Results 111 to 120 of about 1,193,888 (281)

Developing a process of lentivirus purification from recombinant fluids using peptide affinity ligands

Bioengineering & Translational Medicine
Lentiviral vectors (LVVs) are emerging as an enabling tool in gene and cell therapies, yet the toolkit for purifying them at scale is still immature. A pivoting moment in LVV isolation technology was marked by the introduction of affinity ligands for ...
Eduardo Barbieri, Gina N. Mollica, Sobhana A. Sripada, Shrirarjun Shastry, Yuxuan Wu, Arianna Minzoni, Will Smith, Elena Wuestenhagen, Annika Aldinger, Heiner Graalfs, Michael S. Crapanzano, Oliver Rammo, Michael M. Schulte, Michael A. Daniele, Stefano Menegatti   +14 more
doaj   +1 more source

Polymeric Sorbents as Energy‐Efficient Alternative to Cryogenic Distillation for Light Hydrocarbon Purification

Advanced Materials, EarlyView.
The transition from cryogenic distillation to polymeric sorbents for light hydrocarbon purification is crucial for energy and environmental sustainability. The polymeric sorbents are engineered to separate gas mixtures based on their specific properties.
Kelechi Festus, Ankit Mondal, Suman Mandal, Jazmine Aiya D. Marquez, Arnab Ghosh, Aishi Sikdar, Urme Podder, Ahana Sarkar, Qingsheng Wang, Hong‐Cai Zhou   +9 more
wiley   +1 more source

Split-Intein Mediated Affinity Chromatography for the Purification of a C-intein Tagged Protein

, 2018
The purification of biomolecules is a very challenging process due to the presence of a very complex mixture of other cell culture components. In order to achieve high protein yield and purity, a so-called affinity chromatography step is widely used [1].
Alberto Cavazzini, S. Felletti, Nicole Ulmer, M. Morbidelli, N. Ulmer, Oliver Rammo, Massimo Morbidelli, O. Rammo, A. Cavazzini, M. Schulte, Simona Felletti, Michael Schulte   +11 more
core  

Electrolyte Design for Fast‐Charging Lithium‐Based Batteries

Advanced Materials, EarlyView.
A decade of progress in fast‐charging electrolytes for lithium batteries is reviewed. Electrolyte design strategies spanning solvents, salts, additives, and advanced systems, such as localized high‐concentration electrolytes (LHCEs), are summarized. Advanced diagnostic tools for lithium plating and interphase chemistry are discussed, with perspectives ...
Chen Liu, Zehao Cui, Arumugam Manthiram
wiley   +1 more source

Adsorption‐Engineered Hydrocarbon Ionomers for Durable Proton‐Exchange Membrane Fuel Cells

Advanced Materials, EarlyView.
Hydrocarbon ionomers suffer from oxidation‐driven interfacial degradation in PEM fuel cell cathodes, leading to catalyst instability. Here, adsorption‐engineered poly(fluorene) ionomers decouple interfacial anchoring from oxidative degradation, enabling strong catalyst–ionomer interactions while resisting electrochemical oxidation.
Heemin Park, Kate Chen, Su Min Ahn, Hengquan Guo, Cy Fujimoto, Jong‐Ho Choi, Lynda Amichi, Danah Kim, Seung Geol Lee, Xiaojing Wang, Jacob S. Spendelow, Sun Young Kang, Panagiotis Bexis, Eun Joo S. Park, Yu Seung Kim   +14 more
wiley   +1 more source

Polyclonal Antibody Production against Mouse Purified IgG2a towards Use in Basic Research

Research in Molecular Medicine, 2016
Background: The ability of polyclonal antibodies to react with many epitopes of an antigen makes them valuable reagents in research and diagnosis.
Zahra Moradi nebrin, Jafar Majidi, Leili Aghebati Maleki, Tohid Kazemi, Jalal Abdolalizadeh, Somaieh Dadashi, Sadeg Eyvazi, Majid Ahmadi, Naiemeh Majidi zolbanin   +8 more
doaj  

Polyphenol‐Inspired Materials for Agricultural Applications

Advanced Materials, EarlyView.
This review outlines the use of polyphenol‐inspired materials for sustainable agriculture, highlighting their molecular design, interfacial assembly, structure–property relationships, and prospects toward precision agriculture, climate resilience, ecosystem protection, and circular bioeconomy strategies.
Haofu Liu, Omid Mazaheri, Zhixing Lin, Tianzheng Wang, Jingqu Chen, Yuang Gu, Xia Liang, Deli Chen, Frank Caruso   +8 more
wiley   +1 more source

Single step purification of plasmid DNA using peptide ligand affinity chromatography

, 2008
Single step affinity chromatography was employed for the purification of plasmid DNA (pDNA), thus eliminating several steps compared with current commercial purification methods for pDNA.
Han, Ying, Forde, Gareth M.
core   +1 more source

PURIFICATION AND ANALYSIS OF CARBOHYDRATE CLUSTER FROM ERYTHROPOIETIN RECOMBINANT EXPRESSION RESULT ON THE LEAVEN SYSTEM OF Pichia pastoris

Makara Seri Sains, 2011
For clinical purposes, pure protein and identification of carbohydrate structure from erythropoietin recombinant are needed. Purification was done with His-Trap affinity chromatography method and continued with gel filtration chromatography column to get
Adi Santoso, Andri Wardiana
doaj  

Controlled Synthesis of Tri‐ and Multi‐Doped Graphene

Advanced Materials Interfaces, EarlyView.
This review systematically evaluates synthesis routes for tri‐ and multi‐doped graphene, from hydrothermal and pyrolysis methods to flash Joule heating, critically assessing how each governs dopant incorporation, bonding configuration, and resulting electronic properties.
Maria Hasan, Alicja Bachmatiuk, Gražyna Simha Martynková, Karla Čech Barabaszová, Mark Hermann Rümmeli   +4 more
wiley   +1 more source