Results 81 to 90 of about 529,464 (310)

Selective purification of antibody-based protein scaffolds with a novel variable heavy chain 3 affinity resin

Journal of Chromatography Open
The evolution of biotherapeutics to effectively treat diseases has resulted in complex molecule scaffolds compared to traditional monoclonal antibodies (mAbs).
Anthony S. Ransdell, Melora Reed, John Herrington, Maria Hougland, Ronan M. Kelly   +4 more
doaj   +1 more source

Analyzing peptides and proteins by mass spectrometry: principles and applications in proteomics [PDF]

, 2012
Podeu consultar el llibre complet a: http://hdl.handle.net/2445/32166The study of proteins has been a key element in biomedicine and biotechnology because of their important role in cell functions or enzymatic activity. Cells are the basic unit of living
Estanyol i Ullate, Josep Maria, Fernández Vidal, Irene, Fidalgo, Maria José, Olmedo Casas, Eva   +3 more
core   +1 more source

Characterization of Cell Glycocalyx with Mass Spectrometry Methods. [PDF]

, 2019
The cell membrane plays an important role in protecting the cell from its extracellular environment. As such, extensive work has been devoted to studying its structure and function.
Lebrilla, Carlito B, Li, Qiongyu, Wong, Maurice, Xie, Yixuan   +3 more
core   +2 more sources

Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function

FEBS Open Bio, EarlyView.
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke, Michal Banasik, Rokas Juodeikis, Martin J. Warren, Richard W. Pickersgill   +4 more
wiley   +1 more source

Generation and in vivo characterization of a chimeric αvβ5-targeting antibody 14C5 and its derivatives [PDF]

, 2013
Background: Previous studies showed that radiolabeled murine monoclonal antibody (mAb) 14C5 and its Fab and F(ab')2 fragments, targeting αvβ5 integrin, have promising properties for diagnostic and therapeutic applications in cancer.
De Vos, Filip, Dumolyn, Caroline, Haustraete, Jurgen, Moerman, Lieselotte, Neyt, Sara, Schoonooghe, Steve   +5 more
core   +2 more sources

Mass spectrometry based identification of AMP‐O‐Tris generated by Thermococcus onnurineus Cas10

FEBS Open Bio, EarlyView.
Isolated Thermococcus onnurineus Cas10 generates the noncanonical ATP‐derived product AMP‐O‐Tris while in Tris‐containing buffer as identified via mass spectrometry, revealing relaxed nucleophile selectivity under isolated conditions. These findings suggest that multiprotein Csm complex assembly restricts Cas10 reactivity toward canonical cyclic ...
Su‐Jin Lee, Ga Seul Lee, JinHan Kim, Kwang‐Hyun Park, Seong‐Ryung Go, Jeong Hee Moon, Eui‐Jeon Woo   +6 more
wiley   +1 more source

Isolation and purification of HLA-DR antigen from Daudi cell line by immunoaffinity chromatography

Journal of Basic Research in Medical Sciences, 2017
Introduction: The major histocompatibility complex (MHC) is a group of cell surface proteins that are essential for recognizing foreign molecules in human and other mammals.
Zahra Khayyati, Fatemeh Yari
doaj  

Structural analysis of MDM2 RING separates degradation from regulation of p53 transcription activity [PDF]

, 2017
MDM2–MDMX complexes bind the p53 tumor-suppressor protein, inhibiting p53's transcriptional activity and targeting p53 for proteasomal degradation. Inhibitors that disrupt binding between p53 and MDM2 efficiently activate a p53 response, but their use in
A Plechanovová, A Plechanovová, Andreas K Hock, CS Chen, D Migliorini, D Shi, Danny T Huang, DC Scott, DE Christensen, Dominika Kowalczyk, E Branigan, E Meulmeester, Gary J Sibbet, H Dou, H Dou, H Kawai, H Kawai, I Ray-Coquard, J Momand, J Parant, JC Badciong, JD Oliner, JG Sanchez, JN Pruneda, K Itahana, K Linke, Karen H Vousden, KH Khoo, Koji Nomura, L Buetow, L Buetow, L Huang, LA Tollini, LC Storoni, LK Linares, LT Vassilev, M Andreeff, M Argentini, M Bista, M Kostic, Marta Klejnot, MG Koliopoulos, MHG Kubbutat, ML Choong, MS Dai, MS Dai, MV Poyurovsky, MW Jackson, N Minsky, N Zheng, P Dolezelova, P Emsley, P Roxburgh, PD Adams, PS Brzovic, Q Cheng, Q Cheng, Q Ding, R Honda, R Montes de Oca Luna, R Stad, R Stad, RA Finch, RK Geyer, S Iyappan, S Shangary, S Uldrijan, SN Jones, UM Moll, V Pant, W Kabsch, Y Pan   +71 more
core   +1 more source

Cyclic azapeptide CD36 ligand attenuates cardiac injury and reduces long‐chain fatty acid accumulation after myocardial ischemia–reperfusion in mice

FEBS Open Bio, EarlyView.
In a murine model of myocardial ischemia and reperfusion (MI/R), the CD36 azapeptide ligand MPE‐298 reduces cardiac injury and transiently lowers left ventricular long‐chain fatty acids (LCFAs) accumulation 3 h after reperfusion, accompanied by a decrease of oxidative stress and inflammation‐associated genes' expression in the heart and adipose tissue.
Jade Gauvin, Naghme Radmannia, David N. Huynh, Liliane Ménard, Caroline Daneault, Maïté Veilleux, Ahsanullah Ahsanullah, André C. Carpentier, William D. Lubell, Matthieu Ruiz, Huy Ong, Sylvie Marleau, Simon‐Pierre Gravel   +12 more
wiley   +1 more source

Reaction of Native and Denatured Brucella abortus (S19) Proteins with Antibody Using Affinity Chromatography and Immunoblotting

پزشکی بالینی ابن سینا, 2005
Western blotting or immunoblotting commonly use for study of reaction between antigens and antibodies. Denaturation of many proteins in immunoblotting can affect greatly the reactivity of antibodies and outcome of the procedure.
Ramin Karimi, Ali Mostafaie, Bahman Tabaraie, Yadollah Bahrami, Jalal Abdolalizadeh   +4 more
doaj