The laminA/NF-Y protein complex reveals an unknown transcriptional mechanism on cell proliferation [PDF]
, 2016 Lamin A is a component of the nuclear matrix that also controls proliferation by largely unknown mechanisms. NF-Y is a ubiquitous protein involved in cell proliferation composed of three subunits (-YA -YB -YC) all required for the DNA ...
Alonzi, Tonino +16 more
core +2 more sources
An optimized protocol for chromatin immunoprecipitation from murine inguinal white adipose tissue
STAR Protocols, 2023 Summary: Chromatin immunoprecipitation (ChIP) protocols have been used to reveal protein-DNA interactions of various cell types and tissues; however, optimization is required for each specific type of sample.
Antonia Katsouda +4 more
doaj +1 more source
The enzymes LSD1 and Set1A cooperate with the viral protein HBx to establish an active hepatitis B viral chromatin state [PDF]
, 2016 Indexación: Web of ScienceWith about 350 million people chronically infected around the world hepatitis B is a major health problem. Template for progeny HBV synthesis is the viral genome, organized as a minichromosome (cccDNA) inside the hepatocyte ...
Alarcon, V +9 more
core +2 more sources
Chromatin Immunoprecipitation (ChIP), Streptavidin and ATP-agarose Mediated Pull-down Analyses
Bio-Protocol, 2013 Epstein-Barr virus (EBV) nuclear antigen 2 (EBNA2) induces expression of both viral and cellular genes in virus infected B cells by mimicking activated Notch receptors (Notch-IC) that mediate transcription activation through binding to the repressing ...
Cheng-Der Liu +8 more
doaj +1 more source
STAR Protocols, 2023 Summary: Pioneer transcription factors (TFs) can directly establish higher-order chromatin interactions to instruct gene transcription. Here, we present a protocol for capturing TF-mediated 3D chromatin interactions using affinity tag-based bridge linker
Ruimin Ren, Yao Hua, Heng Wang
doaj +1 more source
DNA-encoded nucleosome occupancy is associated with transcription levels in the human malaria parasite Plasmodium falciparum. [PDF]
, 2014 BackgroundIn eukaryotic organisms, packaging of DNA into nucleosomes controls gene expression by regulating access of the promoter to transcription factors.
Bunnik, Evelien M +6 more
core +3 more sources
Impact of chromatin structures on DNA processing for genomic analyses. [PDF]
PLoS ONE, 2009 Chromatin has an impact on recombination, repair, replication, and evolution of DNA. Here we report that chromatin structure also affects laboratory DNA manipulation in ways that distort the results of chromatin immunoprecipitation (ChIP) experiments. We
Leonid Teytelman +6 more
doaj +1 more source
TBL1 is required for the mesenchymal phenotype of transformed breast cancer cells [PDF]
, 2019 The epithelial-to-mesenchymal transition (EMT) and its reversion (MET) are related to tumor cell dissemination and migration, tumor circulating cell generation, cancer stem cells, chemoresistance, and metastasis formation.
García Martínez, Jorge +4 more
core +2 more sources
Development of an efficient chromatin immunoprecipitation method to investigate protein-DNA interaction in oleaginous castor bean seeds. [PDF]
PLoS ONE, 2018 Chromatin immunoprecipitation (ChIP) is usually a reliable technique to find the binding sites of a transcription factor. In the current study, we developed a suitable ChIP method using developing castor bean seeds.
Mohammad Enamul Haque +4 more
doaj +1 more source
STAR Protocols, 2023 Summary: Chromatin immunoprecipitation (ChIP) experiments with differentiated adipocytes are challenging because lipid droplets interfere with immunoprecipitation efficiency. Here, the author describes optimized procedures to minimize the burden of lipid
Yuta Hiraike
doaj +1 more source