Results 71 to 80 of about 104,409 (252)

ZDHHC9‐Mediated Palmitoylation of ACSL4 Drives Ferroptosis in Diabetes Mellitus–Induced Erectile Dysfunction

Advanced Science, EarlyView.
ABSTRACT Diabetes mellitus‐induced erectile dysfunction (DMED) is a highly prevalent complication among diabetic patients; however, its underlying pathogenic mechanisms remain incompletely understood. Metabolic disorder is a hallmark of diabetes, yet its precise contribution to DMED progression is not well defined.
Wanyang Guo, Ming Xiao, Mengjun Huang, Dongzi Peng, Ruijiang Zeng, Ruilin Liu, Yuanqiao Zhao, Zhihan Ouyang, Yulong Hong, Zexian Ding, Zhuo Xing, Hao Su, Jinxiang Wang, Wenjun Mao, Xin Jin   +14 more
wiley   +1 more source

Chromatin immunoprecipitation (ChIP) methodology and readouts

, 2009
The identification of direct nuclear hormone receptor gene targets provides clues to their contribution to both development and cancer progression. Until recently, the identification of such direct target genes has relied on a combination of expression ...
Massie, Charles E, Charles E. Massie, Mills, Ian G; id_orcid, Ian G. Mills   +3 more
core   +1 more source

CD4+ Tregs Drive Post‐Ischemic Sprouting Angiogenesis via Endothelial YY1/MAML1 Reactivation

Advanced Science, EarlyView.
ABSTRACT Microvascular complications of diabetes are chronic diseases of small vessels. We previously found that CD4+ regulatory T‐cells (Tregs) are markedly reduced in type 2 diabetes (T2D) after ischemic injury in both mice and humans, and that Treg deficiency in immunodeficient mice impairs vascular regeneration.
Hang Qu, Cheng Kiu Ho, Jitao Liu, Lei Cui, Yangfeng Hou, Cai Liang, Wenchu Ye, Randolph H. L. Wong, Shohei Hori, Bin Zhou, Kathy O. Lui   +10 more
wiley   +1 more source

Plant Chromatin Immunoprecipitation

, 2010
Development of multicellular organisms is based on specialized gene expression programs. Because chromatin establishes the environment for transcription, understanding composition and dynamics of chromatin is an important part of developmental biology ...
Claudia Köhler, Villar, C., Köhler, C., Corina B.R. Villar   +3 more
core   +1 more source

Pax6 interactions with chromatin and identification of its novel direct target genes in lens and forebrain. [PDF]

, 2013
Pax6 encodes a specific DNA-binding transcription factor that regulates the development of multiple organs, including the eye, brain and pancreas. Previous studies have shown that Pax6 regulates the entire process of ocular lens development.
Tessa Walcher, Jovica Ninkovic (104237), David C Beebe, Deyou Zheng (7943), Ying Yang (24175), Xie, Q., Jie Huang, Louise Wolf (104240), Ariel Vitenzon (104242), Vitenzon, Ariel, Magdalena Götz, Zavadil, J., Wolf, L., Deyou Zheng, Götz, M., Yang, Ying, Ales Cvekl, Wolf, Louise, Tessa Walcher (104238), Yang, Y., Cvekl, A., Zheng, D.Y., Louise Wolf, Ninkovic, J., Jovica Ninkovic, Ariel Vitenzon, Götz, Magdalena, Jiri Zavadil (10270), Walcher, Tessa, Magdalena Götz (32199), Zavadil, Jiri, Qing Xie, Ninkovic, Jovica, Walcher, T., David C. Beebe (104247), Beebe, D.C., Zheng, Deyou, Huang, Jie, Cvekl, Ales, David C. Beebe, Xie, Qing, Jie Huang (104235), Ales Cvekl (104250), Vitenzon, A., Beebe, David C., Qing Xie (104232), Ying Yang, Jiri Zavadil, Huang, J.   +48 more
core   +1 more source

