Results 111 to 120 of about 519,147 (169)

DNA unwinding component of the nonhistone chromatin proteins [PDF]

open access: yes, 1976
A subclass of nonhistone chromatin proteins from rat liver, previously shown to exhibit high affinity for DNA, has been fractionated by single-stranded DNA-agarose affinity chromatography.
Patel, Gordhan L., Thomas, Terry L.
core  

Polyclonal Antibody Production against Mouse Purified IgG2a towards Use in Basic Research

open access: yesResearch in Molecular Medicine, 2016
Background: The ability of polyclonal antibodies to react with many epitopes of an antigen makes them valuable reagents in research and diagnosis.
Zahra Moradi nebrin   +8 more
doaj  

RNA Affinity Chromatography [PDF]

open access: yes, 2012
Martin Holcik, Nehal Thakor
core   +1 more source

PURIFICATION AND ANALYSIS OF CARBOHYDRATE CLUSTER FROM ERYTHROPOIETIN RECOMBINANT EXPRESSION RESULT ON THE LEAVEN SYSTEM OF Pichia pastoris

open access: yesMakara Seri Sains, 2011
For clinical purposes, pure protein and identification of carbohydrate structure from erythropoietin recombinant are needed. Purification was done with His-Trap affinity chromatography method and continued with gel filtration chromatography column to get
Adi Santoso, Andri Wardiana
doaj  

Clathrin structure characterized with monoclonal antibodies. I. Analysis of multiple antigenic sites. [PDF]

open access: yes, 1985
Three monoclonal antibodies that react with previously undefined antigenic determinants on the clathrin molecule have been produced and characterized. They were isolated from a fusion between myeloma cells and popliteal lymphocytes from SJL mice that had
Brodsky, Frances
core  

Protein purification by affinity chromatography [PDF]

open access: yes, 2012
Coelho, L. C. B. B.   +4 more
core   +1 more source

Transport property predictions for laser resonance chromatography on Rf$^+$ (Z = 104) [PDF]

open access: yesarXiv
We propose a theoretically designed laser resonance chromatography (LRC) experiment on Rf$^+$ (Z = 104) drifting in He buffer gas. To this end, we first developed a four-level rate equation model that simulates the optical pumping of Rf$^+$ from its ground state, $^2$D$_{3/2}$ (7s$^2$6d$^1$), to the metastable $^4$F$_{3/2}$ (7s$^1$6d$^2$) state via ...
arxiv  

Applicability of tandem affinity purification MudPIT to pathway proteomics in yeast [PDF]

open access: yes, 2004
A combined multidimensional chromatography-mass spectrometry approach known as "MudPIT" enables rapid identification of proteins that interact with a tagged bait while bypassing some of the problems associated with analysis of polypeptides excised from ...
Deshaies, Raymond J.   +6 more
core   +1 more source

A streamlined tandem affinity purification of His-MBP-SpyCas9, without buffer exchange, suitable for in vitro cleavage applications

open access: yesMethodsX
We present a streamlined protocol for the purification of recombinant doubly-tagged His-MBP-SpyCas9 protein utilizing a dual affinity chromatography approach whereby the elution volume from immobilized metal ion affinity chromatography (IMAC) is loaded ...
Filippo Fronza   +2 more
doaj  

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