnane, Francesco Corini, Daniela Furlan, Lucilla Luzi, Saverio Minucci, Marco Giorgio, Pier Giuseppe Pelicci, Mirco Fanelli   +11 more
doaj   +1 more source

Protocol for chromatin immunoprecipitation of histone modifications in frozen adipose tissue

STAR Protocols
Summary: Chromatin immunoprecipitation (ChIP) combined with sequencing has revolutionized our understanding of gene regulation; however, its application to frozen adipose tissue presents unique challenges due to the high levels of lipid content. Here, we
Akin Cayir, Tone M. Tannæs, Sadia Saeed, Matthias Blüher, Yvonne Böttcher   +4 more
doaj   +1 more source

Dual agarose magnetic (DAM) ChIP

BMC Research Notes, 2009
Background Chromatin immunoprecipitation (ChIP) has become a very popular technique to study epigenetic regulation because it can be used to identify proteins and protein modifications present at specific locations in chromatin.
Balakrishnan Lata, Milavetz Barry
doaj   +1 more source