Inhibition of SIRT7 Overcomes Radioresistance in Pancreatic Neuroendocrine Tumors by Reactivating MEN1 Expression

Advanced Science, EarlyView.
Pancreatic neuroendocrine tumors frequently silence MEN1 through epigenetic mechanisms. Here, SIRT7 recruits DNMT1 to the MEN1 promoter, drives hypermethylation, and enhances DNA repair. Inhibiting SIRT7 restores MEN1, reduces MRN complex abundance, impairs double‐strand break repair, and sensitizes PanNET models to radiation, supporting SIRT7 as a ...
Jianyun Jiang, Yan Wang, Yi Qin, Luohai Chen, Xiaowu Xu, Guixiong Fan, Desheng Jing, Miaoyan Wei, Xianjun Yu, Junfeng Xu, Shunrong Ji, Jie Chen   +11 more
wiley   +1 more source

Molecular Glue cc‐885 Inhibits VHL‐Deficient Clear Cell Renal Cell Carcinoma via ETS1 Degradation

Advanced Science, EarlyView.
VHL‐deficient kidney cancer lacks effective treatments. This study reveals that the molecular glue degrader cc‐885 hijacks the cellular recycling system to selectively destroy the oncogenic protein ETS1, effectively killing VHL‐mutant tumors. Combining CC‐885 with the approved drug belzutifan achieves powerful synergy, offering a promising new ...
Taowei Yang, Qihao Li, Kun Ye, Kezhi Liu, Mi Zhou, Minyu Chen, Lican Liao, Kangbo Huang, Zhu Wang, Qiong Deng, Jieyan Wang, Meiyu Jin, Xu Chen, Hui Liang, Jiaxing Zhang, Junhang Luo   +15 more
wiley   +1 more source

Automated ChIPmentation procedure on limited biological material of the human blood fluke Schistosoma mansoni [version 2; peer review: 2 approved, 1 approved with reservations]

Wellcome Open Research
In living cells, the genetic information stored in the DNA sequence is always associated with chromosomal and extra-chromosomal epigenetic information. Chromatin is formed by the DNA and associated proteins, in particular histones.
Ronaldo de Carvalho Augusto, Christoph Grunau, Chrystelle Lasica, Cristian Chaparro, Anne-Clémence Veillard, Hélène Moné, Agnieszka Zelisko-Schmidt, Gabriel Mouahid   +7 more
doaj   +1 more source

m6A‐Mediated Glycolysis by IL‐37 Drives T Cell Metabolic Reprogramming to Regulate Colitis

Advanced Science, EarlyView.
This study identifies an IL‐37/SIGIRR‐METTL14 regulatory axis that suppresses global m6A modification in CD4+ T cells. IL‐37 signaling, mediated through SIGIRR, inhibits IRAK4 and JNK phosphorylation, leading to downregulation of the methyltransferase METTL14.
Xiaoyan Wang, Jiadong Yu, Guolin Li, Ya Li, Fanlian Zeng, Jing Hu, Yifan Zhou, Chengcheng Yue, Qixiang Zhao, Chen Zhang, Hong Zhou, Fulei Zhao, Yawen Hu, Pei Zhou, Linna Gu, Yuting Feng, Mingxiang He, Shishi Huang, Xiaochi Sun, Xinmeng Wang, Jie Tang, Xinyu Zeng, Wenling Wu, Nongyu Huang, Xinai Cui, Kaijun Cui, Jiong Li   +26 more
wiley   +1 more source

An efficient targeted nuclease strategy for high-resolution mapping of DNA binding sites

eLife, 2017
We describe Cleavage Under Targets and Release Using Nuclease (CUT&RUN), a chromatin profiling strategy in which antibody-targeted controlled cleavage by micrococcal nuclease releases specific protein-DNA complexes into the supernatant for paired-end DNA
Peter J Skene, Steven Henikoff
doaj   +1 more source