Results 21 to 30 of about 137,564 (241)
ChIP on Chip: surprising results are often artifacts
BMC Genomics, 2010 Background The method of chromatin immunoprecipitation combined with microarrays (ChIP-Chip) is a powerful tool for genome-wide analysis of protein binding. However, a high background signal is a common phenomenon.
Skarstad Kirsten, Waldminghaus Torsten
doaj +1 more source
Chromatin immunoprecipitation to analyze DNA binding sites of HMGA2. [PDF]
PLoS ONE, 2011 BACKGROUND: HMGA2 is an architectonic transcription factor abundantly expressed during embryonic and fetal development and it is associated with the progression of malignant tumors.
Nina Winter +4 more
doaj +1 more source
Magnetic Beads Make Chromatin Immunoprecipitation Faster and Easier
BioTechniques, 2007 Active Motif's ChIP-IT™ Express Kits use protein G-coated magnetic beads instead of the traditionally used agarose beads to make chromatin immunoprecipitation (ChIP) faster and better than ever before.
John Heyman +3 more
doaj +1 more source
Direct identification of insulator components by insertional chromatin immunoprecipitation. [PDF]
PLoS ONE, 2011 Comprehensive understanding of mechanisms of epigenetic regulation requires identification of molecules bound to genomic regions of interest in vivo. However, non-biased methods to identify molecules bound to specific genomic loci in vivo are limited ...
Toshitsugu Fujita, Hodaka Fujii
doaj +1 more source
Tagging of MADS domain proteins for chromatin immunoprecipitation
BMC Plant Biology, 2007 Background Most transcription factors fulfill their role in complexes and regulate their target genes upon binding to DNA motifs located in upstream regions or introns.
van Zuijlen Lisette GC +4 more
doaj +1 more source
Native Chromatin Immunoprecipitation [PDF]
, 2011 Native chromatin immunoprecipitation refers to a method that allows for identification and quantification of DNA that is associated with specific chromatin proteins without altering the structure of these proteins. The method has been used with great success in the past and has some advantages over the more widely used cross-linking chromatin ...
Cosseau, Céline, Grunau, Christoph
openaire +3 more sources
Estradiol Receptor (ER) Chromatin Immunoprecipitation in MCF-7 Cells
Bio-Protocol, 2013 Steroid hormone receptors, for example estradiol receptor, act like transcription factors. In the cell, steroids bind to a specific receptor. Upon ligand binding, many steroid receptors dimerize and enter nuclei where they bind specific DNA sequences ...
Pia Giovannelli +2 more
doaj +1 more source
Chromatin immunoprecipitation: optimization, quantitative analysis and data normalization
Plant Methods, 2007 Background Chromatin remodeling, histone modifications and other chromatin-related processes play a crucial role in gene regulation. A very useful technique to study these processes is chromatin immunoprecipitation (ChIP).
Peterhansel Christoph +5 more
doaj +1 more source
Impact of chromatin structures on DNA processing for genomic analyses. [PDF]
PLoS ONE, 2009 Chromatin has an impact on recombination, repair, replication, and evolution of DNA. Here we report that chromatin structure also affects laboratory DNA manipulation in ways that distort the results of chromatin immunoprecipitation (ChIP) experiments. We
Leonid Teytelman +6 more
doaj +1 more source
Titration-based normalization of antibody amount improves consistency of ChIP-seq experiments
BMC Genomics, 2023 Chromatin immunoprecipitation (ChIP) is an antibody-based approach that is frequently utilized in chromatin biology and epigenetics. The challenge in experimental variability by unpredictable nature of usable input amounts from samples and undefined ...
Ariel Caride +11 more
doaj +1 more source