Results 91 to 100 of about 104,409 (252)

High-intensity UV laser ChIP-seq for the study of protein-DNA interactions in living cells

Nature Communications, 2017
Chromatin immunoprecipitation followed by DNA sequencing (ChIP-seq) can map transcription factor binding to a given genome. Here, Steube and colleagues devise a ChIP-seq technique based on photochemical crosslinking by high-intensity ultraviolet laser ...
Arndt Steube, Tino Schenk, Alexander Tretyakov, Hans Peter Saluz   +3 more
doaj   +1 more source

An efficient chromatin immunoprecipitation (ChIP) protocol for studying histone modifications in peach reproductive tissues. [PDF]

Plant Methods, 2022
Canton M, Farinati S, Forestan C, Joseph J, Bonghi C, Varotto S.   +5 more
europepmc   +1 more source

Function of the upstream activating factor in chromatin structure organization and transcriptional regulation at the yeast ribosomal DNA

, 2010
Chromatin is the template of all processes involved in DNA metabolism in the eukaryotic cell. Accordingly, chromatin is a dynamic structure which changes in its composition and posttranslational modification correlating with the functional state of a ...
Götze, Hannah
core   +1 more source

Dose‐Dependent Reprogramming of Chromatin Accessibility by SOX4 Drives the Transcriptional Response to Iron Overload

Advanced Science, EarlyView.
This study demonstrates that iron overload triggers widespread chromatin compaction and transcriptional repression in human granulosa cells, recapitulating features of endometriosis. The epigenetic reprogramming is orchestrated by a TFEB‐SOX4‐SWI/SNF axis, with SOX4 acting as a central, dosage‐sensitive regulator.
Feifei Li, Yaoqiu Wu, Guangyu Yang, Jingyi Lai, Xiaoyue Sun, Liyan Wang, Xiaoli Li, Jing Zhang, Qingxue Zhang, Hui Chen, Haiyan Lin, Bingxiang Xu, Junfeng Zhang, Hailong Wang, Anming Meng, Chunwei Cao   +15 more
wiley   +1 more source

Analysis of Protein–DNA Interaction by Chromatin Immunoprecipitation and DNA Tiling Microarray (ChIP-on-chip)

, 2017
Chromatin immunoprecipitation (ChIP) has become the most effective and widely used tool to study the interactions between specific proteins or modified forms of proteins and a genomic DNA region.
Zhao, C, Chunyan Zhao, Gao, H,, Hui Gao
core   +1 more source

Cigarette Smoke‐Induced Alveolar Macrophage Senescence via GEM/SIRT3‐Mediated Mitochondrial Dysfunction

Advanced Science, EarlyView.
Cigarette smoke accelerates the aging of immune cells in the lung. By combining human single‐cell sequencing, cell culture, and mouse models, the authors show that the protein GEM drives mitochondrial damage and senescence in alveolar macrophages by suppressing SIRT3.
Jin Wang, Meidan Wei, Xiangrong Song, Jiaxin Zhang, Lisha Zhang, Sixian Chen, Yaoyu Hu, Hui Gao, Jianxiang Li   +8 more
wiley   +1 more source

Microfluidic systems and methods for chromatin immunoprecipitation (ChIP)

, 2014
An integrated microfluidic chromatin immunoprecipitation assay dramatically improves the collection efficiency of ChIP DNA from cells. Immunoprecipitation of chromatin fragments is conducted in a microfluidic chamber with a large fraction of its volume ...

core  

ANKS1B in the Nucleus Accumbens Controls Escalated Cocaine Self‐Administration via Regulating CBP‐FoxO3 Complex

Advanced Science, EarlyView.
ANKS1B in the nucleus accumbens plays a critical role in the transition from controlled to escalated cocaine intake. Mechanistically, ANKS1B interacts with CBP to epigenetically suppress FoxO3 through H3K27 acetylation. The ANKS1B‐CBP‐FoxO3 signaling cascade presents a novel theraputic target for the treatment of cocaine addiction.
Liping Yang, Xiaoxuan Wu, Xuan Chen, Chao Peng, Zihang Li, Shumin Gao, Shiqiu Meng, Jing Dong, Dong Wu, Liying Lv, Ying Han, Yanxue Xue, Lin Lu, Jie Shi, Jianfeng Liu, Yan Sun   +15 more
wiley   +1 more source

CSA: a web service for the complete process of ChIP-Seq analysis

BMC Bioinformatics, 2019
Background Chromatin immunoprecipitation sequencing (ChIP-seq) is a technology that combines chromatin immunoprecipitation (ChIP) with next generation of sequencing technology (NGS) to analyze protein interactions with DNA.
Min Li, Li Tang, Fang-Xiang Wu, Yi Pan, Jianxin Wang   +4 more
doaj   +1 more source

CAST-ChIP Maps Cell-Type-Specific Chromatin States in the Drosophila Central Nervous System

Cell Reports, 2013
Chromatin organization and gene activity are responsive to developmental and environmental cues. Although many genes are transcribed throughout development and across cell types, much of gene regulation is highly cell-type specific.
Tamás Schauer, Petra C. Schwalie, Ava Handley, Carla E. Margulies, Paul Flicek, Andreas G. Ladurner   +5 more
doaj   +1 more source