Results 91 to 100 of about 529,464 (310)

In Vitro Maturation of a Humanized Shark VNAR Domain to Improve Its Biophysical Properties to Facilitate Clinical Development [PDF]

, 2017
Acknowledgments: The authors would like to acknowledge the funding support for this work from Scottish Enterprise [VNAR_001(2012)] and the Biotechnology and Biological Sciences Research Council (BB/K010905/1).Peer reviewedPublisher ...
Andrew J. Porter, Andrew J. Porter, Caroline Jane Barelle, Dawn Cornock, Gerard Donohoe, John Steven, Kenneth Saunders, Marina Kovaleva, Miguel F. Carvalho, Mischa R. Müller, Obinna C. Ubah, Thomas Baddeley   +11 more
core   +2 more sources

Highly Sensitive Electrochemical Biosensor Based on Hairy Particles with Controllable High Enzyme Loading and Activity

Advanced Functional Materials, EarlyView.
For the first time, a highly sensitive electrochemical biosensor based on SiO2‐based hairy particles with a grafted PDMAEMA polymer brush containing a quantifiable and large amount of immobilized Laccase is reported. The fabricated biosensor exhibits a sensitivity of 0.14 A·m⁻¹, a limit of detection (LOD) of 0.1 µm, and a detection range of 0.3–750 µm,
Pavel Milkin, Anila Antony, Hongtao Cai, Antonia Debevc, Ceyda Topal, Anne Linhardt, Alla Synytska, Leonid Ionov   +7 more
wiley   +1 more source

Feasibility of preparing human plasminogen by full chromatography from precipitation of Fraction Ⅲ in low temperature ethanol method

Zhongguo shuxue zazhi
[Objective] To determine the feasibility of preparing plasminogen (Pg) with Fraction Ⅲ precipitation (hereinafter referred to as FⅢ-P) from low-temperature ethanol process by full chromatography (hereinafter referred to as FⅢ-P process). [Methods] The FⅢ-
ZHANG Jin, YUE Shenglan, ZHU Chen, PENG Yan, ZHOU Yanxiang, LIN Lianzhen, CHEN Kejin, FENG Lu, HU Yong, ZHOU Zhijun   +9 more
doaj   +1 more source

Expression, Purification, and Biophysical Characterization of a Secreted Anthrax Decoy Fusion Protein in Nicotiana benthamiana. [PDF]

, 2017
Anthrax toxin receptor-mediated drug development for blocking anthrax toxin action has received much attention in recent decades. In this study, we produced a secreted anthrax decoy fusion protein comprised of a portion of the human capillary ...
Dandekar, Abhaya M, Duhra-Gill, Sifti, Kailemia, Muchena J, Karuppanan, Kalimuthu, Lebrilla, Carlito B, McDonald, Karen A, Nandi, Somen, Phu, My L, Rodriguez, Raymond L   +8 more
core   +2 more sources

SI‐bioATRP in Mesoporous Silica for Size‐Exclusion Driven Local Polymer Placement

Advanced Functional Materials, EarlyView.
An enzyme‐catalyzed surface‐initiated atom transfer radical polymerization (SI‐bioATRP) of an anionic monomer within mesoporous silica particles, using hemoglobin as a catalyst, allows for controlling the location of the formed polymer via size‐exclusion effects between the nanopores and the biomacromolecules, thereby opening routes to functional ...
Oleksandr Wondra, Eleonora Ornati, Robert Lehn, Joanna J. Mikolei, Hergen Breitzke, Raheleh Pardehkhorram, Gerd Buntkowsky, Nico Bruns, Annette Andrieu‐Brunsen   +8 more
wiley   +1 more source

A New Affinity Gel Synthesized for Phenylalanine Ammonia Lyase Isolated from Red Clover (Trifolium pratense L.) Leaf and an Investigation into Its Kinetic Properties

Separations
Phenylalanine ammonia lyase (PAL) was first purified using affinity chromatography from the leaves of red-flowered clover, a highly antioxidant source.
Yavuz Selim Toksöz, Çiğdem Bilen, Emine Karakuş   +2 more
doaj   +1 more source

Characterization of Tyrosinase Inhibitors in Dryopteris crassirhizoma Rhizome Using a Combination of High-Speed Counter-Current Chromatography, Affinity-Based Ultrafiltration, and Liquid Chromatography-Tandem Mass Spectrometry. [PDF]

Front Nutr, 2022
Wang Z, Wang N, Han D, Yan H.
europepmc   +1 more source

Applicability of tandem affinity purification MudPIT to pathway proteomics in yeast [PDF]

, 2004
A combined multidimensional chromatography-mass spectrometry approach known as "MudPIT" enables rapid identification of proteins that interact with a tagged bait while bypassing some of the problems associated with analysis of polypeptides excised from ...
Deshaies, Raymond J., Dunipace, Leslie A., Graumann, Johannes, McDonald, W. Hayes, Seol, Jae Hong, Wold, Barbara J., Yates, John R., III   +6 more
core   +1 more source

Tuning the Selectivity of the CO2 Reduction Reaction by Means of Synergistic Interactions in the Cu, Ag, Ce System

Advanced Functional Materials, EarlyView.
Multi‐metal Cu─Ce─Ag nanoparticles harness synergistic interactions to drive efficient electrochemical CO2 reduction reaction toward C2+ products at high current densities. Ag enhances CO production, Ce modulates Cu oxidation states, and together they boost *CO coverage and local pH to enhance C─C coupling, enabling record C2+ yields with suppressed ...
Nini Zhang, Muhammad Adib Abdillah Mahbub, Bashir Eid, Thomas Quast, Jian Zhang, Ridha Zerdoumi, Xin Wang, Sabine Seisel, Wangcheng Zhan, Wolfgang Schuhmann   +9 more
wiley   +1 more source

Evaluation of Glycated Albumin (GA) and GA/Hba1c Ratio for Diagnosis of Diabetes and Glycemic Control: A Comprehensive Review [PDF]

, 2017
Diabetes Mellitus (DM) is a group of metabolic diseases characterized by chronic high blood glucose concentrations (hyperglycemia). When it is left untreated or improperly managed, it can lead to acute complications including diabetic ketoacidosis and ...
Abdolrahim, Mojgan, Jazayeri, Hossein E., Rabiee, Mohammad, Rajab, Asodollah, Tahriri, Mohammadreza, Tayebi, Lobat, Yazdanpanah, Sara   +6 more
core   +3 more sources