Results 21 to 30 of about 153,188 (279)

Chromatin Immunoprecipitation [PDF]

Cold Spring Harbor Protocols, 2020
Chromatin immunoprecipitation, commonly referred to as ChIP, is a powerful technique for the evaluation of in vivo interactions of proteins with specific regions of genomic DNA. Formaldehyde is used in this technique to cross-link proteins to DNA in vivo, followed by the extraction of chromatin from cross-linked cells and tissues.
James, DeCaprio, Thomas O, Kohl
openaire   +3 more sources

Chromatin Immunoprecipitation from Caenorhabditis elegans Somatic Cells. [PDF]

Methods Mol Biol, 2020
Chromatin Immunoprecipitation is a regularly used method to detect DNA–protein interaction in diverse biological samples. Here we describe the application of ChIP for histone modifications in adult-stage Caenorhabditis elegans somatic ...
Pu M, Lee SS.
europepmc   +2 more sources

Chromatin Immunoprecipitation and Chromatin Immunoprecipitation with Massively Parallel Sequencing on Mouse Embryonic Tissue

, 2014
Regulation of gene expression must be tightly controlled during embryonic development. A central mechanism to control gene expression is the binding of sequence-specific transcription factors to cis-regulatory elements in the genome. Chromatin immunoprecipitation (ChIP) is a widely used technique to analyze binding of transcription factors and histone ...
Amin, Shilu, Bobola, Nicoletta
openaire   +4 more sources

Chromatin composition is changed by poly(ADP-ribosyl)ation during chromatin immunoprecipitation. [PDF]

PLoS ONE, 2012
Chromatin-immunoprecipitation (ChIP) employs generally a mild formaldehyde cross-linking step, which is followed by isolation of specific protein-DNA complexes and subsequent PCR testing, to analyze DNA-protein interactions.
Sascha Beneke, Kirstin Meyer, Anja Holtz, Katharina Hüttner, Alexander Bürkle   +4 more
doaj   +1 more source

Protocol for fractionation-assisted native ChIP (fanChIP) to capture protein-protein/DNA interactions on chromatin

STAR Protocols, 2021
Summary: Conventional chromatin immunoprecipitation (ChIP) includes many steps that need to be optimized. Here, we have described a protocol of fractionation-assisted native ChIP (fanChIP) which combines subfractionation and native ChIP to purify protein/
Ryo Miyamoto, Akihiko Yokoyama
doaj   +1 more source

Chromatin Immunoprecipitation in the Cnidarian Model System Exaiptasia diaphana

, 2023
Histone post-translational modifications (PTMs) and other epigenetic modifications regulate the chromatin accessibility of genes to the transcriptional machinery, thus affecting an organism's capacity to respond to environmental stimuli.
Della Valle, Francesco, Cui, Guoxin, Orlando, Valerio, Dix, Mascha Fiona, Aranda, Manuel, Liu, Peng   +5 more
core   +1 more source

p65 Chromatin Immunoprecipitation Protocol

Bio-Protocol, 2013
Chromatin Immunoprecipitation (ChIP) is an important procedure that allows you to verify if a certain protein is physically located at a regulatory region.
Crissy Dudgeon
doaj   +1 more source

Proteomic interrogation of human chromatin. [PDF]

, 2011
Chromatin proteins provide a scaffold for DNA packaging and a basis for epigenetic regulation and genomic maintenance. Despite understanding its functional roles, mapping the chromatin proteome (i.e. the "Chromatome") is still a continuing process. Here,
LeRoy, Gary, Christodoulos A Floudas, Floudas, Christodoulos A., Barry M Zee, Garcia, Benjamin A., Richard C Baliban, Baliban, Richard C., Sandra B Hake, Torrente, Mariana P., Zee, Barry M., Hake, Sandra B., Christodoulos A. Floudas, Barry M. Zee, Nicolas L. Young, Mariana P. Torrente, Gary LeRoy, Richard C. Baliban, Sandra B. Hake, Benjamin A. Garcia, Nicolas L Young, Benjamin A Garcia, Young, Nicolas L., Mariana P Torrente   +22 more
core   +1 more source

Accounting for immunoprecipitation efficiencies in the statistical analysis of ChIP-seq data [PDF]

, 2013
Background: ImmunoPrecipitation (IP) efficiencies may vary largely between different antibodies and between repeated experiments with the same antibody.
Bao, Yanchun,, ’t Hoen, Peter AC, Bao, Yanchun, Veronica Vinciotti, Hoen, P.A.C. 't, Vinciotti, V., Vinciotti, Veronica,, Yanchun Bao, Wit, Ernst,, Bao, Y.C., Ernst Wit, Wit, Ernst, Vinciotti, V, Peter AC ’t Hoen, 't Hoen, Peter A C, Vinciotti, Veronica, Hoen, Peter A.C. ’t,, Bao, Y, 't Hoen, PAC, Wit, E, Wit, E.   +20 more
core   +1 more source

Allele-specific chromatin immunoprecipitation studies show genetic influence on chromatin state in human genome. [PDF]

PLoS Genetics, 2007
Several recent studies have shown a genetic influence on gene expression variation, including variation between the two chromosomes within an individual and variation between individuals at the population level.
Mitsutaka Kadota, Howard H Yang, Nan Hu, Chaoyu Wang, Ying Hu, Philip R Taylor, Kenneth H Buetow, Maxwell P Lee   +7 more
doaj   +1 more source