Human THO–Sin3A interaction reveals new mechanisms to prevent R-loops that cause genome instability [PDF]
, 2017 R-loops, formed by co-transcriptional DNA–RNA hybrids and a displaced DNA single strand (ssDNA), fulfill certain positive regulatory roles but are also a source of genomic instability.
Aguilera López, Andrés +5 more
core +1 more source
FEBS Open Bio, EarlyView.The MRP4 transporter exports several drugs and signaling molecules. Here, we identified key promoter elements regulating basal MRP4 expression. Using reporter assays, we defined a conserved region with essential Sp1 and contributory Ets sites, which controlled basal MRP4 expression.
Debora Singer +7 more
wiley +1 more source
The Histone Deacetylase SIRT6 Restrains Transcription Elongation via Promoter-Proximal Pausing. [PDF]
, 2019 Transcriptional regulation in eukaryotes occurs at promoter-proximal regions wherein transcriptionally engaged RNA polymerase II (Pol II) pauses before proceeding toward productive elongation.
Ablondi, Eileen +20 more
core +1 more source
Methods for Scarless, Selection-Free Generation of Human Cells and Allele-Specific Functional Analysis of Disease-Associated SNPs and Variants of Uncertain Significance. [PDF]
, 2017 With the continued emergence of risk loci from Genome-Wide Association studies and variants of uncertain significance identified from patient sequencing, better methods are required to translate these human genetic findings into improvements in public ...
Carvajal-Carmona, Luis G +4 more
core +4 more sources
Advanced Functional Materials, EarlyView.Butterfly wing scales are intricate cuticular functional nanosctructures. This perspective suggests that spatially varying material properties, cytoskeletal constraints, and growth‐driven mechanical instabilities shape the resulting nanoscale architectures created from single cells.
Anupama Prakash +10 more
wiley +1 more source
Protocol: fine-tuning of a Chromatin Immunoprecipitation (ChIP) protocol in tomato
Plant Methods, 2010 Background Searching thoroughly for plant cis-elements corresponding to transcription factors is worthwhile to reveal novel gene activation cascades. At the same time, a great deal of research is currently focused on epigenetic events in plants. A widely
Iusem Norberto D +2 more
doaj +1 more source
Slx5/Slx8-dependent ubiquitin hotspots on chromatin contribute to stress tolerance [PDF]
, 2019 Chromatin is a highly regulated environment, and protein association with chromatin is often controlled by post-translational modifications and the corresponding enzymatic machinery.
Habermann, B. +6 more
core +3 more sources
3D Soft Hydrogels Induce Human Mesenchymal Stem Cells “Deep” Quiescence
Advanced Healthcare Materials, EarlyView.Three‐dimensional soft hydrogels mimicking the bone marrow niche induce deep quiescence in human mesenchymal stem cells. Unlike 2D culture, 3D matrices halt proliferation, regulate cell‐cycle and quiescence markers, and downregulate mTORC1 signaling, preserving stem cell phenotype and therapeutic potential ex vivo.
David Boaventura Gomes +11 more
wiley +1 more source
An Alternative Approach to ChIP-Seq Normalization Enables Detection of Genome-Wide Changes in Histone H3 Lysine 27 Trimethylation upon EZH2 Inhibition. [PDF]
PLoS ONE, 2016 Chromatin immunoprecipitation and DNA sequencing (ChIP-seq) has been instrumental in inferring the roles of histone post-translational modifications in the regulation of transcription, chromatin compaction and other cellular processes that require ...
Brian Egan +16 more
doaj +1 more source
STAR Protocols, 2023 Summary: Chromatin immunoprecipitation (ChIP) assay is widely used for investigating the interaction between DNA and DNA-binding proteins such as transcription factors, co-factors, or chromatin-associated proteins.
Wentong Fang, Chengheng Liao, Qing Zhang
doaj +1 more source