Results 91 to 100 of about 135,536 (295)

DOT1L Drives Endothelial‐to‐Mesenchymal Transition and Fibrotic Vascular Remodeling via H3K79 Methylation

Advanced Science, EarlyView.
DOT1L as a central epigenetic regulator of EndoMT and pulmonary fibrosis. Acting as an early epigenetic switch, it translates TGFβ–SMAD signaling into H3K79me2‐mediated chromatin remodeling, selectively activates fibrosis‐related genes, and primes ECs for rapid mesenchymal transition.
Yaofeng Wang, Xing Peng, Jingjing Chen, Yun Zhang, Tinghong Zhang, Jingyuan Zhang, Jiaying Fan, Hui Zheng, Qiaoyuan Liu, Zhimin Song, Zhan‐Peng Huang, Shu Meng   +11 more
wiley   +1 more source

Automated ChIPmentation procedure on limited biological material of the human blood fluke Schistosoma mansoni [version 2; peer review: 2 approved, 1 approved with reservations]

Wellcome Open Research
In living cells, the genetic information stored in the DNA sequence is always associated with chromosomal and extra-chromosomal epigenetic information. Chromatin is formed by the DNA and associated proteins, in particular histones.
Ronaldo de Carvalho Augusto, Christoph Grunau, Chrystelle Lasica, Cristian Chaparro, Anne-Clémence Veillard, Hélène Moné, Agnieszka Zelisko-Schmidt, Gabriel Mouahid   +7 more
doaj   +1 more source

YAP integrates the regulatory Snail/HNF4α circuitry controlling epithelial/hepatocyte differentiation [PDF]

, 2019
Yes-associated protein (YAP) is a transcriptional co-factor involved in many cell processes, including development, proliferation, stemness, differentiation, and tumorigenesis.
Amicone, Laura, Battistelli, Cecilia, Cicchini, Carla, Consalvi, Veronica, Cozzolino, Angela Maria, Marchetti, Alessandra, Noce, Valeria, Strippoli, Raffaele, Tripodi, Marco   +8 more
core   +1 more source

ZDHHC9‐Mediated Palmitoylation of ACSL4 Drives Ferroptosis in Diabetes Mellitus–Induced Erectile Dysfunction

Advanced Science, EarlyView.
ABSTRACT Diabetes mellitus‐induced erectile dysfunction (DMED) is a highly prevalent complication among diabetic patients; however, its underlying pathogenic mechanisms remain incompletely understood. Metabolic disorder is a hallmark of diabetes, yet its precise contribution to DMED progression is not well defined.
Wanyang Guo, Ming Xiao, Mengjun Huang, Dongzi Peng, Ruijiang Zeng, Ruilin Liu, Yuanqiao Zhao, Zhihan Ouyang, Yulong Hong, Zexian Ding, Zhuo Xing, Hao Su, Jinxiang Wang, Wenjun Mao, Xin Jin   +14 more
wiley   +1 more source

An efficient targeted nuclease strategy for high-resolution mapping of DNA binding sites

eLife, 2017
We describe Cleavage Under Targets and Release Using Nuclease (CUT&RUN), a chromatin profiling strategy in which antibody-targeted controlled cleavage by micrococcal nuclease releases specific protein-DNA complexes into the supernatant for paired-end DNA
Peter J Skene, Steven Henikoff
doaj   +1 more source

Identification of functional cis-regulatory elements by sequential enrichment from a randomized synthetic DNA library [PDF]

, 2013
BACKGROUND: The identification of endogenous cis-regulatory DNA elements (CREs) responsive to endogenous and environmental cues is important for studying gene regulation and for biotechnological applications but is labor and time intensive. Alternatively,
Ahmadinejad, N., Colby, T., Roccaro, M., Somssich, I.   +3 more
core   +2 more sources

Mapping Genetic Regulation of Transcription to Identify Functional Variants and Genes Associated with Pancreatic Cancer Risk

Advanced Science, EarlyView.
Integration of a pancreatic eQTL map with a GWAS meta‐analysis identifies 82 putative functional variants and 15 genes. The association between rs11102484 and pancreatic cancer risk is observed in a total of 5699 cases and 8467 controls. The G allele of rs11102484 weakens ZNF263 binding and the silencer‐promoter interaction, thereby increasing ST7L ...
Xiaoyang Wang, Hui Geng, Zhengyan Yao, Yuan Jiang, Can Chen, Zequn Lu, Shuangshuang Tian, Ming Zhang, Ruiyan Liu, Chenxi Feng, Bin Li, Xiaoping Miao, Jianbo Tian, Shaokai Zhang, Ying Zhu   +14 more
wiley   +1 more source

ArgR is an essential local transcriptional regulator of the arcABC-operon in Streptococcus suis and crucial for biological fitness in acidic environment [PDF]

, 2011
Streptococcus suis is one of the most important pathogens in pigs and can also cause severe infections in humans. Despite its clinical relevance very little is known about the factors contributing to its virulence.
Benga, L., Fulde, M., Goethe, R., Greeff, A., de, Smith, H.E., Valentin-Weigand, P., Willenborg, J.   +6 more
core   +2 more sources

Chromatin Immunoprecipitation (Chip) Assay

, 2015
Chromatin immunoprecipitation (ChIP) assay is a powerful tool to study protein-DNA interaction and chromatin changes associated with gene expression. In the related article, we used ChIP assay to evaluate CCA1 binding to the CCA1, LHY, TOC1 and GI upstream region, as well as histone modifications at these loci in A. thaliana, A.
openaire   +1 more source

Hypofractionated Radiotherapy Induces ISG15+MHC‐I+ Neutrophils to Augment Anti‐Tumor Immunity and Prime Immune Checkpoint Blockade Responses in Rectal Cancer

Advanced Science, EarlyView.
ABSTRACT Our previous clinical trials had demonstrated that neoadjuvant hypofractionated radiotherapy (HFRT) combined with immunotherapy yields promising clinical outcomes in locally advanced rectal cancer (LARC). However, this combined modality benefits only a subset of patients, highlighting the need to uncover the mechanisms underlying how ...
Lichao Liu, Haihong Wang, Mingjie Li, Qian Xu, Linlin Zheng, Chaoqun Han, Zhenwei Zou, Jinghua Ren, Xiaorong Dong, Peng Zhang, Kaixiong Tao, Zhenyu Lin, Tao Zhang   +12 more
wiley   +1 more source