Additional sex combs interacts with enhancer of zeste and trithorax and modulates levels of trimethylation on histone H3K4 and H3K27 during transcription of hsp70. [PDF]
, 2017 BACKGROUND: Maintenance of cell fate determination requires the Polycomb group for repression; the trithorax group for gene activation; and the enhancer of trithorax and Polycomb (ETP) group for both repression and activation.
Brock, Hugh W +4 more
core +2 more sources
Magnetic Beads Make Chromatin Immunoprecipitation Faster and Easier
BioTechniques, 2007 Active Motif's ChIP-IT™ Express Kits use protein G-coated magnetic beads instead of the traditionally used agarose beads to make chromatin immunoprecipitation (ChIP) faster and better than ever before.
John Heyman +3 more
doaj +1 more source
The laminA/NF-Y protein complex reveals an unknown transcriptional mechanism on cell proliferation [PDF]
, 2016 Lamin A is a component of the nuclear matrix that also controls proliferation by largely unknown mechanisms. NF-Y is a ubiquitous protein involved in cell proliferation composed of three subunits (-YA -YB -YC) all required for the DNA ...
Alonzi, Tonino +16 more
core +2 more sources
Direct identification of insulator components by insertional chromatin immunoprecipitation. [PDF]
PLoS ONE, 2011 Comprehensive understanding of mechanisms of epigenetic regulation requires identification of molecules bound to genomic regions of interest in vivo. However, non-biased methods to identify molecules bound to specific genomic loci in vivo are limited ...
Toshitsugu Fujita, Hodaka Fujii
doaj +1 more source
Improved nuclear localization of DNA-binding polyamides [PDF]
, 2006 Regulation of endogenous genes by DNA-binding polyamides requires effective nuclear localization. Previous work employing confocal microscopy to study uptake of fluorophore-labeled polyamides has demonstrated the difficulty of predicting a priori the ...
Baird +30 more
core +3 more sources
Native Chromatin Immunoprecipitation [PDF]
, 2011 Native chromatin immunoprecipitation refers to a method that allows for identification and quantification of DNA that is associated with specific chromatin proteins without altering the structure of these proteins. The method has been used with great success in the past and has some advantages over the more widely used cross-linking chromatin ...
Cosseau, Céline, Grunau, Christoph
openaire +3 more sources
Estradiol Receptor (ER) Chromatin Immunoprecipitation in MCF-7 Cells
Bio-Protocol, 2013 Steroid hormone receptors, for example estradiol receptor, act like transcription factors. In the cell, steroids bind to a specific receptor. Upon ligand binding, many steroid receptors dimerize and enter nuclei where they bind specific DNA sequences ...
Pia Giovannelli +2 more
doaj +1 more source
TBL1 is required for the mesenchymal phenotype of transformed breast cancer cells [PDF]
, 2019 The epithelial-to-mesenchymal transition (EMT) and its reversion (MET) are related to tumor cell dissemination and migration, tumor circulating cell generation, cancer stem cells, chemoresistance, and metastasis formation.
García Martínez, Jorge +4 more
core +2 more sources
Assessing the Epigenetic Status of Human Telomeres [PDF]
, 2019 The epigenetic modifications of human telomeres play a relevant role in telomere functions and cell proliferation. Therefore, their study is becoming an issue of major interest.
Vaquero Sedas, María Isabel +1 more
core +1 more source
Impact of chromatin structures on DNA processing for genomic analyses. [PDF]
PLoS ONE, 2009 Chromatin has an impact on recombination, repair, replication, and evolution of DNA. Here we report that chromatin structure also affects laboratory DNA manipulation in ways that distort the results of chromatin immunoprecipitation (ChIP) experiments. We
Leonid Teytelman +6 more
doaj +1 more source