Results 61 to 70 of about 1,193,888 (281)
Modeling of protein adsorption in membrane affinity chromatography
, 2004 For the design of affinity membranes, protein adsorption in membrane affinity chromatography (MAC) was studied by frontal analysis. According to fast mass transfer, small thickness of affinity membranes and high affinity between the protein and the ...
Hao, WQ, Wang, JD, Chen, ZA, Liu, XL
core +1 more source
Glycan-specific whole cell affinity chromatography: A versatile microbial adhesion platform
MethodsX, 2014 We have sought a universal platform for elucidating and exploiting specificity of glycan-mediated adhesion by potentially uncharacterized microorganisms. Several techniques exist to explore microbial interactions with carbohydrate structures.
Maxwell L. Van Tassell +2 more
doaj +1 more source
FEBS Letters, EarlyView.Encapsulins are protein nanocompartments that play an important role in iron storage. In the Myxococcus xanthus encapsulin system, two cargo proteins called EncB and EncC contribute to iron mineralization. Here, we show that EncB and EncC generate iron‐containing minerals with distinct chemical compositions, suggesting that the composition of stored ...
Harry B. McDowell +2 more
wiley +1 more source
, 2007 A stationary phase for high performance affinity chromatography with immobilization of DNA onto silica gel was prepared and characterized. The effect of the ionic strength, concentration of Mg2+, EDTA and CH3 CN in the mobile phase on the retention of ...
Hu, Lianghai +5 more
core +1 more source
Production and Purification of a Polyclonal Antibody Against Purified Mouse IgG2b in Rabbits Towards Designing Mouse Monoclonal Isotyping Kits [PDF]
Advanced Pharmaceutical Bulletin, 2015 Purpose: Mouse IgG subclasses containing IgG1, IgG2a, IgG2b and IgG3 have been defined and described both physiochemically and immunologically. Methods: Sepharose beads conjugated with protein A affinity chromatography was used for purification of mouse
Sadeq Eivazi +8 more
doaj +1 more source
Molecular Oncology, EarlyView.The novel styrylquinazolinone‐based molecule W1B effectively suppresses glioblastoma by inhibiting IGF1R and EGFR. In high‐glucose microenvironments driving tumor resistance, W1B acts synergistically with the EGFR inhibitor dacomitinib. This combination safely blocks compensatory survival signaling in zebrafish xenograft models. Showcasing promising in
Patryk Rurka +9 more
wiley +1 more source
Molecular Oncology, EarlyView.Breast cancer remains a major cause of cancer death in women, frequently developing endocrine therapy resistance. This study demonstrates that upregulated p21‐activated kinase 1 (PAK1) activity drives resistance to tamoxifen and long‐term estrogen deprivation in ER+ breast cancer models.
Luisa Schwarzmüller +10 more
wiley +1 more source
Integrated scale down of the primary downstream purification process and assessment of alternative process options for the production of an ovine polyclonal antibody based anti-venom. [PDF]
, 2005 This thesis describes the development of a linked 1000th scale mimic of four distinct unit operations, including precipitation and centrifugation. These operations are utilised during the production of an anti-snake venom Fab fragment. A new approach has
Neal, G.E.
core
Versatile vector tools for efficient protein screening across multiple expression systems
FEBS Open Bio, EarlyView.A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu +5 more
wiley +1 more source
Characterisation of chromatography adsorbents for antibody bioprocessing [PDF]
, 2009 Currently the purification of monoclonal antibodies for therapeutic purposes is reliant on protein A affinity chromatography. The rapid growth of this class of therapeutic and their high value makes the understanding of protein A chromatography an ...
Sheth, B.
core