Results 91 to 100 of about 595,755 (284)
Chromatin Preparation and Chromatin Immunoprecipitation from Drosophila Heads
, 2023 Chromatin immunoprecipitation (ChIP) is a widely used method to map protein-DNA interactions in vivo. Formaldehyde cross-linked chromatin is fragmented, and the protein of interest is immunoprecipitated using a specific antibody.
Cernilogar FM, Andrenacci D
core +1 more source
Deltacoronavirus Modulates circRNA cGLIS3 Metabolism to Evade Host Antiviral Response
Advanced Science, EarlyView.This study reveals that both deltacoronavirus nucleocapsid protein and host RNA binding protein IGF2BP2 promote circular RNA GLIS3 (cGLIS3) biogenesis by binding to GLIS3 pre‐mRNA. The m6A modification‐mediated cGLIS3‐IGF2BP2 interaction weakens RNase L‐mediated degradation of cGLIS3 while facilitates a ubiquitin‐dependent degradation of IGF2BP2, thus ...
Liuyang Du +10 more
wiley +1 more source
Advanced Science, EarlyView.A schematic model summarizing GNA15's mechanism in mediating macrophage M2 polarization and Gemcitabine resistance. ABSTRACT The complexity of pancreatic ductal adenocarcinoma (PDAC) progression, coupled with the lack of effective immunotherapy, underscores the imperative to deepen our understanding of its mechanisms and identify suitable immune ...
Weihui Guo +8 more
wiley +1 more source
Chromatin Immunoprecipitation (ChIP) of Heat Shock Protein 90 (Hsp90). [PDF]
Chromatin immunoprecipitation followed by sequencing (ChIP-seq) is a widely used technique for genome-wide mapping of protein-DNA interactions and epigenetic marks in vivo.
core +2 more sources
An efficient chromatin immunoprecipitation (ChIP) protocol for studying histone modifications in peach reproductive tissues. [PDF]
Plant Methods, 2022 Canton M +5 more
europepmc +1 more source
Advanced Science, EarlyView.Hypoxia triggers a dual mechanism for BHLHE40 upregulation: reactive oxygen species (ROS)‐dependent post‐translational modification and hypoxia‐inducible factor (HIF)‐dependent transcriptional activation. Under hypoxic conditions, mitochondrial BHLHE40 accumulates and senses ROS via cysteine thiol oxidation and disulfide‐linked homodimer formation ...
Jia Liu +13 more
wiley +1 more source
, 2010 Chromatin immunoprecipitation (ChIP) is a powerful technique to study interactions between transcription factors (TFs) and DNA in vivo. For genome-wide de novo discovery of TF-binding sites, the DNA that is obtained in ChIP experiments needs to be ...
Angenent, G.C. +5 more
core +1 more source
Proteotoxic stress reprograms the chromatin landscape of SUMO modification [PDF]
, 2015 The small ubiquitin-like modifier 2 (SUMO-2) is required for survival when cells are exposed to treatments that induce proteotoxic stress by causing the accumulation of misfolded proteins.
Seifert, Anne +3 more
core +1 more source
High-intensity UV laser ChIP-seq for the study of protein-DNA interactions in living cells
Nature Communications, 2017 Chromatin immunoprecipitation followed by DNA sequencing (ChIP-seq) can map transcription factor binding to a given genome. Here, Steube and colleagues devise a ChIP-seq technique based on photochemical crosslinking by high-intensity ultraviolet laser ...
Arndt Steube +3 more
doaj +1 more source
UFL1‐Mediated UFMylation of ENO1 Restrains Aerobic Glycolysis and Colorectal Cancer Progression
Advanced Science, EarlyView.UFMylation restrains aerobic glycolysis and colorectal cancer progression by modifying the glycolytic enzyme ENO1. Pharmacological enhancement of the UFL1–ENO1 interaction by the FDA‐approved antibiotic torezolid promotes ENO1 UFMylation, suppresses tumor metabolism, and sensitizes colorectal cancer to chemotherapy, revealing an actionable metabolic ...
Xiuqing Ma +14 more
wiley +1 more source