Systematic comparison of monoclonal versus polyclonal antibodies for mapping histone modifications by ChIP-seq. [PDF]
, 2016 BackgroundThe robustness of ChIP-seq datasets is highly dependent upon the antibodies used. Currently, polyclonal antibodies are the standard despite several limitations: They are non-renewable, vary in performance between lots and need to be validated ...
Busby, Michele +11 more
core +1 more source
Allele-specific chromatin immunoprecipitation studies show genetic influence on chromatin state in human genome. [PDF]
PLoS Genetics, 2007 Several recent studies have shown a genetic influence on gene expression variation, including variation between the two chromosomes within an individual and variation between individuals at the population level.
Mitsutaka Kadota +7 more
doaj +1 more source
Tyrosine dephosphorylation of H2AX modulates apoptosis and survival decisions. [PDF]
, 2009 Life and death fate decisions allow cells to avoid massive apoptotic death in response to genotoxic stress. Although the regulatory mechanisms and signalling pathways controlling DNA repair and apoptosis are well characterized, the precise molecular ...
Cook, Peter J +5 more
core +2 more sources
RNA Chromatin Immunoprecipitation (RNA-ChIP) in Caenorhabditis elegans
Bio-Protocol, 2014 The RNA chromatin immunoprecipitation assay (RNA-ChIP) allows detection and quantification of RNA–protein interactions using in vivo cross-linking with formaldehyde followed by immunoprecipitation of the RNA–protein complexes.
Germano Cecere, Alla Grishok
doaj +1 more source
ChIPprimersDB: a public repository of verified qPCR primers for chromatin immunoprecipitation (ChIP) [PDF]
Nucleic Acids Research, 2019 Stefan Kurtenbach +2 more
exaly +2 more sources
TGFβ impairs HNF1α functional activity in Epithelial-to-Mesenchymal Transition interfering with the recruitment of CBP/p300 acetyltransferases [PDF]
, 2019 The cytokine transforming growth factor β (TGFβ) plays a crucial role in the induction of both epithelial-to-mesenchymal transition (EMT) program and fibro-cirrhotic process in the liver, where it contributes also to organ inflammation following several ...
Amicone, Laura +8 more
core +1 more source
ChIP on Chip: surprising results are often artifacts
BMC Genomics, 2010 Background The method of chromatin immunoprecipitation combined with microarrays (ChIP-Chip) is a powerful tool for genome-wide analysis of protein binding. However, a high background signal is a common phenomenon.
Skarstad Kirsten, Waldminghaus Torsten
doaj +1 more source
Chromatin immunoprecipitation to analyze DNA binding sites of HMGA2. [PDF]
PLoS ONE, 2011 BACKGROUND: HMGA2 is an architectonic transcription factor abundantly expressed during embryonic and fetal development and it is associated with the progression of malignant tumors.
Nina Winter +4 more
doaj +1 more source
The Snail repressor recruits EZH2 to specific genomic sites through the enrollment of the lncRNA HOTAIR in epithelial-to-mesenchymal transition [PDF]
, 2016 The transcription factor Snail is a master regulator of cellular identity and epithelial-to-mesenchymal transition (EMT) directly repressing a broad repertoire of epithelial genes.
A Kuzmichev +65 more
core +2 more sources
Tagging of MADS domain proteins for chromatin immunoprecipitation
BMC Plant Biology, 2007 Background Most transcription factors fulfill their role in complexes and regulate their target genes upon binding to DNA motifs located in upstream regions or introns.
van Zuijlen Lisette GC +4 more
doaj +1 more source