Results 71 to 80 of about 333,536 (310)

Integrated scale down of the primary downstream purification process and assessment of alternative process options for the production of an ovine polyclonal antibody based anti-venom.

, 2005
This thesis describes the development of a linked 1000th scale mimic of four distinct unit operations, including precipitation and centrifugation. These operations are utilised during the production of an anti-snake venom Fab fragment. A new approach has
Neal, G.E.
core  

Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function

FEBS Open Bio, EarlyView.
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke, Michal Banasik, Rokas Juodeikis, Martin J. Warren, Richard W. Pickersgill   +4 more
wiley   +1 more source

Reaction of Native and Denatured Brucella abortus (S19) Proteins with Antibody Using Affinity Chromatography and Immunoblotting

پزشکی بالینی ابن سینا, 2005
Western blotting or immunoblotting commonly use for study of reaction between antigens and antibodies. Denaturation of many proteins in immunoblotting can affect greatly the reactivity of antibodies and outcome of the procedure.
Ramin Karimi, Ali Mostafaie, Bahman Tabaraie, Yadollah Bahrami, Jalal Abdolalizadeh   +4 more
doaj  

Characterisation of chromatography adsorbents for antibody bioprocessing

, 2009
Currently the purification of monoclonal antibodies for therapeutic purposes is reliant on protein A affinity chromatography. The rapid growth of this class of therapeutic and their high value makes the understanding of protein A chromatography an ...
Sheth, B.
core  

Purification of tobacco ribonuclease by affinity chromatography [PDF]

, 1971
RESP ...
Jervis, L.
core   +1 more source

Mass spectrometry based identification of AMP‐O‐Tris generated by Thermococcus onnurineus Cas10

FEBS Open Bio, EarlyView.
Isolated Thermococcus onnurineus Cas10 generates the noncanonical ATP‐derived product AMP‐O‐Tris while in Tris‐containing buffer as identified via mass spectrometry, revealing relaxed nucleophile selectivity under isolated conditions. These findings suggest that multiprotein Csm complex assembly restricts Cas10 reactivity toward canonical cyclic ...
Su‐Jin Lee, Ga Seul Lee, JinHan Kim, Kwang‐Hyun Park, Seong‐Ryung Go, Jeong Hee Moon, Eui‐Jeon Woo   +6 more
wiley   +1 more source

Feasibility of preparing human plasminogen by full chromatography from precipitation of Fraction Ⅲ in low temperature ethanol method

Zhongguo shuxue zazhi
[Objective] To determine the feasibility of preparing plasminogen (Pg) with Fraction Ⅲ precipitation (hereinafter referred to as FⅢ-P) from low-temperature ethanol process by full chromatography (hereinafter referred to as FⅢ-P process). [Methods] The FⅢ-
ZHANG Jin, YUE Shenglan, ZHU Chen, PENG Yan, ZHOU Yanxiang, LIN Lianzhen, CHEN Kejin, FENG Lu, HU Yong, ZHOU Zhijun   +9 more
doaj   +1 more source

Direct analysis – no sample preparation – of bioavailable cortisol in human plasma by weak affinity chromatography (WAC)

, 2017
Pre-analytical treatment of blood plasma is a time consuming and often rate limiting step in the workflow of LC/MS analysis. We present in this pilot study a new approach for quantitative LC/MS based on weak affinity chromatography (WAC) of crude plasma.
Torres, Anthony R.,, Sten Ohlson, Kaur, Jagjit,, Boehm, Bernhard,, Niss, Ulf,, Drum, Chester Lee,, Bernhard Boehm, Manfred Raida, Tim Bengala, Anthony R. Torres, Jagjit Kaur, Raida, Manfred,, Ulf Niss, Chester Lee Drum, Ohlson, Sten,, Bengala, Tim,   +15 more
core   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

A New Affinity Gel Synthesized for Phenylalanine Ammonia Lyase Isolated from Red Clover (Trifolium pratense L.) Leaf and an Investigation into Its Kinetic Properties

Separations
Phenylalanine ammonia lyase (PAL) was first purified using affinity chromatography from the leaves of red-flowered clover, a highly antioxidant source.
Yavuz Selim Toksöz, Çiğdem Bilen, Emine Karakuş   +2 more
doaj   +1 more source