Results 111 to 120 of about 519,147 (169)
DNA unwinding component of the nonhistone chromatin proteins [PDF]
A subclass of nonhistone chromatin proteins from rat liver, previously shown to exhibit high affinity for DNA, has been fractionated by single-stranded DNA-agarose affinity chromatography.
Patel, Gordhan L., Thomas, Terry L.
core
Polyclonal Antibody Production against Mouse Purified IgG2a towards Use in Basic Research
Background: The ability of polyclonal antibodies to react with many epitopes of an antigen makes them valuable reagents in research and diagnosis.
Zahra Moradi nebrin+8 more
doaj
For clinical purposes, pure protein and identification of carbohydrate structure from erythropoietin recombinant are needed. Purification was done with His-Trap affinity chromatography method and continued with gel filtration chromatography column to get
Adi Santoso, Andri Wardiana
doaj
Clathrin structure characterized with monoclonal antibodies. I. Analysis of multiple antigenic sites. [PDF]
Three monoclonal antibodies that react with previously undefined antigenic determinants on the clathrin molecule have been produced and characterized. They were isolated from a fusion between myeloma cells and popliteal lymphocytes from SJL mice that had
Brodsky, Frances
core
Protein purification by affinity chromatography [PDF]
Coelho, L. C. B. B.+4 more
core +1 more source
Transport property predictions for laser resonance chromatography on Rf$^+$ (Z = 104) [PDF]
We propose a theoretically designed laser resonance chromatography (LRC) experiment on Rf$^+$ (Z = 104) drifting in He buffer gas. To this end, we first developed a four-level rate equation model that simulates the optical pumping of Rf$^+$ from its ground state, $^2$D$_{3/2}$ (7s$^2$6d$^1$), to the metastable $^4$F$_{3/2}$ (7s$^1$6d$^2$) state via ...
arxiv
Applicability of tandem affinity purification MudPIT to pathway proteomics in yeast [PDF]
A combined multidimensional chromatography-mass spectrometry approach known as "MudPIT" enables rapid identification of proteins that interact with a tagged bait while bypassing some of the problems associated with analysis of polypeptides excised from ...
Deshaies, Raymond J.+6 more
core +1 more source
We present a streamlined protocol for the purification of recombinant doubly-tagged His-MBP-SpyCas9 protein utilizing a dual affinity chromatography approach whereby the elution volume from immobilized metal ion affinity chromatography (IMAC) is loaded ...
Filippo Fronza+2 more
doaj